黏虫CYP9A113基因的克隆及外源物质对基因表达的诱导效应

黏虫是我国作物上最重要的害虫之一。细胞色素P450能够参与昆虫外源物质代谢。本研究采用RACE技术克隆了一条编码黏虫P450基因的cDNA序列,并通过Real-time PCR技术,检测了4种外源物质对该基因表达的诱导效应。该基因被国际P450命名委员会命名为CYP9A113,GenBank登录号为KY436739。利用2.5%高效氯氟氰菊酯乳油的LD_(50)处理黏虫3 h,LD_(10)、LD_(30)和LD_(50)处理12 h和24 h,可诱导表达CYP9A113基因;20%氯虫苯甲酰胺悬浮剂的LD_(10)处理黏虫12、24和48 h,LD_(30)和LD_(50)处理24 h,CYP9A113基因表达呈诱导效应;0.1和0.5 mg/mL香豆素处理6、12、24和48 h,CYP9A113基因表达均呈诱导效应;0.1和0.5 mg/mL吲哚-3-甲醇处理3、6、12、24和48 h,CYP9A113基因表达均呈诱导效应。     

Mitigating nitrous oxide emissions from agricultural soils by precision management

Nitrous oxide (N₂O) emissions make up a significant part of agricultural greenhouse gas emissions. There is an urgent need to identify new approaches to the mitigation of these emissions with emerging technology. In this short review four approaches to precision managements of agricultural systems are described based on examples of work being undertaken in the UK and New Zealand. They offer the opportunity for N₂O mitigation without any reduction in productivity. These approaches depend upon new sensor technology, modeling and spatial information with which to make management decisions and interventions that can both improve agricultural productivity and environmental protection.     

健康状况不佳是导致咬尾的一个危险因素

正在多篇系列文章中,顶级的动物福利研究人员解释了他们在国际项目"Fare Well Dock"内获得的结果。该计划调查如何才能不用进行断尾。挪威和芬兰的研究人员深入探讨了健康和咬尾之间的关系——这些现象是相互关联的吗?咬尾有害于动物福利,会对生产者的经济收益产生负面的影响。我们知道,咬尾是一个由多种因素引发的问题,营养、饲喂方案、猪圈设计、饲养密度和玩具的供应情况都会影响发生咬尾的净风险。健康状况不佳也被认为是一个重要的风险因素,但是现有的证据尚不足以证明两者之辛基大     

Why cuckoos should parasitize parrotbills by laying eggs randomly rather than laying eggs matching the egg appearance of parrotbill hosts?

The coevolutionary interaction between cuckoos and their hosts has been studied for a long time, but to date some puzzles still remain unsolved. Whether cuckoos parasitize their hosts by laying eggs randomly or matching the egg morphs of their hosts is one of the mysteries of the cuckoo problem. Scientists tend to believe that cuckoos lay eggs matching the appearance of host eggs due to selection caused by the ability of the hosts to recognize their own eggs.In this paper, we first review previous empirical studies to test this mystery and found no studies have provided direct evidence of cuckoos choosing to parasitize host nests where egg color and pattern match. We then present examples of unmatched cuckoo eggs in host nests and key life history traits of cuckoos, e.g. secretive behavior and rapid egg-laying and link them to cuckoo egg laying behavior. Finally we develop a conceptual model to demonstrate the egg laying behaviour of cuckoos and propose an empirical test that can provide direct evidence of the egg-laying properties of female cuckoos. We speculate that the degree of egg matching between cuckoo eggs and those of the host as detected by humans is caused by the ability of the hosts to recognize their own eggs, rather than the selection of matching host eggs by cuckoos. The case of Common Cuckoos(Cuculus canorus) and their parrotbill hosts(Paradoxornis alphonsianus), where it has been shown that both have evolved polymorphic eggs(mainly blue and white), was used to develop a conceptual model to demonstrate why cuckoos should utilize parrotbill hosts by laying eggs randomly rather than laying eggs matching the appearance of host eggs.In conclusion, we found no evidence for the hypothesis that cuckoos lay eggs based on own egg color matching that of the parrotbill-cuckoo system. We argue theoretically that laying eggs matching those of the hosts in this system violates a key trait of the life history of cuckoos and therefore should be maladaptive.     

Changes in various metabolic parameters in blood and milk during experimental Escherichia coli mastitis for primiparous Holstein dairy cows during early lactation

Background:The objective of this study was to characterize the changes in various metabolic parameters in blood and milk during IMI challenge with Escherichia coli(E.coli) for dairy cows during early lactation.Thirty,healthy primiparous Holstein cows were infused(h = 0) with-20-40 cfu of live ? coli into one front mammary quarter at ~4-6wk in lactation.Daily feed intake and milk yield were recorded.At-12,0,3,6,12,18,24,36,48,60,72,96,108,120,132,144,156,168,180 and 192 h relative to challenge rectal temperatures were recorded and quarter foremilk was collected for analysis of shedding of E.coli.Composite milk samples were collected at-180,-132,-84,-36,-12,12,24,36,48,60,72,84,96,132 and 180 h relative to challenge(h = 0) and analyzed for lactate dehydrogenase(LDH),somatic cell count,fat,protein,lactose,citrate,beta-hydroxybutyrate(BHBA),free glucose(fglu),and glucose-6-phosphate(G6P).Blood was collected at-12,0,3,6,12,18,24,36,60,72,84,132 and 180 h relative to challenge and analyzed for plasma non-esterified fatty acids(NEFA),BHBA and glucose concentration.A generalized linear mixed model was used to determine the effect of IMI challenge on metabolic responses of cows during early lactation.Results:By 12 h,E.coli was recovered from challenged quarters and shedding continued through 72 h.Rectal temperature peaked by 12 h post-challenge and returned to pre-challenge values by 36 h post-IMI challenge.Daily feed intake and milk yield decreased(P 0.05) by 1 and 2 d,respectively,after mastitis challenge.Plasma BHBA decreased(12 h;P 0.05) from 0.96 ± 1.1 at 0 h to 0.57 ±0.64 mmol/L by 18 h whereas concentration of plasma NEFA(18 h) and glucose(24 h) were significantly greater,11 and 27%,respectively,after challenge.In milk,fglu,lactose,citrate,fat and protein yield were lower whereas yield of BHBA and G6 P were higher after challenge when compared to pre-challenge values.Conclusions:Changes in metabolites in blood and milk were most likely associated with drops in feed intake and milk yield.However,the early rise in plasma NEFA may also signify enhanced adipose tissue lipolysis.Lower concentrations of plasma BHBA may be attributed to an increase transfer into milk after IMI.Decreases in both milk lactose yield and%after challenge may be partly attributed to reduced conversion of fglu to lactose.Rises in G6 P yield and concentration in milk after challenge(24 h) may signify increased conversion of fglu to G6 P.Results identify changes in various metabolic parameters in blood and milk after IMI challenge with E.coli in dairy cows that may partly explain the partitioning of nutrients and changes in milk components after IMI for cows during early lactation.     

Feeding preferences of mesograzers on aquacultured Gracilaria and sympatric algae

While large grazers can often be excluded effectively from algal aquaculture operations, smaller herbivores such as small crustaceans and gastropods may be more difficult to control. The susceptibility of three Gracilaria species to herbivores was evaluated in multiple-choice experiments with the amphipod Ampithoe ramondi and the crab Acanthonyx lunulatus. Both mesograzers are common along the Mediterranean coast of Israel. When given a choice, the amphipod preferred to consume Gracilaria lemaneiformis significantly more than either G. conferta or G. cornea. The crab, however, consumed equivalent amounts of G. lemaneiformis and G. conferta, but did not consume G. cornea. Organic content of these algae, an important feeding cue for some mesograzers, could not account for these differences. We further assessed the susceptibility of a candidate species for aquaculture, G. lemaneiformis, against local algae, including common epiphytes. When given a choice of four algae, amphipods preferred the green alga Ulva lactuca over Jania rubens. However, consumption of U. lactuca was equivalent to those of G. lemaneiformis and Padina pavonica. In contrast, the crab showed a marked and significant preference for G. lemaneiformis above any of the other three algae offered. Our results suggest that G. cornea is more resistant to herbivory from common mesograzers and that, contrary to expectations, mixed cultures or epiphyte growth on G. lemaneiformis cannot reduce damage to this commercially appealing alga if small herbivores are capable of recruiting into culture ponds. Mixed cultures may be beneficial when culturing other Gracilaria species.     

Soil carbon characterization along the profile of two forest soils under Quercus pyrenaica

In light of concerns over climate change and increasing levels of CO2 in the atmosphere,it is of importance to investigate soil organic matter in Mediterranean forests at a profile scale.In-depth studies of the organic fraction are also of interest to improve understanding of carbon balance and to facilitate modelling of carbon fixation in forest soils.This research evaluates the relationships between diverse parameters such as colour,content,and form of soil organic matter(SOM).Two Quercus pyrenica ecosystems with soils classified as inceptisols with a xeric or dry moisture regime,and developed under a Mediterranean climate in Spain,were used to characterize SOM through the complete sequence of layers of the soil profile.The differentiating factor between the two ecosystems was slope gradient.Characterization was done using characteristics of humic substances(HS)as indicators of SOM turnover in inceptisols.Infrared analysis was used to further characterize the humic acids.As soil colour measurements are a tool for soil type classification and soil organic carbon prediction,the relation between HS colour measured by reflection and by transmission was determined in order to establish a relationship between measurement techniques.Infrared analysis and colour provided evidence of a different level of stabilization of HS from both soils,and between the different horizons.Oxidation of humic acids was found to be greater in deeper horizons than in the surface layers.An inverse relationship between HS colour measured by reflection and by transmission was revealed.Both soils showed a clear trend in which horizons presenting lower absorbance numbers showed higher figures of hue and value.A more marked accumulation of humified compounds was found in pedons,(the smallest unit or volume of soil that contains all the soil types),in the less steep slope.This might be explained in terms of the physiographic position affecting infiltration behavior and exposure to runoff.     

蜘蛛酶对水奶牛产奶量、乳成分、体细胞数的影响

选取处于泌乳期中后期的水奶牛20头,按照胎次、泌乳天数与产奶量相近或相似的原则分为对照组和试验组,对照组奶牛饲喂基础日粮,试验组在基础日粮基础上每头水奶牛每日额外添加2g蜘蛛酶,研究其对水奶牛生产性能的影响。结果表明,整个试验期水奶牛产奶量都呈下降趋势,但是试验组降低幅度小于对照组;对照组体细胞数在试验结束时下降了25.90%,而试验组下降了47.53%,因此在牛乳体细胞数较低的水平下,在基础日粮中添加蜘蛛酶仍能起到比较明显的效果;另外,蜘蛛酶还可以提高乳蛋白和乳脂肪,并显著降低乳中活菌总数。     

绿肥能提高茶树的越冬性

乌克藏苏维埃社会主义共和国科学院植物园曾经进行了三年的试验,研究营养对茶树风土驯化的影响。试验设在外喀尔巴阡省穆卡契夫区拉夫卡村的地段上。那里一年中有9个月气温在零度以上,这保证有长的生长期。根据多年的资料,年平均温度是9.5°,最冷月(一月)平均温度1.7°同时这个月