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Samy M ELEAWA Mahmoud A ALKHATEEB Fahaid H ALHASHEM Ismaeel BIN-JALIAH Hussein F SAKR Hesham M ELREFAEY Abbas O ELKARIB Riyad M ALESSA Mohammad A HAIDARA Abdullah S. SHATOOR Mohammad A KHALIL 《The Journal of reproduction and development》2014,60(2):115-127
This study was performed to investigate the protective and therapeutic effects of
resveratrol (RES) against CdCl2-induced toxicity in rat testes. Seven
experimental groups of adult male rats were formulated as follows: A) controls+NS, B)
control+vehicle (saline solution of hydroxypropyl cyclodextrin), C) RES treated, D)
CdCl2+NS, E) CdCl2+vehicle, F) RES followed by CdCl2
and M) CdCl2 followed by RES. At the end of the protocol, serum levels of FSH,
LH and testosterone were measured in all groups, and testicular levels of TBARS and
superoxide dismutase (SOD) activity were measured. Epididymal semen analysis was
performed, and testicular expression of Bcl-2, p53 and Bax was assessed by RT-PCR. Also,
histopathological changes of the testes were examined microscopically. Administration of
RES before or after cadmium chloride in rats improved semen parameters including count,
motility, daily sperm production and morphology, increased serum concentrations of
gonadotropins and testosterone, decreased testicular lipid peroxidation and increased SOD
activity. RES not only attenuated cadmium chloride-induced testicular histopathology but
was also able to protect against the onset of cadmium chloride testicular toxicity.
Cadmium chloride downregulated the anti-apoptotic gene Bcl2 and upregulated the expression
of pro-apoptotic genes p53 and Bax. Resveratrol protected against and partially reversed
cadmium chloride testicular toxicity via upregulation of Bcl2 and downregulation of p53
and Bax gene expression. The antioxidant activity of RES protects against cadmium chloride
testicular toxicity and partially reverses its effect via upregulation of BCl2 and
downregulation of p53 and Bax expression. 相似文献
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The objectives of the present study were to determine the effects of season on some semen parameters and bacterial contamination of Awassi ram semen. Semen samples from six mature Awassi rams were used in this study. Semen collection was performed with artificial vagina every week, from September 2009 to October 2010. Volume, sperm concentration, mass motility, individual motility, percentage live sperm and sperm abnormalities were evaluated. Moreover, determination of viable bacterial count of the rams was also recorded weekly. Higher (p < 0.05) semen volume in the hot summer and spring months was observed of August (1.55 ± 0.08 ml) and March (1.27 ± 0.15 ml). Sperm concentration was highest (p < 0.05) in the breeding season (late summer to early autumn) of September (4.21 ± 0.86 × 10(9) sperm/ml). Sperm individual motility and percent of live sperm observed in August (summer) and May (end of spring) when the environmental temperature started to increase were recorded highest values and differed significantly (p < 0.05) from December and January (winter). The highest value of the mean sperm acrosomal defects (13.33 ± 0.63%) was recorded in December. The highest value of the mean viable bacterial count (138.3 ± 21.6) was recorded in July (summer). A significant decrease (p < 0.01) in the mean viable bacterial count was observed from the middle of winter towards the end of spring. The lowest bacterial count was noted in January (60.5 ± 2.98). It could be concluded from the results of the present study that there is an effect of season on ram semen quality, and summer high temperature in northern Iraq has no effect on Awassi ram semen. There is a significant effect of season on bacterial count on Awassi ram semen. 相似文献
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