Integrated effect of potassium humate and α-tocopherol applications on soil characteristics and performance of Phaseolus vulgaris plants grown on a saline soil

Soil salinity is one of the major problems of agriculture that limits plant performance, particularly in arid and semiarid regions. Therefore, the effect of potassium humate (KH) and α-tocopherol (TOC), used singly or in integration, on soil characteristics, and on plant performance, physio-biochemical attributes and antioxidative defense system of Phaseolus vulgaris L. plants grown under salt stress (EC = 6.35–6.42 dS m^?1) was investigated. Half g KH kg^?1 soil was used as soil amendment before sowing and 1.0 mM TOC was used as foliar spray twice; at 25 and 40 days after sowing. Results showed that, KH significantly improved soil physical and chemical properties, which positively reflected on plant growth and productivity, physio-biochemical attributes, mineral nutrients (N, P, K and Ca), osmoprotectants (soluble sugars and proline), non-enzymatic (ascorbic acid, glutathione and TOC) and enzymatic (superoxide dismutase, catalase and guaiacol peroxidase (GPOX)) antioxidants compared to untreated controls. The single TOC foliar application recorded the same positive results of KH. Integrated KH + TOC treatment was most effective compared to the single treatments. The above results recommended benefits of this integrated KH + TOC for the possibility of sustainable agronomic performance of common beans grown on saline soils.     

Natural bee-honey based biostimulants confer salt tolerance in onion via modulation of the antioxidant defence system

Plant biostimulants are an emerging trend of crop management products which can enhance nutrient uptake, plant growth and productivity under various abiotic stresses. The ability of diluted bee-honey extract (DHE) to enhance the tolerance of onion plants to salinity stress has been investigated. Two-season field experiments were consecutively conducted in 2015/16 and 2016/17 to study the effect of 25–50 g/L DHE foliar application on growth, yield, physio-biochemical attributes, and antioxidative defence of two onion cultivars (i.e. Giza^Red and Giza-20) grown on a saline soil (EC = 8.81 dS m^?1). Results exhibited that DHE significantly increased biomass production, bulb yield and water use efficiency, leaf photosynthetic and pigments contents. Additionally, DHE applicati\on also improves osmoprotectants, membrane stability index (MSI) and relative water content (RWC), and enzymatic and non-enzymatic antioxidants of both onion cultivars in comparison with untreated control plants. In general, both cultivars showed a similar response to the DHE. Results of this study highlighted the potential impact of DHE as a promising plant bio-stimulant for overcoming the harmful effects of soil salinity stress by increasing the activity of plant antioxidative defence systems     

Diet and dog characteristics affect major and trace elements in hair and blood of healthy dogs

Veterinary Research Communications - Obtaining correct amounts of essential elements, and avoiding toxic metals are key factors in dog health. Through analyzing major and trace elements in hair and...     

West Nile virus infection in horses in Saudi Arabia (in 2013–2015)

West Nile virus (WNV) is an important emerging zoonotic arbovirus giving rise to clinical syndromes of varying severity in humans and horses. Culex mosquitoes are the main vector. Although WNV has been reported in many countries in the Middle East and Asia, little is known about its prevalence in equine populations in the Arabian Peninsula. We have carried out a serological study on 200 horses to assess WNV infection in the Eastern and Central regions of Saudi Arabia in 2013–2015. Sera were tested for the presence of WNV antibodies in parallel using a commercial enzyme‐linked immunosorbent assay (ELISA) kit and microneutralization (MN) tests. In comparison with the MN assay used as “gold standard,” we find the ELISA had a sensitivity of 94.7% and specificity of 80.1%. The prevalence of WNV neutralizing antibody ranged from 5 (17.3%) of 29 sera collected in Riyadh up to 15 (55.6%) of 27 sera collected from Al‐Qateef. These findings highlight the need to be aware of the possibility of WNV disease in humans and horses presenting with central nervous system disease in the Kingdom of Saudi Arabia.     

Isolation and characterization of <Emphasis Type="Italic">Serratia rubidaea</Emphasis> from dark brown spots of tomato fruits

Bacterial contamination of fresh tomato fruits is of great concern. From naturally infected tomato fruits showing dark brown irregularly shaped spots, 36 bacterial isolates were recovered and identified on phenotypic characteristics and sequences of the gene encoding the 16S rRNA. Five isolates showing spots on tomato fruits in the pathogenicity test with healthy tomato fruits belong to the genus Serratia on the basis of phenotypic characteristics. One representative isolate of these has been further identified as a Serratia rubidaea by sequencing of the 16S rRNA gene. This is the first evidence showing that a S. rubidaea strain can cause spots on tomato fruits. Virulence of the S. rubidaea was also confirmed by the production and secretion of a large variety of enzymes capable of degrading the complex polysaccharides of the plant cell wall and membrane constituents. Nineteen bacterial isolates of the 36 did not induce any spot symptoms in a pathogenicity test on artificially infected tomato fruits although these are known as phytopathogenic bacteria. Five of these 19 bacterial isolates were identified as Ralstonia species on the basis of biochemical tests. Sequencing of the 16S ribosomal gene of one representative isolate revealed that the isolate is closely related to Ralstonia solanacearum. Six isolates of the 19 were related to Xanthomonas vesicatoria on the basis of biochemical tests and eight were related to the Enterobacteriaceae. One representative isolate of the Enterobacteriaceae could be identified by the 16S rRNA gene as Enterobacter cloacae subsp. dissolvens. The 12 other strains were related to Proteus mirabilis based on the 16S RNA gene sequence of one representative isolate. The isolates related to P. mirabilis did not produce any symptoms on artificially infected tomato fruits. The nucleotide sequences of S. rubidaea strain E9, E. cloacae strain E23, P. mirabilis strain E11, and R. solanacearum strain E15 have been deposited in the GenBank nucleotide sequence database under accession numbers HM585373 to HM585376.     

Influence of a pyrethroid insecticide on soil fungi

The selective effect of the pyrethroid insecticide (Polytrin) on soil fungi was studied at three doses when incorporated in soil or in agar or liquid medium. Polytrin induced an inhibitory effect on soil fungi after 2, 5 and 40 days when applied at certain levels and a stimulatory effect after 20 days of treatment with the high dose. When incorporated in the agar medium, Polytrin decreased the total counts of fungi, particularly, Aspergillus and Penicillium at the high dose (10.0 ppm). In liquid medium, it showed no significant effect except in case of A. terreus which was significantly enhanced by the three doses.