Detection of Candida species by nested PCR method in one-humped camels (Camelus dromedarius)

Tropical Animal Health and Production - Systemic fungal diseases are the infections caused by false treatment protocols and generally are not taken into consideration especially in the veterinary...     

Effect of temperature and photoperiod on development and fecundity of an acarophagous ladybird beetle,Stethorus gilvifrons

The development, fecundity and survival ofStethorus gilvifrons Mulsant (Coleoptera: Coccinellidae) fed onTetranychus cinnabarinus Boisduval (Acari: Tetranychidae) were recorded at three constant temperatures (20, 25 and 30±1°C) and 50±10% relative humidity, under two photoperiods (16:8 L:D and 8:16 L:D) produced using artificial light (4000 lux). The development rate for the egg stage (r_[Te]) increased linearly with increasing temperature (r_[Te]=0.0132*T ? 0.0955; R²=0.95). The theoretical egg-development threshold was estimated to be 7.24°C; 75.75 degree-days (DD) were required for hatching. The total development time (r_[Tt]) also decreased linearly with increasing temperature (r_[Tt]=0.0039*T ? 0.0325; R²=0.98). The development threshold was estimated to be 8.33°C and full development from egg to adult required 256.41 DD. Higher temperatures resulted in a shorter generation time (T ₀) and decreased net reproductive rate (R ₀). The length of the previposition and postoviposition period, as well as longevity, decreased significantly with increasing temperature under both photoperiods. The oviposition and postoviposition periods, longevity, and total fecundity were not significantly affected by photoperiod. The values of both the intrinsic rate of increase (r _m ) andR ₀ were highest under the long-day photoperiod at 25°C. The mortality rate was lowest at 20°C under the short-day photoperiod. Of the conditions tested, the optimum temperature for rearingS. gilvifrons was 25°C and the optimum photoperiod was 16:8 L:D.     

Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.)

The treated seeds (control, KNO₃ and hydropriming) of sunflower (Helianthus annuus L.) cultivar Sanbro were evaluated at germination and seedling growth for tolerance to salt (NaCl) and drought conditions induced by PEG-6000 at the same water potentials of 0.0, −0.3, −0.6, −0.9 and −1.2 MPa. Electrical conductivity (EC) values of the NaCl solutions were 0.0, 6.5, 12.7, 18.4 and 23.5 dS m⁻¹, respectively. The objective of the study was to determine factors responsible for germination and early seedling growth due to salt toxicity or osmotic effect and to optimize the best priming treatment for these stress conditions.

Results revealed that germination delayed in both solutions, having variable germination with different priming treatments. Germination, root and shoot length were higher but mean germination time and abnormal germination percentage were lower in NaCl than PEG at the same water potential. Seeds were able to germinate at all concentrations of NaCl but no seed germination was observed at −1.2 MPa of PEG treatments. NaCl had less inhibitor effect on seedling growth than the germination. It was concluded that inhibition of germination at the same water potential of NaCl and PEG resulted from osmotic effect rather than salt toxicity. Hydropriming increased germination and seedling growth under salt and drought stresses.     

Organic synthesis of new putative lycopene metabolites and preliminary investigation of their cell-signaling effects

Tomato is the main dietary source of lycopene, a carotenoid that is known to have protective effects on health and whose metabolites could also be involved in bioactivity. Herein we present the first organic synthesis of two potentially bioactive lycopene metabolites, namely, 10'-apolycopen-10'-oic acid (6) and 14'-apolycopen-14'-oic acid (13), which were obtained in their (all-E) stereoisomeric forms using Wittig and Horner-Wadsworth-Emmons type coupling reactions. Both molecules are shown to up-regulate the carotenoid asymmetric cleavage enzyme BCO2 while having no effect on BCO1 expression.     

Alternative Marketing Strategies for Honey and Other Bee Products in Turkey

ABSTRACT

Turkey is the fourth leading honey producing country in the world after China, the United States, and Argentina, with around 5.35% of the market. One of the most important problems facing the Turkish beekeeping sector is marketing. In general, the market structure for animal products in Turkey has a confusing chain, which causes great differences between producer and consumer prices. Beekeepers have several alternatives for marketing their honey in Turkey: they may sell directly to consumers (in local markets or roadside stands), to a honey cooperative, to a wholesaler, to a honey packer or dealer, and/or to the export market. This study examines the marketing channels for honey and other bee products in Turkey, discusses the problems and defects of the current system from the perspective of producers and other actors in the market, and suggests solutions that include an increased role for cooperatives and extension services.     

A stereological study on calculation of volume values regarding lumbosacral segments of quails

In the study, total segment, grey matter and white matter volume values of lumbosacral segment of spinal cord in quails (Coturnix coturnix japonica) were stereologically examined. Regardless of sex, six adult quails were used as material. After the materials were perfused, they were dissected and their spinal cords were uncovered. Tissue samples were taken from each lumbosacral segment of spinal cord. After the tissue fixation process, 6–7 sections having a 5‐µm thickness were separated in every 50th section as from the first section, where the tissue was seen, among tissue samples of each segment. These segments were stained with haematoxylin–eosin staining. They were photographed via a microscope. By using the Cavalieri's principle, the volume values of both the total segment, grey matter (GM) and white matter (WM) in each lumbosacral segment were separately calculated. All these calculations were performed by using SHTEREOM 1.5 program. As a result, total volume, grey matter and white matter volume values of lumbosacral segments and white matter/total volume, grey matter/total volume, and grey matter/white matter volume fractions were obtained.     

A stereological analysis on the calculation of the volume values of thoracic segments in ducks

Stereology is a method for examining two-dimensional objects as three-dimensional objects. The aim of this study was to offer volume values for thoracic segments in ducks by means of stereological methods. This study examined the data obtained from stereological analysis of the total volume and grey and white matter volume values of the thoracic segment, a part of the adult duck spinal cord with a weight of 3–4 kg. In the study, study samples consisted of 10 adult ducks (Anas) used without gender discrimination. To perfuse all of the animals, 10% formaldehyde was utilised. The perfused animals were kept in 10% formaldehyde for one week. The spinal cord was uncovered following the removal of arcus vertebrae parts of thoracic vertebrae in the thoracic part of dissected ducks. Tissue samples of thoracic segments were taken; 5-µm-thick cross sections from these tissue samples were taken via microtome. Attention was paid to obtaining samples at the ratio of 1/250 by taking 12 cross sections from each segment. The cross sections were subjected to haematoxylin-eosin staining. Photographs of all cross sections were taken using a microscope. The volume values of all tissue and grey and white matter structures in each thoracic segment of the spinal cord were calculated. The total volume, grey and white matter volume densities in thoracic segments of ducks, as well as the data obtained as a result of proportioning volume values of one another are represented in the conclusion section of this study.     

The effects of planting systems on soil biology and quality attributes of tomatoes

This study was carried out to determine the effects of seedling types (grafted and non-grafted) and different plantation systems (raised-bed and flat planting) on growth, yield and quality of tomato (Solanum lycopersicum cv. Depar F1) plants organically grown in open-field conditions in Samsun. Soil microbial biomass-C increased by 25% and soil CO₂ contents increased by 16% in raised-bed systems. It was observed that soil microbial biomass-C positively correlated with CO₂ contents, leaf chlorophyll content, stomatal conductance and yield (P < 0.05 and P < 0.01) and negatively correlated with soil compaction. The highest chlorophyll content (47.37 CCI), fruit shape index (1.21) and yield (1.95 kg plant^?1) were obtained from grafted seedlings of the raised-bed planting systems. The highest stomatal conductance (118.50 mmol m^?2 s^?1) and firmness (79.34%) were obtained from non-grafted seedlings of the raised-bed planting systems. As a result, successful tomato cultivation was carried out with the use of raised-bed and grafted seedlings. However, non-grafted seedlings of the raised-beds had higher yield and quality values than the grafted seedlings of the flat planting.     

The Turkish Mezzes Formulated with Protein-Rich Green Sea Vegetable (Chlorophyta), Ulva rigida,Cultured in Onshore Tank System

Eating macroalgae (seaweeds) is considered an unusual activity in Turkey, although macroalgae have been known and prized for their nutritional purposes for many years in the Orient. The present study examines the addition of macroalgae to Turkish daily diets. Most Turkish meals begin with mezze, which is literally “a pleasant taste” and is probably derived from the Arabic word mezaq, which means “the taste, the savor of a thing.” The green macroalga Ulva rigida Agardh 1824 (Chlorophyta) was used to develop alternative protein-rich Turkish mezzes, using otherwise traditional recipes.

U. rigida was reared in tanks. The yield of U. rigida in an onshore tank system demonstrated the potential of U. rigida to produce marine biomass. The U. rigidas were used in macroalga-based mezzes. The macroalga substituted grape leaves, spinach, lamb’s lettuce, salicornia, or lettuce and resulted in higher protein content than those vegetables.

The prepared Ulva mezzes were tested in regard to taste, smell, color, texture, and general appearance and scored “pretty good” and “perfect.” The results suggest that Ulva can be produced and utilized for protein rich mezzes in Turkey.