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1.
Effect of Relaxin on Fertility Parameters of Frozen–Thawed Buffalo (Bubalus bubalis) Sperm 下载免费PDF全文
AR Elkhawagah V Longobardi G Neglia A Salzano G Zullo GA Sosa G Campanile B Gasparrini 《Reproduction in domestic animals》2015,50(5):756-762
The aim of this work was to evaluate the effect of relaxin on fertility parameters of buffalo frozen/thawed sperm. Sperm were incubated in the absence of capacitating agents (negative control), with a known capacitating agent such as heparin (positive control) and with 50 and 100 ng/ml relaxin for 2 and 4 h. Sperm viability, motility, capacitation and the effect of relaxin on the fertilizing ability after heterologous IVF were evaluated. Although viability was not affected, relaxin increased (p < 0.05) sperm motility compared to the negative and positive controls both after 2 h (60.0 ± 2.0, 60.0 ± 3.1, 68.3 ± 1.7 and 69.4 ± 2.7, respectively, in negative control, positive control, 50 and 100 ng/ml relaxin) and 4 h (55.0 ± 2.5, 53.3 ± 3.0, 62.2 ± 3.0 and 65.0 ± 3.2, respectively, in negative control, positive control, 50 and 100 ng/ml relaxin) incubation. When sperm were incubated with both 100 ng/ml relaxin and heparin, a decrease (p < 0.01) of pattern A, that is low capacitation level, was observed compared to the negative control both after 2 h (54.4, 34.3 and 36.4%, respectively, in negative control, positive control and 100 ng/ml relaxin) and 4 h (51.9, 35.0 and 34.3%, respectively, in negative control, positive control and 100 ng/ml relaxin). Moreover, an increase (p < 0.01) of pattern EA, that is high capacitation level, was recorded with 100 ng/ml relaxin and heparin compared to the negative control both after 2 h (44.1, 59.3 and 57.7%, respectively, in negative control, positive control and 100 ng/ml relaxin) and after 4 h (43.0, 54.4 and 56.0%, respectively, in negative control, positive control and 100 ng/ml relaxin). Finally, relaxin increased (p < 0.01) cleavage rate compared to the negative control (57.1 ± 4.4, 72.5 ± 6.0, 71.4 ± 5.5 and 73.6 ± 2.9, respectively, in negative control, positive control, 50 and 100 ng/ml relaxin). In conclusion, relaxin has a beneficial effect on motility, capacitation and fertilizing ability of frozen–thawed buffalo sperm. 相似文献
2.
Robson RV Alves Tatiana Soares Elinaldo FL Bento Ricardo S Roldan‐Filho Brbara SS Souza Marcele KN Lima Jssica S Nascimento Luana CBB Coelho Roberto A S Thmarah A Lima Gabriel GA Gonalves Fbio A Brayner Luiz C Alves Daniela MAF Navarro Thiago H Napoleo Patrícia MG Paiva 《Pest management science》2020,76(2):730-736
3.
研究了不同条件下大豆水溶性多糖溶液的黏度变化情况,同时和其他常用的稳定剂如果胶等也做了比较。试验结果表明。本实验室生产的大豆水溶性多糖的水溶液的黏度随着温度的升高、浓度的降低、外加盐量的增加而降低;随着外加蔗糖量、pH值的增加而增加;相比较于其他稳定剂大豆水溶性多糖的黏度很低,使酸乳饮料具有清爽的口味。 相似文献
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The great European spruce bark beetle (Dendroctonus micans),which had for many years been seen as a potential threat toBritain's spruce plantations, was first discovered in Britainduring August 1982, breeding in Sitka spruce. Surveys in spruceplantations were initiated to determine the distribution ofthe beetle, a necessary background for development of controlstrategies. This paper reports the development and evolutionof survey techniques from 1982 to 1989, as well as the spreadof the D.micans populations during this time. Details of controlstrategies and legislation to contain the outbreak are alsogiven, along with conclusions concerning the natural dispersalof D.micans. Received 23 July 1990. 相似文献
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为研究甘南地区欧拉羊杂交改良山谷型藏羊效果,对欧拉羊与山谷型藏羊杂交试验组和山谷型藏羊本交对照组所产后代的初生、3月龄、6月龄、12月龄、18月龄的体尺、体重进行了跟踪测定,并对不同年龄段杂交后代与对照组后代进行了对比分析和差异显著性检验。结果表明:杂交F1代初生、3月龄、6月龄、12月龄、18月龄的体尺、体重极显著的高于对照组(P〈0.01),初生、18月龄管围差异显著,3月龄、6月龄、12月龄管围差异不显著(P〉0.05)。 相似文献
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Soil matric suctions under a crop of ryegrass on farmyard manure-treated and untreated plots were determined over a total period of 24 weeks from March to November. The soil moisture characteristic of each plot was determined five times throughout this period, and for each plot and on every occasion a linear relationship was found between moisture content and log matric suction. A formula was derived to account for the seasonal changes in moisture characteristic and it was then possible to obtain matric suction values from the soil moisture contents obtained from twice-weekly sampling of each plot. Although differences between available-water capacity of the manured and unmanured plots were small throughout the 6-month period of sampling, the soil matric suctions of the manured plots were almost always lower than those of the unmanured plots. The lower suctions prevailing in the manured soil could be a factor contributing to the higher yields of ryegrass obtained from the manured plots as compared with those obtained from the unmanured plots. 相似文献