排序方式: 共有1条查询结果,搜索用时 15 毫秒
1
1.
Michel KERE Chawalit SIRIBOON Neng-Wen LO Ngoc Tan NGUYEN Jyh-Cherng JU 《The Journal of reproduction and development》2013,59(1):78-84
In this study, a dose-response assessment was performed to understand the relation
between supplementation of media with L-ascorbic acid or vitamin C and porcine oocyte
maturation and the in vitro development of parthenotes (PA) and handmade
cloned (HMC) embryos. Various concentrations (0, 25, 50 and 100 µg/ml) of vitamin C
supplemented in in vitro maturation (IVM) and culture (IVC) media were
tested. None of these vitamin C additions affected nuclear maturation of oocytes, yet
supplementation at 50 µg/ml led to significantly increased intracellular glutathione (GSH)
levels and reduced reactive oxygen species (ROS). When cultured in IVM- and/or
IVC-supplemented media, the group supplemented with 50 µg/ml of vitamin C showed improved
cleavage rates, blastocyst rates and total cell numbers per blastocyst (P<0.05)
compared with other groups (control, 25 µg/ml and 100 µg/ml). In contrast, supplementation
with 50 µg/ml vitamin C decreased (P<0.05) the apoptosis index as compared with the
groups supplemented with 100 µg/ml. In addition, even with a lower blastocyst rate to
start with (37.6 vs. 50.3%, P<0.05), supplementation of HMC embryos
with vitamin C ameliorated their blastocyst quality to the extent of PA embryos as
indicated by their total cell numbers (61.2 vs. 59.1). Taken together, an
optimized concentration of vitamin C supplementation in the medium not only improves
blastocyst rates and total cell numbers but also reduces apoptotic indices, whereas
overdosages compromise various aspects of the development of parthenotes and cloned
porcine embryos. 相似文献
1