应用细菌磁颗粒实时荧光RT-PCR检测南瓜花叶病毒

 A novel real-time RT-PCR method, BMPs based real-time RT-PCR which integrated with magne-tic separation technique of bacterial magnetic particles(BMPs), was set up for detection of Squash mosaic virus(SqMV).After SqMV particles in crude sap were concentrated by BMPs, viral RNAs were released and detected by real time RT-PCR.The results indicated that BMPs based real-time RT-PCR was efficient, and the detection sensibility was equivalent to that of the Trizol based real-time RT-PCR, of which Trizol reagent was used for viral RNAs extration.Comparing to Trizol-based method, the BMPs-based method had advantages of simplicity on operation, time saving for RNA extraction and without using noxious organic chemicals.     

北京口岸进境伴侣动物隔离检疫的现状及思考

本文介绍了北京口岸进境伴侣动物隔离检疫的现状,包括动物隔离检疫监管体系、动物隔离场的职能和隔离检疫的流程。并通过具体实践,分析了隔离检疫期间存在的问题,并提出相应建议。