江苏省丰县苹果生产绿色防控现状调查与分析

为了解2017—2018年丰县地区苹果生产及绿色防控现状,在丰县梁寨、师寨、大沙河以及宋楼4个镇的13个苹果种植村,调研农户的基本信息、生产经营情况以及果园病虫害防治及管理情况。调查结果表明,丰县地区的苹果种植户主要负责人多为男性,且年龄整体偏大,总体文化水平较低,其中同时具备党员或村干部资质的人数较少;在气候正常年份（2017年）,丰县地区苹果平均产量可达到 29178.6 kg/hm²,平均售价2.4元/kg,平均收入73608元/hm²;而在遭遇极端气候影响的年份（2018年）,苹果的产量锐减50%以上,平均售价上涨约1.8倍,但农户总体收入减少;农药与肥料的投入是生产资料成本的最重要构成部分,均占到总投入的40%左右,其构成比重受生产资料成本波动的影响较小;现阶段丰县地区苹果园中最高发的病害为斑点落叶病、轮纹病,腐烂病以及炭疽病也有一定程度危害,高发的虫害为梨小食心虫、绣线菊蚜、山楂叶螨以及绿盲蝽;针对病虫害,所有苹果种植户均采用化学防治的方法,而使用农业防治、物理防治以及生物防治措施进行综合防治的农户比例不高;大部分种植户喷药次数集中在9~12次/年,首次喷药时间集中在3月末4月初,末次喷药时间各有不同;农资店为种植户农药的主要购买渠道,且大部分种植户选择由农资店代为配制,其中过半数的种植户选择根据当年各自果园中的情况喷施;丰县地区苹果喷药机械化程度较高,目前小型全自动化机动式喷雾器覆盖率已超过80%。     

山东栒子全长转录组测序数据组装及功能注释

山东栒子是中国山东省的特有种,现已处于极度濒危状态。目前,山东栒子的分子生物学研究较少,基因数据库资源极度缺乏,急需探究其生物学遗传信息,以加快对山东栒子的保护遗传学工作。本研究以山东栒子叶片、花、成熟果实为实验材料,利用PacBio Sequel测序平台对其转录组进行全长转录组测序。共得到高质量去冗余的转录本53932个,作为最终转录本序列。预测到的CDS区共52490个;对其SSR位点进行分析,对测序得到Unigenes进行单核苷酸至六核苷酸重复的SSR位点搜索,共搜索到26796个SSR位点。对非冗余转录本利用BLAST软件与NR、Swissprot、GO、COG、KOG、KEGG 6个数据库进行比对,一共成功注释了53319个Unigenes,其中与NR数据库进行比对中注释为苹果的的相关基因数量最多,其次是白梨和桃;在GO数据库中有33305条山东栒子Unigenes被注释分类,由生物学过程、细胞成分和分子功能三部分组成;在与COG数据库比对中,共有23910条比对到了同源序列,且一共被分为25类;在与KEGG数据库的一系列比对中,可将Unigenes映射到126条代谢通路中。本研究在高通量全长转录组水平对山东栒子进行了系统研究,这为进一步开展山东栒子的分子标记开发和挖掘优良基因提供了科学依据,从而推动山东栒子的保护与利用。     

黄海双船变水层拖网网囊的网目选择性研究

为研究黄海双船变水层拖网网囊的网目选择性,2015年10月在黄海南部进行了网囊网目为40、54、60mm和70mm的套网拖网试验,采用Logistic选择模型和体周估算法对网囊网目选择性进行研究。结果表明,黄海双船变水层拖网渔获主要为蓝点马鲛、银鲳、带鱼、小黄鱼和鳀鱼;随着网囊网目尺寸的增大,逃逸率明显增加;极大似然法估算的模型参数,用AIC值检验拟合良好;估算出以变水层拖网捕捞达到可捕标准的部分经济鱼类相应的网囊网目尺寸,分别为小黄鱼71.0 mm、银鲳103.6mm、带鱼92.3mm、蓝圆鲹63.8mm。在黄海目前的渔业资源现状下,为保护小黄鱼、带鱼和鲐鲹等中小型经济鱼类的渔业资源,建议将双船变水层拖网的最小网囊网目尺寸定为60mm。     

Apparent digestibility coefficients of animal and plant feed ingredients for juvenile Pseudobagrus ussuriensis

A study was conducted to determine apparent digestibility coefficients (ADCs) of dry matter (DM), crude protein (CP), lipid, gross energy, phosphorus and amino acids (AAs) from Peruvian fish meal (PFM), native fish meal (NFM), meat bone meal (MBM), dried daphnia meal (DDM), soybean meal (SBM), full‐fat soybean (FSBM), cottonseed meals (CSM), rapeseed meal (RSM) and corn gluten meal (CGM) for Pseudobagrus ussuriensis (7.8 ± 0.06 g). Test diets were formulated to contain a 70:30 mixture of reference diet to test ingredient with chromic oxide (5 g kg^–1) serving as the inert marker. ADCs of DM ranged from 49.94% (CGM) to 87.11% (PFM), the highest ADCs of DM were observed in PFM and the lowest was found in CGM. ADCs of CP in the test ingredients ranged from 74.92% (CGM) to 93.41% (SBM). ADCs of crude lipid ranged from 51.80% (CGM) to 94.07% (PFM) for fish. ADCs of energy ranged from 56.42% (CGM) to 90.09% (PFM). ADCs of phosphorus ranged from 21.81% (CSM) to 65.70% (PFM). AA availability for the test ingredients followed a similar pattern to ADCs of CP, in which values for PFM, NFM and MBM were generally higher (p < .05) than those of other protein ingredients. Among all plant meals, the AA availability values in SBM were higher (p < .05) than those in CGM, CSM and RSM. The availability of most AAs in CGM was the lowest (p < .05) among all tested ingredients. In conclusion, PFM and NFM are good sources of available protein and AAs. When fishmeal supply is limited, MBM and DDM are substitutes for fishmeal reduction in P. ussuriensis diets; at the same time, SBM and FSBM are also very acceptable protein feedstuffs. However, CSM, RSM and CGM could not be used at high levels in P. ussuriensis diets because of their inferior digestibilities.     

白马鸡人工驯养初步研究

通过人工饲养的方式探索出白马鸡驯养的经验,即:在野生白马鸡适宜生境下建立饲养场,从育雏阶段开始进行人工投食饲养和本地土鸡带领相结合的驯养方式。目前已经完成从子一代到子二代的完整驯养、饲养流程,取得了云南省林业厅的驯养繁殖许可证。对育雏、亚成体、成体阶段的喂食量、存活情况等作了分析,认为接近原始生境的环境及本地带雏母鸡是成功的重要条件。     

侧挂式电动移栽机系统开发

现有的大田使用的蔬菜苗移栽机，体积庞大，作业半径较大，动力一般为内燃机，作业时有废气排出，很难直接应用于温室内作业。侧挂式电动移栽机系统采用水平侧挂结构，尺寸小，转弯灵活；以直流电动机作为移栽动力，无废气排出，不会对温室内空气环境造成污染；更适合温室内移栽作业，不仅可以提高种植效率，也有利于降低劳动强度和劳动成本。      

5TYS280玉米脱粒清选试验台的设计研究

我国夏播玉米主要集中在黄淮海地区,其生长期短、收获时籽粒含水率高,直接脱粒收获易造成籽粒破碎,脱净率与籽粒破碎率和含杂率之间的矛盾,作业质量较难保证。目前,针对高含水率玉米脱粒清选装置的系统理论与试验研究均较少,因此设计开发了一种玉米脱粒清选试验台。其主要由机架、脱粒分离装置、清选装置、输送装置、电机控制及转速数据采集系统等部件组成。以籽粒破碎率和含杂率为评价指标,通过调整滚筒转速、滚筒倾角、凹板间隙、筛网倾角、曲轴转速及风机转速等关键因素水平,进行单因素多水平试验及多因素多水平正交试验,确定高含水率玉米脱粒清选装置的最佳参数组合,为玉米籽粒收获机脱粒清选部件设计、改进及参数选择提供依据。     

Response of labile organic C and N pools to plastic film removal from semiarid farmland soil

To examine the effects of plastic film removal on grain yield and soil organic matter (SOM), a spring maize (Zea may L.) field experiment was conducted for 5 yr at Changwu Agricultural and Ecological Experimental Station of Northwest China. Compared with traditional plastic film mulching during entire growing stages (FM), plastic film removal at the silking stage (RM) resulted in a 6.3% higher average maize yield. Under the RM treatment, soil organic carbon and total nitrogen significantly increased after the 5‐yr cultivation in the 0‐ to 20‐cm layer. Significant increases in extractable organic C (EOC), KMnO₄‐oxidizable C (KMnO₄‐C) and C management index (CMI) in the 0‐ to 20‐cm layer, and light fraction organic C and EOC in the 20‐ to 40‐cm layer were observed in response to plastic film removal after the 1‐yr treatment; the responses were more significant after 5 yr. Under the RM treatment, significant increases in microbial biomass C, light fraction organic N, extractable organic N, KMnO₄‐C and CMI were also observed after five years in the 20‐ to 40‐cm layer. Moreover, KMnO₄‐C and EOC were much more sensitive than other labile SOM fractions to the application of RM, even after only 1 yr of cultivation. Therefore, compared with mulching for the whole growing season, plastic film removal at the maize silking stage is an effective option for increasing yields and enhancing SOM concentration and soil sustainability in the regions with semiarid monsoon climates that have sufficient rainfall during maize reproductive stages.     

木本植物逆境胁迫研究进展

木本植物(Woody plant)具有多年生、木质部发达和茎坚硬等特点,能抵御复杂多变的外界环境。本综述归纳了木本植物逆境胁迫的几个重要方面,例如盐胁迫、干旱胁迫和低温胁迫。归纳并总结了与应激有关的基因、蛋白质、转录因子和应激反应机制,并简要概述了木本植物在植被保护、园林绿化和土壤改良等方面的应用价值,最后对木本植物研究的局限性和存在的不足进行了讨论。蒙古柳(Salix linearistipularis)作为松嫩平原盐碱地区唯一具有群落分布的木本植物,是研究逆境胁迫的重要树种材料。因此,本综述着重介绍了耐盐碱木本植物蒙古柳对盐等逆境胁迫的研究进展,以期为木本植物逆境胁迫研究提供一定的理论基础;并针对目前研究存在的不足,为进一步研究提供了参考方向。