紫花槭秋季叶色表达转录组初步分析

为了丰富紫花槭转录组数据,进一步开展紫花槭秋季叶片呈色机制研究.本研究以紫花槭秋季转色期三个阶段(前期,中期,后期)叶片为材料,采用高通量测序技术进行转录组初步分析.转录组数据共获得50501条Unigene,有35316条Unigene在数据库中得到注释,其中NR数据库中注释到的Unigene数量最多,共35024条,占69.4％.在注释到的物种中,紫花槭比对的Unigene与甜橙(Citrus sinensis)相似度最高,共有4290条,占12.25％.紫花槭转录组中的Unigene根据GO功能可分为生物学过程、细胞组分和分子功能3大类,共有25375条,其中生物学过程的基因最多,主要聚集于代谢过程和细胞过程等.基于Unigene库的基因结构分析,其中SSR分析共获得12711个SSR标记,占Unigene总数的36％.SSR位点共包含150种重复基元,单碱基重复所占比例最高(7184个,61.86％),四碱基重复、五碱基重复和六碱基重复所占比例较低.Unigene库中共有328239个SNP位点,发生频率为1/190 bp,SNP位点分为转换和颠换两种类型的碱基替换方式,其中碱基转换位点213787个(65.13％),碱基颠换位点114452个(34.87％),碱基转换类型发生频率高于颠换类型.6种单碱基变异中,2种转换类型A/G、C/T的发生频率分别为33.03％和32.10％;4种颠换类型中A/T发生频率最高,为11.52％;C/G发生频率最低,为5.79％.紫花槭转录组秋季叶色表达的转录组分析,可为紫花槭叶色基因调控、定向分子育种和培育彩叶新品种提供研究提供基础的数据信息.     

Quantitative trait loci for fruit size and flowering time-related traits under domestication and diversifying selection in cucumber (Cucumis sativus)

In cucumber, the genetic basis of traits under domestication and/or diversifying selection is not well understood. Here, we reported QTL mapping for flowering time and fruit size-related traits with segregating populations derived from a cultivated × wild cross. Phenotypic data of flowering time (FT), fruit size (FS), fruit number (FN) and fruit weight per plant (FW) were collected in multiple environments. QTL analysis identified 19 QTL for these traits. We found that the major-effect QTL FT1.1 played an important role in regulating flowering time in cultivated cucumber, whereas the minor-effect QTL FT6.3 contributed to photoperiod sensitive flowering time during domestication. Two novel consensus FS QTL, FS1.4 and FS2.3, seem to be the targets of selection during breeding for the US processing cucumber. All other FS QTL were co-localized with previously detected QTL using populations derived from cultivated cucumbers, suggesting that they were under selection during both initial domestication and subsequent improvement. Results from this study also suggested that the wild cucumber is a useful resource for capturing positive transgressive segregation and novel alleles that could be explored in cucumber breeding.     

小菜蛾响应60Co-γ辐照的实验室种群生命表

为明确⁶⁰Co-γ亚不育剂量辐照对小菜蛾Plutella xylostella种群数量动态变化的影响,构建小菜蛾种群动态变化模型,在实验室条件下通过建立小菜蛾生命表获得种群特征参数、个体生理指标,预测亚不育剂量辐照对小菜蛾种群的影响。结果显示,亚不育剂量辐照组的羽化率显著高于完全不育剂量组,与空白对照组无显著差异,各处理羽化率无性别差异,亚不育剂量辐照组F1代孵化率显著低于空白对照组。亚不育剂量辐照组种群各阶段发育历期、存活率及繁殖力生命指标低于空白对照组。小菜蛾亚不育剂量辐照组世代平均历期(19.90 d)、世代净生殖率(2.35)、周限增长率(1.04 d-1)、内禀增长率(0.04 d-1)均低于空白对照组,而种群加倍时间(16.16 d)高于空白对照组。空白对照组世代平均历期、每日单雌产卵量与产卵时间均长于亚不育剂量辐照组。辐照组与对照组种群生命表参数对比,表明亚不育剂量降低了小菜蛾种群世代数和幼虫期的个体存活率,使F1代雌虫个体数减少。     

Genetic characterization of bovine viral diarrhea virus strains in Beijing,China and innate immune responses of peripheral blood mononuclear cells in persistently infected dairy cattle

To acquire epidemiological data on the bovine viral diarrhea virus (BVDV) and identify cattle persistently infected (PI) with this virus, 4,327 samples from Holstein dairy cows were screened over a four-year period in Beijing, China. Eighteen BVD viruses were isolated, 12 from PI cattle. Based on genetic analysis of their 5''-untranslated region (5''-UTR), the 18 isolates were assigned to subgenotype BVDV-1m, 1a, 1d, 1q, and 1b. To investigate the innate immune responses in the peripheral-blood mononuclear cells of PI cattle, the expression of Toll-like receptors (TLRs), RIG-I-like receptors, interferon-α (IFN-α), IFN-β, myxovirus (influenza virus) resistance 1 (MX1), and interferon stimulatory gene 15 (ISG15) was assessed by qPCR. When compared with healthy cattle, the expression of TLR-7, IFN-α, and IFN-β mRNA was downregulated, but the expression of MX1 and ISG-15 mRNA was upregulated in PI cattle. Immunoblotting analysis revealed that the expression of interferon regulatory factor 3 (IRF-3) and IRF-7 was lower in PI cattle than in healthy cattle. Thus, BVDV-1m and 1a are the predominant subgenotypes in the Beijing region, and the strains are highly divergent. Our findings also suggest that the TLR-7/IRF-7 signaling pathway plays a role in evasion of host restriction by BVDV.     

老鸦柿的开发利用

经过５年的试验实践，将野生灌木老鸦柿按其浆果形态的差异区分成３个类型。并对其栽培利用作了较为系统的归纳，将为今后的开发利用提供参考。     

浙江省银杏生产的现状和对策

分析了浙江省银杏资源及其开发利用现状的基础上，揭示了发展银杏的优势和有利条件，以及银杏生产的存在问题，提出了发展银杏生产的对策和措施。     

整地施肥对尾叶桉生长效应的研究

整地施肥试验结果表明，全垦，带垦和穴垦３种整地方式及施肥对尾叶桉造林成活率和保存率均没有显的影响，这３种整地处理加上施肥，对促进尾叶桉生长均能取得理想的效果，５年生材积，施肥是不施肥的９．８倍，全垦和带垦整地虽然在一定程度上促进了林木的生长，但在贫瘠的立地上，加施肥料才能达到速丰产经营目的，整地和施肥间的交互作用不明显，施肥对尾叶桉的促进作用可延至５年以后。     

岷江上游干旱河谷辐射松种子育苗试验研究

经过连续 3年对辐射松种苗特性及育苗技术的试验研究 ,结果表明 :( 1)辐射松种子在 5℃的低温条件下贮藏 2年其发芽率比对照提高 6 4 5 % ;( 2 )用 0 5 %的高锰酸钾液对种子进行消毒处理其幼苗出苗率提高 39 2 % ;( 3)用湿润河砂层积催芽的种子其苗木平均苗高 2 4 0cm ,平均地径 0 33cm ,比对照苗高多 4 8cm ,地径大 0 12cm ;( 4)酸性土上培育的苗木平均苗高比碱性土上培育的苗木平均苗高多 9 8cm ,平均地径大 0 16cm ;( 5 )营养袋规格 10cm× 15cm ,营养土配方为腐殖含量 5 0 %的平均苗高 2 3 3cm ,平均地径 0 3cm ,达到一级苗标准 ;( 6 ) 2月底 3月初播种的苗木比 4月底 5月初播种的苗木苗高多 9 1cm ,平均地径大0 16cm ,出苗率提高 35 6 %。经过综合试验研究 ,场圃出苗率由 40 %提高到 80 %以上 ,每公顷产一年生合格营养袋苗达 12 0万株。