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1.
Ramamoorthy Saraswathy Moturi Muralidhar Das Sanjoy Periyamuthu Kumararaja Sukumaran Suvana Natarajan Lalitha Vinaya Kumar Katneni Arunachalam Nagavel Koyadan Kizhakedath Vijayan 《Aquaculture Research》2019,50(4):1096-1106
The health of the pond environment in shrimp farming is important for sustainable and profitable aquaculture, in which sediment–water interface is the most important influencing area. With this objective, the key parameters of water and sediment at the interface was studied in shrimp ponds with varying salinities and compared with the surface water and soil to understand the variations and the underlying causes. Total ammonia nitrogen and total alkalinity were higher in water at the interface as compared to surface water, whereas pH, nitrite, oxide forms of nitrogen (NO3), phosphorus (PO4) and oxidizing bacteria were observed to be lower. There was no significant difference with respect to macrominerals such as sodium, potassium, calcium and magnesium between water at the interface and surface. These changes are profoundly influenced by the uppermost 1.0‐cm thick sediment layer, where the maximum transition of nutrients takes place with the water column. The outcome of the study highlights the effectiveness of water sampling for metabolites at the interface, which reflects at an early stage any potential deterioration of pond environment, which will help the farmer to initiate timely mitigation measures. 相似文献
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Makesh Marappan Aathithya Rengarajan Venkata Satyanarayana Nallala Krishna Sukumaran Aritra Bera Thirugnamurty Sivaramakrishnan Govindarajan Thiagarajan Muniyandi Kailasam Vijayan Koyadan Kizhakedath 《Journal of fish diseases》2019,42(2):249-256
Viral nervous necrosis (VNN) affects more than 120 species mostly belonging to the order Perciformes. However, none of the brackishwater species belonging to the family Cichlidae under the order Perciformes are reported to be susceptible. Hence, the present experiment was undertaken to study the susceptibility of the brackishwater cichlid, pearlspot, Etroplus suratensis to NNV. Thirty‐day‐old pearlspot larvae were infected with NNV by immersion. Mortality was recorded till 14 days post‐infection, and the infected larvae were subjected to nested RT‐PCR and histology. The virus was isolated from infected larvae using SSN‐1 cells. To study the replication of the virus in vitro, primary cultured brain cells of E. suratensis and IEK cells were infected with NNV. No mortality was observed in any of the control or experimentally infected larvae. However, the experimentally infected larvae were positive for NNV by nested RT‐PCR and the virus was isolated using SSN‐1 cells. Further, the infected pearlspot brain cells and IEK cells showed cytopathic effect at second and third passage of the virus and they were positive for NNV by nested RT‐PCR. Pearlspot is relatively resistant to VNN although the virus could replicate in the larvae and in cell culture. 相似文献
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Path-Coefficient Analysis of Leaf-Anatomical Characters Affecting Frost Injury in Potato 总被引:1,自引:0,他引:1
Correlation and path-coefficient analysis for leaf-anatomical characters affecting frost injury was carried out in twenty eight varieties and accessions belonging to several wild and cultivated tuber-bearing species of Solanum in order to identify selection criteria in breeding for frost resistance in potato Palisade thickness and palisade proportion showed a significant negative correlation with frost injury Path-coefficient analysis revealed that palisade thickness had the highest direct negative effect on frost injury whereas leaf thickness, not conforming to its behaviour as found in simple correlation analysis, emerged ax the next highest factor to have direct positive effect on frost injury. Direct and indirect effects of these factors on frost injury vis a vis their interdependence were found to be in support ot the ‘palisade thickness method’ for screening frost resistant genotypes in potato breeding programmes. 相似文献
5.
Summary The reduction in growth in the dark of leaf dises floated over polyethylene glycol 6000 solution of water potential −0.4 MPa,
relative to the growth of similar leaf dises floated over water for the same period, varied in the range 10–87% in 28 genotypes.
The growth reductions in the genotypes tested were in general agreement with what is known about their drought resistance.
Publication No. 906, Central Potato Research Institute, Simla. 相似文献
6.
The upper incipient lethal temperatures of the freshwater mullet, Rhinomugil corsula, acclimated to 15, 20, 25, 30 and 35°C in fresh water, were 32.4, 34.1, 36.0, 36.2 and 36.5°C respectively, and the corresponding lower lethal temperatures were 10.5, 11.5, 13.2, 15.8 and 19.5°C. The mullet has a total tolerance (area of thermal polygon) of 569°C with an upper and lower thermal tolerance of 253 and 316°C2. Likewise, the total resistance of the mullet was 391°C2, with upper and lower resistance zones of 181 and 210°C respectively. The upper critical temperatures of swimming inhibition of R. corsula (17.2 cm; acclimation 30°C), determined in a swimming tunnel, were 35.2, 34.6 and 34.2 for water current velocities of 38, 62 and 77 cm s?1 respectively. The corresponding lower critical temperatures were 26.2, 27.5 and 28.1°C. These results indicated the stenothermal nature of the mullet by comparison with other fishes, e.g. Tilapia mossambica.In tests on the influence of ambient salinity on thermal resistance, R. corsula survived longest at 7‰ (iso-osmotic salinity). At salinities above and below this point, survival times were shorter at any lethal temperature. In a tentative scheme for quantification of stress due to temperature and salinity at death (after acclimation to 30°C and tested at 37°C), the hypo-osmotic and hyper-osmotic stress were estimated to be 50 and 31% of the thermal stress (100%) respectively. 相似文献
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Krishna Sukumaran Asim K. Pal Narottam P. Sahu Shrinivas Jahageerdar Deepak Khambadakone Dipesh Debnath 《Aquaculture International》2011,19(4):749-763
The present study was conducted to investigate the changes in mineralization and morphology in response to graded levels of
dietary phosphorus in Indian major carp, Catla (Catla catla) fingerlings (av. wt. 4.23 ± 0.87 g). The experimental system constituted of 24 150-L capacity plastic tanks (eight treatments,
three replicates) stocked with 20 fingerlings each. Eight isonitrogenous and isocaloric semi-purified diets (crude protein
35%, crude lipid 8.5%) were formulated with graded levels of phosphorus (0%-control; 0.1; 0.3; 0.5; 0.7; 0.9; 1.1; 1.3; 1.5%)
using KH2PO4. The fish were fed twice daily with the respective feeds for a period of 120 days. The percentage of ash content in whole
body, vertebrae and opercular bones was found to be significantly (P < 0.05) lower in fish fed low levels of dietary phosphorus (T1, T2) in comparison with other treatments. The concentration of phosphorus and calcium was found to be significantly (P < 0.05) lower in whole body, vertebrae and opercula of fish fed low phosphorus diets (T1, T2) relative to treatments with higher levels of dietary phosphorus supplementation. The concentration of magnesium in opercular
and vertebral tissues of T1 group was significantly (P < 0.05) lower than all other groups. The X-ray radiographs did not reveal any skeletal deformities in any of the treatments.
Truss analysis showed absence of significant morphological variations between fish of different treatments. The deficiency
of dietary phosphorus in catla led to significantly lower mineralization of whole body and bone tissues, but the deficiency
was not severe enough to cause skeletal deformities and morphological changes in the fish. 相似文献
9.
John Samuel Godwin Christopher Arunachalam Ganesan Murugesan Natarajapillai Sukumaran 《Aquaculture Research》2010,42(1):129-138
This study reports the results on induced meiotic diploid gynogenesis and female homogametic nature in the Indian catfish, Heteropneustes fossilis. The eggs of H. fossilis were inseminated with conspecific sperm. The sperm suspension was diluted to 1 × 107 sperm mL−1 in Hanks balanced salt solution. Sperm were irradiated under UV light, with the exposure time ranging from 15 to 360 s (7500 ergs mm−2 for 60 s). The genetic inactivation of paternal chromosomes was confirmed by chromosome counting from the larval cells and the larvae also had a characteristic haploid syndrome. A typical ‘Hertwig effect’ in the yield of hatched larvae was observed with doses of UV exposure >75 s (9375 ergs mm2). Larvae resulting from sperm UV irradiated above 120 s (15 000 ergs mm2) were 100% haploids. Application of heat shock to the activated eggs was effective in suppressing the release of the second polar body (meiotic gynogenesis) and resulted in diploid gynogenetic larvae morphologically identical to those of the control. The best yield of diploid gynogens (49.3% with respect to the control) was found to be at 6 min after egg activation and the heat shock at 41 °C for a 1-min duration, at an ambient water temperature of 27 °C. A total of 113 diploid gynogenetic fry from seven different female fish were reared and subjected to sexing. All gynogenetic fish were female in contrast to the control, which had a mean sex ratio of 56.7% females (which was not significantly different from 50% female). From these results, the sex determination mechanism in H. fossilis was presumed to be female homogamety. 相似文献
10.
A feeding trial of 120 days was conducted to study the effect of graded levels of dietary phosphorus on haematology, serum
protein concentrations and HSP70 expression in fingerlings of the Indian major carp, Catla (Catla catla). Eight isonitrogenous and isoenergetic purified diets were formulated to contain graded levels of dietary phosphorus (dP),
i.e., T1, 0.1%; T2, 0.3%; T3, 0.5%; T4, 0.7%; T5, 0.9%; T6, 1.1%; T7, 1.3%; or T8, 1.5%. Four hundred and eighty fish (average weight 4.23 ± 0.016 g) were equally distributed into 24 tanks forming eight
treatments with three replicates each. The fish were fed daily at the rate of 3.5% body weight in two instalments. At the
end of feeding trial fish were sampled to study total RBC and WBC count, haemoglobin, serum lysozyme activity, serum total
protein, albumin (A), globulin (G) concentration and HSP70 expression. Total RBC count, haemoglobin concentration and serum
lysozyme activity did not vary significantly in response to different dietary phosphorus concentrations. Total WBC count was
found to be significantly (P < 0.05) higher in T1 relative to all other treatments. Serum albumin and A/G ratio was found to be significantly lower in fish of T1 and T2 in relation to T7 group (P < 0.05). Serum globulin and total protein levels remained unaffected by variations in dietary phosphorus. HSP70 expression
was observed in T1 group (0.1% dP) in gills and brain tissue, but not in liver and muscle tissues. No HSP70 expression was observed in fish
of T4 (0.7% dP) and T8 (1.5% dP) treatments. These prima facie results suggest that dietary phosphorus had only minor influence on the haemato-biochemical parameters studied; however dietary
phosphorus deficiency caused organ specific induction of HSP70 in catla fingerlings. 相似文献