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Amal Biswas Biswajit K. Biswas Junichi Ito Osamu Takaoka Norishige Yagi Syunsuke Itoh Kenji Takii 《Fisheries Science》2011,77(4):615-621
This study was carried out to investigate whether expensive enzyme-treated fish meal (EFM) can be partially replaced by soybean
meal (SM) or enzyme-treated SM (ESM) without compromising the growth of captive-raised Pacific bluefin tuna (PBT) juveniles.
Five fish diets were tested: EFM (control) and EFM in which 10 or 20% of the EFM was replaced with either ESM or SM (ESM10, ESM20, SM10 and SM20, respectively). The respective diets were provided to 28-day-old PBT juveniles (mean body weight approx. 0.38 g) stocked
randomly in 15-m3 tanks (276 juveniles per tank; two tanks per treatment) for 12 days. The final mean body weight of PBT juveniles on diets
EFM, SM10, and SM20 was significantly higher than that of juveniles on diet ESM20. Although daily feeding rate was significantly higher in the ESM and SM groups, feed efficiency (FE) was significantly higher
in the EFM group; there was no significant difference between juveniles fed diets EFM and SM10. Protein and phosphorus retention efficiency were significantly lower in the ESM20, and ESM20 and EFM groups, respectively. Phosphorus discharge was significantly lower in the SM groups. The results demonstrate that
10% SM (11% EFM replacement) can be included in EFM without compromising the growth performance of PBT juveniles. 相似文献
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Norishige Yotsukura Tadashi Kawai Taizo Motomura Terunobu Ichimura 《Fisheries Science》2001,67(5):857-862
ABSTRACT: Random amplified polymorphic DNA (RAPD) was studied to detect genetic markers for three economically important Japanese laminarian species, Laminaria japonica Areschoug, L. religiosa Miyabe and L. ochotensis Miyabe, which were sampled from their representative localities on the coasts from Tsugaru Straits to the Sea of Japan. DNA templates for RAPD were extracted from lamina using a DNA extraction kit and were purified with glassmilk. Reproducible RAPD markers for these three species were detected using three random primers from a total of 15 tested. In L. japonica and L. religiosa , these RAPD markers were confirmed to be useful for populations from other localities. The three species studied showed high intraspecific and interspecific band sharing index (BSI) values. Like annual typical L. religiosa from other localities, biennial individuals identified as putative L. religiosa from Atsuta could be discriminated from L. japonica but not from L. ochotensis by any of the RAPD genetic markers studied so far. 相似文献
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