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排序方式: 共有199条查询结果,搜索用时 203 毫秒
1.
Sakiko Orui Sakaguchi Shigeru Shimamura Yuichi Shimizu Gen Ogawa Yuichiro Yamada Keiko Shimizu Hiroaki Kasai Hiroshi Kitazato Yoshihiro Fujiwara Katsunori Fujikura Kiyotaka Takishita 《Fisheries Science》2017,83(1):47-56
To identify the stomach contents of marine organisms, morphological observations are commonly conducted. However, the results obtained by this traditional method are frequently biased, as it is difficult to detect partially digested, soft-bodied organisms. To resolve this, recent studies have used DNA-based (DNA barcoding) analyses to examine the diet breadth of marine organisms. Here, we compared the prey richness of juvenile fishes using morphological observations and DNA-based analyses, with a focus on juvenile chum salmon Oncorhynchus keta. A higher number of prey taxa were successfully identified using DNA-based analyses than morphological observations. However, we also noticed several shortcomings of the DNA-based analyses, as reported in other diet-analysis studies that used molecular techniques. For example, the degree of digestion among prey taxa might have resulted in differential sensitivity to DNA detection. Additionally, several prey taxa could not be precisely identified, as the sequence data for some of the targeted organismal groups are unavailable in public gene databases. Remarkably, it is also possible that DNA-based analyses detected secondary prey, and therefore, the richness of prey taxa was likely overestimated. Thus, dietary analyses of juvenile fishes need to be carefully conducted, considering the respective advantages and disadvantages of DNA-based and morphological techniques. 相似文献
2.
Teruo Nakayama Keiko Hatae Midori Kasai Atsushi Ooi 《Journal Of Aquatic Food Product Technology》2017,26(5):578-592
The season for finding the firmest sashimi made from wild Japanese sea bass was investigated. Flesh rigor tension and contraction were measured by a “new” device with isometric (muscle length kept constant) and isotonic (balance beam moves freely) transducers. Both flesh rigor tension and contraction peaks appeared at the same postmortem times, with attainment of ultimate pH and adenosine triphosphate/inosine monophosphate ratio in four seasons. Seasonal seawater temperature correlated negatively to seasonal maximum rigor contraction and positively to seasonal flesh breaking strength at 72 h postmortem. After rigor peak tension attainment, the tension relaxation was much lower than that of other wild fish species, especially in summer. The sea bass muscle resisted structure disruption in rigor isometric tension generation. Wild sea bass sashimi was firmest in breaking strength in summer. 相似文献
3.
Comparison of the canine corneal epithelial cell sheets cultivated from limbal stem cells on canine amniotic membrane,atelocollagen gel,and temperature‐responsive culture dish 下载免费PDF全文
4.
Rieko OKAME Keiko NAKAHARA Noboru MURAKAMI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):815-821
We measured the plasma levels of amino acids at various reproductive stages in female
rats, including the estrous cycle, pregnancy and lactation, and compared the resulting
amino acid profiles using two- or three-dimensional figures. These figures revealed that
the amino acid profiles of pregnant and lactating dams differed considerably from those
during the estrous cycle or in male rats. The plasma levels of individual amino acids were
almost the same between proestrus, estrus, metestrus and diestrus, and their profiles did
not differ significantly. However, the amino acid profiles changed during pregnancy and
lactation in dams. The plasma Ser level decreased significantly in mid and late pregnancy,
whereas Tyr, Gly and His decreased significantly in the late and end stages of pregnancy,
and Trp and Lys significantly decreased and increased at the end of pregnancy,
respectively. Much larger changes in amino acid profiles were observed during lactation,
when the levels of many amino acids increased significantly, and none showed a significant
decrease. Plasma Pro, Ser and Gly levels increased continuously from day 1 until day 15 of
lactation, whereas Asn and Met increased significantly from days 1 and 5 respectively
until the end of lactation. These results suggest that the profiles of plasma amino acids
show characteristic changes according to reproductive stage and that it may be necessary
to consider such differences when performing amino acid-based diagnosis. 相似文献
5.
We examined the influence of nursery soil cover on germination of Thujopsis dolabrata var. hondai. Seeds were sown under seeding bed conditions: (1) nursery soil (andosol) without soil cover, (2) Kanuma pumice without soil
cover, (3) nursery soil with nursery soil cover, and (4) nursery soil with sterilized sand cover. Germination percentages
were compared between these conditions. The germination percentage (8.8%) at 10 weeks after sowing under condition 3 was significantly
lower than under conditions 1, 2, and 4 (56.8%, 52.0%, and 47.2%, respectively). Of the nongerminated seeds under condition
3, 75.0% were decayed and several fungi were isolated from decayed seeds. An inoculation test of isolated fungi Cylindrocarpon tenue, Cylindrocarpon sp., Fusarium oxysporum, and Botrytis sp. to seeds showed that these fungi caused seed decay. Our results indicated that nursery soil cover may not be suitable
for T. dolabrata var. hondai seeds sown in nursery soil because of seed decay by pathogens. 相似文献
6.
7.
Aoshima H Okita Y Hossain SJ Fukue K Mito M Orihara Y Yokoyama T Yamada M Kumagai A Nagaoka Y Uesato S Hara Y 《Journal of agricultural and food chemistry》2005,53(6):1955-1959
Recently, 3-O-octanoyl-(+)-catechin (OC) was synthesized from (+)-catechin (C) by incorporation of an octanoyl chain into C in the light of (-)-epicatechin gallate (ECg) and (-)-epigallocatechin gallate (EGCg), which are the major polyphenols found in green tea and have strong physiological activities. OC was found to inhibit the response of ionotropic gamma-aminobutyric acid (GABA) receptors (GABA(A) receptors) and Na+/glucose cotransporters expressed in Xenopus oocytes in a noncompetitive manner more strongly than does C. OC also induced a nonspecific membrane current and decreased the membrane potential of the oocyte, and thus death of the oocyte occurred even at lower concentrations than that induced by C or EGCg. Although EGCg produced H2O2 in aqueous solution, OC did not. This newly synthesized catechin derivative OC possibly binds to the lipid membrane more strongly than does C, Ecg, or EGCg and as a result perturbs the membrane structure. 相似文献
8.
9.
Keiko Uchida Susumu Takamatsu Sanae Matsuda Kazuhiro So Yukio Sato 《Journal of General Plant Pathology》2009,75(2):92-100
In 2002, a powdery mildew with catenate conidia lacking fibrosin bodies was found on cucumber in a greenhouse in Kanagawa
Prefecture, Japan. Morphological observation revealed that the fungus belongs to Oidium subgenus Reticuloidium, anamorph of the genus Golovinomyces. Molecular phylogenetic analyses of the nucleotide sequences of the rDNA ITS regions and D1/D2 domains of the 28S rDNA indicated
that the fungus belongs to the clade of G. orontii with other Golovinomyces fungi from a wide range of host plants, suggesting that the fungus was newly transported from abroad. Because there has been
no prior report of cucumber powdery mildew caused by Reticuloidium, further research on the physiology, epidemiology, control and resistant cucumber varieties is required. 相似文献
10.