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ABSTRACT: The development of embryos, larvae and juveniles of the Ryukyu-ayu, Plecoglossus altivelisryukyuensis are described based on laboratory-reared specimens.The eggs were spherical, 0.98–1.18 mm (mean 1.06 mm)in diameter with an adherent membrane. The incubation period after fertilizationwas approximately 155 h at a water temperature of 19.7–22.0°C(mean 20.7°C). Newly hatched larvae were 5.0–5.9 mm(mean 5.5 mm) in body length (BL) with 59–62 myotomes.Within 5 days after hatching, the larvae had attained 6.4–8.2 mm(mean 7.8 mm) BL and had completely consumed their yolk.Notochord flexion began at 13.7 mm BL and was completedby 16.3 mm BL. The rudimental dorsal, anal, pelvic andadipose fins appeared at 14.7, 16.3, 21.8 and 21.8 mm BL, respectively.All fin rays reached the same fixed number of adult fish at about28 mm BL. The comb-like teeth began to form at approximately30 mm BL and were fully developed at about 40–50 mm BL.The proportions of P. a. ryukyuensis specimens,which were consistent with adult fish, occurred at approximately40 mm BL. 相似文献
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Syota KAWAGUCHI Ryosuke SAKUMOTO Kiyoshi OKUDA 《The Journal of reproduction and development》2013,59(3):219-224
Luteoprotective mechanisms of luteinizing hormone (LH) involved in the maintenance of
bovine corpus luteum (CL) function have not been completely clarified. Since antioxidant
enzymes are well documented as antiapoptotic factors in the CL of many mammals, we
hypothesized that the luteoprotective action of LH is mediated by stimulating the local
production and action of antioxidant enzymes. To test the above hypothesis, in the present
study, we examined the mechanisms involved in the luteoprotective actions of LH. Cultured
bovine luteal cells obtained from the CL at the mid-luteal stage (days 8–12 of the estrous
cycle) were treated with LH (10 ng/ml), onapristone (OP; a specific progesterone receptor
antagonist, 100 μM) and diethyldithiocarbamate [DETC; an inhibitor of superoxide dismutase
(SOD), 100 μM] for 24 h. LH in combination with or without OP significantly increased the
mRNA and protein expressions of manganese SOD (Mn-SOD) and catalase (CATA) and SOD
activity. While LH alone significantly increased the mRNA and protein
expressions of SOD containing copper and zinc (Cu,Zn-SOD), OP in combination with or
without LH significantly decreased the mRNA and protein expressions of Cu,Zn-SOD. In
addition, Cu,Zn-SOD, Mn-SOD and CATA mRNA expressions were higher at the mid luteal phase
than the other luteal phases. LH in combination with DETC significantly decreased
LH-increased cell viability. The overall results suggest that LH increases cell viability
by LH-increased antioxidant enzymes, resulting in maintenance of CL function during the
luteal phase in cattle. 相似文献
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Yasuo FUSHIMI Mitsuhiro TAKAGI Hiroaki KAWAGUCHI Noriaki MIYOSHI Takeshi TSUKA Eisaburo DEGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(3):337-340
Eosinophilic enteritis (EOE) is a type of inflammatory bowel disease and is characterized
clinically by chronic obstinate diarrhea. Three Japanese Black (JB) fattening cattle (2
males and 1 female) on different cattle farms presented with chronic episodic diarrhea
without fever or dehydration. Soft reddish spherical carneous tissues (1−3 cm) were
occasionally excreted within the diarrheic feces. Administration of antibiotics,
antidiarrheal drugs and vermicides had no therapeutic effect, but dexamethasone improved
the fecal characteristics. The symptoms persisted until the animals were slaughtered at
27–30 months of age. Histopathological examination of the intestines revealed marked
eosinophilic infiltration in the lamina propria and submucosa. From these findings, we
diagnosed these cattle as the first cases of EOE in JB cattle. 相似文献
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A chromatograph of normal rabbit serum shows three major peaks of amylase activity. Material associated with each peak differed in composition of serum protein, electrophoretic distribution of amylolytic activity, optimum pH for activity and Michaelis-Menten constants for starch hydrolysis. These data suggest but do not prove conclusively that normal rabbit serum contains at least three molecular forms of amylase. 相似文献
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