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排序方式: 共有73条查询结果,搜索用时 31 毫秒
1.
大豆杂种优势高优势组合筛选及稳定性分析 总被引:3,自引:0,他引:3
经过1996-2000年5年的研究,共配制杂种优势测定组合199个,通过第一次产量鉴定表明,大豆具有明显的杂种优势,并筛选出F1超高亲,超对照优势率均为正值,且二者平均值大于10%的较强优势组合161个,占全部组合的80.9%,F1超高亲,超对照优势率均大于20%的高优势组合69个占全部组合的34%,并通过第二次产量鉴定进一步筛选出稳定的高优势组合4个,即合9661,9672,9731,97143,此外研究结果还表明,大豆杂种优势具有较好的稳定性,在不同年度,不同地点优势率变化不大,且趋势一致。 相似文献
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或有事项是指过去的交易或事项形成的一种状况,其结果须通过未来不确定事项的发生或不发生予以证实。或有会计事项作为一种特殊的不确定性经济事项,其信息对企业内部管理当局及有关各方经营决策的影响越来越大。针对我国现阶段或有事项的处理披露中存在的问题,一方面应提高会计人员的业务素质,加强对从业会计人员的业务培训;另一方面应充分发挥审计对公司财务信息披露质量的鉴证作用。 相似文献
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A total of 763 faecal samples were collected from western grey kangaroos (Macropus fuliginosus) in Western Australia and screened for the presence of Cryptosporidium by PCR at the 18S ribosomal RNA (rRNA) locus. Samples that were positive at the 18S locus were also amplified at the actin locus. The overall prevalence was 9.3% (71/763). At the 18S rRNA locus, sequences were obtained for 28 of the 71 positives. Sequence analysis identified four species; Cryptosporidium fayeri in seven isolates, Cryptosporidium marcopodum in four isolates, Cryptosporidium xiaoi in six isolates and a novel genotype (kangaroo genotype I) in eleven isolates. Analysis at the actin locus confirmed the genetic distinctness of the novel genotype. The results of the present study indicate that in addition to C. fayeri and C. marcopodum, kangaroos may be capable of being infected with a wider range of Cryptosporidium species and genotypes including livestock species such as C. xiaoi. The novel genotype identified in the kangaroos most likely represents a cryptic species that requires further analyses to confirm its species status. 相似文献
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Zhiqiang Huang Meng Yu Shuang Tong Kun Jia Rongchang Liu Heng Wang Shoujun Li Zhangyong Ning 《Journal of veterinary science (Suw?n-si, Korea)》2014,15(2):173-177
Activation of the innate immune system requires recognition of pathogen-associated molecular patterns, such as NOD-like receptors. The NOD-like receptor protein 3 (NLRP3) inflammasome is involved in induction of the pro-inflammatory cytokine, IL-1β, and subsequent inflammatory responses. NLRP3 inflammasome plays important roles in the inflammatory and innate immune responses associated with autoimmune/inflammatory syndrome. However, analysis of the tissue distribution and expression profiles in BALB/c mice is still incomplete. In this study, we investigated the tissue distribution and expression pattern of NLRP3 in BALB/c mice to further elucidate its function in innate immunity in this commonly used laboratory animal model. NLRP3 mRNA expression levels and tissue distribution of the protein were investigated by real-time quantitative PCR and immunohistochemical analyses, respectively. NLRP3 mRNA expression was higher in the kidney and inguinal lymph nodes than in other tissues. Cytoplasmic expression of NLRP3 was detected in the epithelial reticular cells of the spleen and thymus, lymphocytes in the inguinal lymph nodes, cardiac muscle cells, cerebral cortex neurons, alveolar macrophages, renal tubule cells and liver sinusoidal endothelial cells. The results of this study will assist investigators in interpreting site-specific functions and roles of NLRP3 in inflammatory responses. 相似文献
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1990~1995年间,首次对中国大陆30个省(市)番茄TMV进行了基因型株系分化的研究,在695个TMV纯化分离物中鉴定出了TMV-0、1、2和1.2株系,其中0株系分布最广,是19个省(市)的优势株系,占当地TMV分离物的50%以上;1株系分布也很广泛,是11个省(市)的优势株系,占40%以上;2株系较少,虽然17个省(市)有分布,但占4.5%~30.6%;1.2株系最少,仅在6个省(市)中发现,占4.0%~11.1%。这一研究结果为我国各省(市)番茄抗TMV育种确定了主攻目标。 相似文献
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Muhid A Robertson I Ng J Yang R Ryan U 《Veterinary journal (London, England : 1997)》2012,191(1):135-137
Two hundred and forty calf faecal samples from 16 Malaysian farms were screened by PCR for Giardia spp. The overall prevalence was 12.5% and the overall farm prevalence was 68.8% (11/16 farms). The prevalence in pre-weaned and weaned calves was 16.7% and 8.3%, respectively. Sequence analysis of 25 isolates identified all as G. duodenalis assemblage E. Management factors associated with an increased risk of infection with Giardia spp. included keeping weaned calves in pens with sand floors and calf age. Keeping pre-weaned calves in pens with concrete floors and calving in single cow calving areas decreased the risk. 相似文献
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马铃薯纺锤块茎类病毒的检测和防治 总被引:5,自引:1,他引:5
马铃薯纺锤块茎类病毒病(potato spindle tuber viroid,PSTVd)是一种严重为害马铃薯生产的病害,降低产量20%—30%。防治的主要措施是应用无类病毒的种薯。由于目前还没有脱掉类病毒的有效措施,只能从未被饱和侵染的群体中鉴定筛选出未被侵染的个体,再脱掉其它病毒,作为核心繁殖材料。1987年以来,利用自制的电泳设备,以往复聚丙烯酰胺凝胶电泳法(return-polyacrylamide gel electrophoresis,R-PAGE)检测类病毒,筛选出未感病的个体,再用茎尖组织培养法脱掉其它病毒。经用马铃薯卷叶病毒等8种病毒酶标抗体鉴定筛选,获得既无类病毒也无主要马铃薯病毒的克新1、2、3和4号等主栽马铃薯品种的核心种。并已提供给省内外的良种场繁殖推广。1989和1990年抽样检测克山良种场繁殖的原种、一级和二级良种,未检测到类病毒。 相似文献
10.
Fei Zhou Liancheng Cui Zhiqiang Huang Shuang Tong Meng Yu Rongchang Liu Zhangyong Ning 《Veterinary research communications》2013,37(4):285-291
In chicken, ST3 beta-galactoside alpha-2, 3-sialyltransferase III (ST3GAL III) is one of the key enzymes participating in the biosynthesis of avian influenza virus receptors. Knowledge about ST3Gal III could increase our understanding of its function in the occurrence and development of avian influenza. To date, no detailed data have been published about the expression pattern and histological distribution of ST3Gal III in chicken. This paper presents data on the nucleotide sequence, mRNA expression pattern and histological distribution of ST3Gal III protein in yellow chicken for the first time. The results show that the nucleotide homology of ST3Gal III among yellow chicken and turkey, chicken, cattle, humans, swine, mouse, rat and chimpanzee was 98 %, 92 %, 75 %, 59 %, 70 %, 76 % and 75 %. The relative expression level of ST3Gal III in the heart, kidney and brain of yellow chicken was significantly higher than in other tissues examined for mRNA level (P?<?0.05). The histological distribution of ST3Gal III in the heart, liver, spleen, lung, thymus and bursa of Fabricius was determined by immunohistochemical staining using rabbit anti-chicken ST3Gal III IgG prepared by us. Interestingly, the epithelial reticular cells in the immune organs proved to be positive, which may suggest that these cells are important immune cells playing a role in influenza virus infection. The results of this study provide basic data for further research on the functions of ST3Gal III in chicken. 相似文献