云南烟草丛枝症病害研究 V传染途径

在隔离环境中,按植物病毒学的标准程序,测试云南烟草丛枝症病害的传染途径,结果表明：云南烟草丛枝症病害具有广泛的传染途径,可以通过机械传染（嫁接、摩擦）和介体传染（菟丝子、蚜虫、烟盲蝽、叶蝉）等方式传播．     

蜂胶防治大田烟草病害效果试验

用蜂胶乙醇提取液对大田栽培的烟株进行病害防治的试验 ,结果表明 4种不同浓度的蜂胶对烟草病害均有防治作用 ,以原液稀释 50 0倍、 80 0倍防治效果最好。     

尼龙袋法评定鹿常用饲料的营养价值

用３头装有永久性瘤胃瘘管的成年公梅花鹿，采用尼龙袋法，评定了４９种７３个样品鹿常用饲料的干物质有效降解率（ＤＭＤ，下同），蛋白质有效降解率（ＣＰＤ，下同），有机物有效降解率（ＯＭＤ，下同）。结果表明，尼龙袋法能较好地评定梅花鹿饲料的ＤＭＤ、ＣＰＤ、ＯＭＤ和反映饲料品质差异；同类不同种饲料及同种饲料因收获期不同、处理不同、部位不同其ＤＭＤ、ＣＰＤ、ＯＭＤ差异较大；同种不同产地饲料间的ＤＭＤ、ＣＰＤ亦有差别。     

免疫调节剂与抗球虫药联合应用的抗球虫效果

为探讨免疫调节剂对鸡球虫病的免疫反应调节作用,设计并进行了免疫调节剂与抗球虫药合用的效果的研究。试验结果以抗球虫指数的测定来判定抗球虫药与免疫调节剂合用的效果。结果表明,免疫调节剂与抗球虫药合用状态下,抗球虫指数检测显示免疫调节剂卡介苗和黄芪多糖可明显提高抗球虫指数、改善球虫感染鸡的临床症、提高增重速度等,为控制球虫病提供了新的途径。     

化药防治羊硬蜱病试验

将1150只感染有草原革蜱和森林革蜱的绵羊分为3个组,分别为驱蜱项圈组（400只）、灭蜱灵组（550只）和对照组（200只）。项圈组在治疗后0、7、15、28、45、60、75、95天,羊只平均染蜱数分别为10.3、0.9、0.7、0.3、0.4、0.5、0.5、0.1只;而对照组分别为10.2、9.2、13.6、10、9.2、6.4、8.8、4.2只。灭蜱灵组在治疗后0、18、33、48、68天,羊只平均染蜱数分别为9.8、0.5、0.7、0.5、0.1只,而对照组分别为10、9.2、6.4、8.8、4.2只,表明两个防治组保护效果均较好。     

几种旋毛虫抗原PAPS诊断液的效果比较试验

将几种旋毛虫抗原以同一浓度（0.6mg/mL）与PAPS微球交联,制成诊断试剂,进行旋毛虫阳性血清检测效果比较试验。结果显示,成虫、成虫ES、新生幼虫、肌幼虫B峰4川抗原均出现非特异性反应,而肌幼虫、肌幼虫ES、肌幼虫A峰抗原具有很高的敏感性,三者效价均达1:640。将此3种抗原所制得的诊断试剂对几种寄生虫阳性血清进行交叉试验,显示肌幼虫ES抗原对血吸虫、锥虫、弓形虫、肺吸虫、牛肝片吸虫、羊肝片吸虫阳性血清均不出现交叉反应,而肌幼虫A峰抗原、肌幼虫抗原对牛、羊肝片吸虫均出现交叉反应,对其他血清则不出现。表明肌幼虫ES抗原效果最佳,特异性最好,有很高的应用价值。     

Real-time observation of bimodal proton transfer in acid-base pairs in water

The neutralization reaction between an acid and a base in water, triggered after optical excitation, was studied by femtosecond vibrational spectroscopy. Bimodal dynamics were observed. In hydrogen-bonded acid-base complexes, the proton transfer proceeds extremely fast (within 150 femtoseconds). In encounter pairs formed by diffusion of uncomplexed photoacid and base molecules, the reaction upon contact was an order of magnitude slower, in agreement with earlier reported values. These results call for a refinement of the traditional Eigen-Weller picture of acid-base reactions: A three-stage model is introduced to account for all observed dynamics.     

M_(99-1)引诱剂诱捕松墨天牛等松甲虫的研究

报道了松墨天牛成虫活动期间 ,在马尾松 -栎类次生混交林、马尾松 -湿地松人工林和黄山松天然林 3种不同林分中 ,应用 M99-1引诱剂诱捕钻蛀性松甲虫种类、主要钻蛀性松甲虫成虫种群数量及其比率和不同诱捕期松墨天牛雌成虫孕卵量及其比率的情况。研究结果显示 ,M99-1引诱剂诱获鞘翅目害虫达 2 6种 ,其中钻蛀性松甲虫达 14种 ,占诱获种类的 5 3.8%。马尾松角胫象、松墨天牛种群数量比率最高 ,分别达 5 7.3%～ 69.8%和 2 7.2 %～ 2 9.3%。 3种不同松林林分中 ,短角幽天牛、褐幽天牛、薄翅锯天牛等其它主要钻蛀性害虫的数量比率也较高。不同诱捕期松墨天牛雌成虫平均孕卵量及其比率 ,以活动前期 (6月下旬前 )为最高 ,分别达 16.9粒和 4 1.9%。 M99-1引诱剂诱捕是监测松林钻蛀性松甲虫种群动态和降低其虫口密度的重要方法     

四种剂型阿维菌素对肉用犬疥螨病的疗效试验

四种剂型阿维菌素 (粉剂、片剂、注射剂、浇注剂 )以 0 .2 mg/kg剂量治疗肉用犬疥螨病 ,间隔 7天重复用药 1次 ,治愈率均为 1 0 0 %,且安全无副作用。     

An Inter‐Subspecies Cloned Buffalo (Bubalus bubalis) Obtained by Transferring of Cryopreserved Embryos via Somatic Cell Nuclear Transfer

The aim of this study was to explore the feasibility of cryopreservation of inter‐subspecies cloned embryos in buffalo. In our experiment, river buffalo ear fibroblast nucleus was fused into swamp buffalo oocyte cytoplasm. The blastocyst formation rate for nuclear transfer of freshly thawed cells was not different from those of growing cells, confluent or serum‐starved cells. A total of 122 cloned blastocysts derived from cryopreserved fibroblasts were cryopreserved and thawed, 37 were survived, the cryosurvival rate was 30.3%. The survived blastocysts were transferred into 15 recipient buffalos. Five of the recipients established pregnancy, but four of them aborted on day 53, 59, 145 and 179 of gestation respectively. One cross‐bred buffalo (Murrah × Swamp buffalo (2n = 49) received three embryos delivered a 40.5 kg female calf by natural delivery on day 320 of gestation. Up to now (13‐month old), the cloned calf has been growing well with no abnormity observed. These results demonstrated that cryopreservation of inter‐subspecies cloned embryos is feasible to produce buffalo offspring.