设施栽培黄瓜全生育期主要病虫害绿色防控集成技术

为指导设施栽培黄瓜的有序安全生产,利用实施上海市科委、上海市农委的项目,总结集成了黄瓜主要病虫害绿色防控技术.其集成特点:注重设施与种子消毒,培育壮苗,以生态调控、预防为主,在农药选用上考虑多种病虫兼治.通过集成技术规程的实施,可保证黄瓜整个生长期基本免受主要病虫的为害,比常规防治相对减少农药使用量50％左右(折合原药),黄瓜单产平均增加40％～50％.     

兽医实验室安全操作规范

正1 入门前的准备1.1 实验室处于封闭状态。实验操作进行中时,要限制非实验人员的进出,如有特殊情况必须经实验室相关负责人同意后,方可进入。1.2 张贴国际通用的生物危险标识。1.3 只有经过培训和许可的人员方能进入实验室。2 岗前培训2.1 实验、研究人员、外来工作人员、学生使用实验室前必须参加生物安全培训。2.2 要接受有关潜在危险知识的培训,掌握预防暴露以及暴露后处理程序。2.3 每年要接受一次最     

东阳市省级农村综合改革集成示范区建设成效及启示

为更好地发挥农村综合改革在实施乡村振兴战略中的示范引领作用,浙江省财政厅聚焦聚力城乡发展不平衡不充分问题,组织开展了农村综合改革集成示范建设。经过对嘉善、德清、临安、东阳、缙云等县(市、区)深入调研,以东阳市花园村农村综合改革集成示范区建设为着重点,剖析产权制度改革、乡村产业振兴、宜居环境打造、集体经济壮大、农民增收和乡村治理等六大方面的主要成效、典型经验和启示,为全省高质量发展的乡村振兴提供可推广复制的"花园样本"。     

鸡白痢沙门菌pagC基因缺失株菌蜕疫苗的制备和免疫保护效果评价

为了在鸡白痢沙门菌pagC基因缺失突变株的基础上制备可用于鉴别诊断的菌蜕疫苗并评价其免疫保护效果,本研究利用细胞穿膜肽MAP制备ΔPagC菌蜕,透射电镜和荧光显微镜观察菌蜕形态和核酸残留情况;将ΔPagC菌蜕与佐剂ISA206混合乳化制备菌蜕疫苗并进行无菌检测;基于ICR小鼠评价菌蜕疫苗对鸡白痢沙门菌CVCC1800、肠炎沙门菌ATCC19585和鼠伤寒沙门菌CVCC542感染的交叉保护效果;结合基于PagC蛋白的沙门菌血清学检测方法评价pagC基因缺失株菌蜕疫苗的鉴别诊断效果。结果显示,菌蜕制备最佳条件为用终浓度为50μmol/L MAP溶液处理OD_{600 nm}=0.3的菌液1 h以上,灭活效果可达99.00%;菌蜕保持基本细胞形态的同时内容物大量释出,核酸残留水平较低;菌蜕疫苗针对上述3种血清型沙门菌的免疫保护率分别为25%、100%和100%;菌蜕疫苗与基于PagC蛋白的沙门菌血清学检测方法联用可在一定程度上起到鉴别诊断作用。结果表明,制备所得菌蜕疫苗在保护小鼠的同时,能够在一定程度上区分人工免疫和自然感染,为沙门菌的净化工作提供帮助。     

Effects of Bacillus amylolyticus on Rumen Dry Matter Digestibility,Fermentation Parameters and Metabolites of Dairy Cows北大核心CSCD

This experiment was conducted to study the effects of Bacillus amylolyticus on rumen dry matter digestibility, fermentation parameters and metabolites of dairy cows. Experiment 1: six dairy cows with permanent fistula and had the similar parity (2 to 3 fetuses), body weight [(662 ±57) kg], lactation days [(160 ± 22) d] and milk yield [(36.1 ±3.8) kg / d], were randomly divided into 3 groups with 2 cows per group. Using 3 ×3 Latin square design, each group was extra fed 0 (control group), 5 ×109 and 5 ×1010 CFU / d Bacillus amylolyticus, the trial period was 3 periods, 13 days for each period, including 10 days for the pre-trial period and 3 days for the trial period. At the beginning of the trial period, the rumen dry matter digestibility of cows was measured by nylon bag method. Experiment 2: thirty dairy cows with similar parity [(2.5 ±0.3) fetuses], initial body weight [(559.2 ± 7. 4) kg], milk yield [(35. 2 ± 1. 5) kg / d] and lactation days [(99 ± 22) d] were randomly divided into three groups with 10 cows in each group. Cows in the 3 groups were extra fed with 0 (control group), 5 ×109 and 5 ×1010 CFU / d Bacillus amylolyticus, respectively. The pre-trial period was 10 days and the trial period was 42 days. The rumen fluid was collected on the last day of the trial period, and the rumen fermentation parameters and metabolites were determined. The results showed as follows: compared with the control group, 1) supplementary feeding of 5 × 1010 CFU / d Bacillus amylolyticus could significantly improve the rumen dry matter digestibility of dairy cows at 12, 24, 36, 48 and 72 h (P <0.05), supplementary feeding of 5 ×109 CFU / d Bacillus amylolyticus could significantly improve the rumen dry matter digestibility of dairy cows at 36 and 48 h (P <0.05); 2) supplementary feeding of 5 ×109 CFU / d Bacillus amylolyticus could significantly increase the contents of isobutyric acid, butyric acid, isovaleric acid, valeric acid, total volatile fatty acid and microbial protein in the rumen of dairy cows (P < 0. 05), supplementary feeding of 5 × 1010 CFU / d Bacillus amylolyticus could significantly increase the contents of isobutyric acid and isovaleric acid in the rumen of dairy cows and significantly reduce the rumen acetic acid / propionic acid (P < 0. 05), supplementary feeding of 5 ×109 and 5 ×1010 Bacillus amylolyticus had no effects on rumen pH and ammonia nitrogen content (P >0.05); 3) eleven metabolic pathways related to differential metabolites were detected, and ascorbic acid and alginate metabolism were significantly enriched (P < 0. 05) . In conclusion, Bacillus amylolyticus can increase the rumen dry matter digestibility, and improve the rumen fermentation function of dairy cows by affecting the content of rumen metabolites. By comprehensive analysis, supplementary feeding of 5 × 109 CFU / d Bacillus amylolyticus can achieve better effect. © 2023 Authors. All rights reserved.     

枫杨研究进展

枫杨是我国长江中下游地区广泛分布的重要优良乡土树种和速生丰产造林树种.笔者从种源、育苗造林、群落分布、材性、淹水、灭螺等方面总结了枫杨研究进展情况,对枫杨培育和综合利用以及开展枫杨的速生丰产研究具有重要的理论价值和实际意义.     

鸡白痢沙门菌pagC缺失株的构建及菌蜕制备

为了开发具有鉴别诊断功能的鸡白痢沙门菌疫苗,通过λ-Red同源重组敲除鸡白痢沙门菌CVCC1800的pagC基因,成功获得ΔpagC菌株;合成细胞穿透肽(cell penetrating peptide,CPP)制备菌蜕并对处理条件进行了优化。结果显示:作用浓度为50μmol/L,ΔpagC菌液OD_(600 nm)=0.4左右时CPP灭活效果最好。本研究制备的基于ΔpagC鸡白痢沙门菌菌蜕,在保留抗原性的同时为鉴别诊断提供了靶点,为后续开发针对鸡白痢沙门菌的鉴别诊断菌蜕疫苗奠定了基础。     

浅析搜爆犬在冰雪环境下作业的训练及应用

正举世闻名的第24届冬季奥林匹克运动会将在2022年2月在我国北京市和河北省张家口市举行,这也是我国历史上第一次举办冬奥会。共设7个大项,15个分项,109个小项,总体分为冰上和雪上项目,伴随我国的冰雪旅游开发呈现井喷式的发展态势,随之而来的是运动场和人流密集区的安保警卫问题。例如,哈尔滨市仅2016～2018年度的冰雪季节,冰雪类大型活动规模高达2.7亿人次。同时,由于     

零风险食品诉求不能否认转基因食品的安全性

为了证明转基因食品的安全性,反驳转基因不是"天然的"、转基因食品无法保证100%安全、转基因食品不能吃等错误言论。通过分析政府对食品的安全管理与食品安全间的关系、消费者对食品安全的认知与其食品消费行为间的关系,论证了转基因食品是安全的;并进一步通过分析"纯天然"食品和"零风险"食品的不存在,再次证明转基因食品的安全性。说明过激的食品安全需求不能否定转基因制品的安全性。     

百合花色及相关育种研究进展

花色是百合属植物重要的观赏性状之一。目前世界上对百合种质创新研究大力开发,但在花色创新方面进入瓶颈期。我国百合种质资源丰富,但种质创新发展相对缓慢。花色创新更是在起步阶段。该研究总结了百合花色的相关研究成果,并对百合花色育种进行了综述和展望,以期为进一步开展百合的花色创新提供参考依据。