山东莱州发现三倍体薤白(英文)

[Objective] The study was to analyze the karyotype of Allium macrostemon Bunge in Laizhou City of Shandong Province. [Method] The root tip of A.macrostemon was pretreated with 8-hydroxyquinoline solution, fixed, dissociated and stained for preparing the glass slide to reveal the chromosome number via the microscopic examination; the sparse cells with good chromosome morphology were photographed under microscope. [Result] Allium macrostemon Bunge in Laizhou City introduced in this study was triploid; its somatic chromosome number was 24 and karyotype formula was K(2n)=3x=24m(2SAT)+1Bs, thus the karyotype belongs to 1A type. One of the chromosome No. 3 contained satellite, and chromosome deletion may be existed in one of the chromosome No. 5. In addition, B chromosome was observed in some cells. [Conclusion] This introduction of triploid A.macrostemon found in China was the first time.     

山东莱州发现三倍体薤白

[目的]对山东莱州居群的薤白进行染色体核型分析。[方法]取薤白根尖,用8-羟基喹啉溶液预处理后,固定、解离、染色后制片,镜检并统计染色体数目,然后选用染色体形态好且分散的细胞,在显微镜下拍照分析。[结果]莱州居群薤白为三倍体,体细胞染色体数目为24,核型公式为K（2n）=3x=24 m（1SAT）＋1Bs,属于1A型。3号染色体中的1条具有随体,5号染色体中的1条可能出现缺失现象。另外,部分细胞内还发现B染色体的存在。[结论]三倍体薤白的存在国内尚属首次发现。     

山东莱州发现三倍体薤白

1材料与方法 供试薤白取自山东省莱州市郊外。取薤白的根尖用0．002mol／L 8-羟基喹啉溶液预处理4h,卡诺氏液在冰箱中固定24h,以1mol／L的盐酸60℃解离6～9min,再用改良苯酚品红染色液染色后制片。镜检并统计染色体数目,然后选用染色体形态好且分散的细胞,在配有Olympus DV70数码摄像头的多功能系统显微镜（Olympus BX50）下拍照分析。核型分析按照李懋学、陈瑞阳报道的标准进行,核型分类参照Stebbins GL的方法划分。     

烟台绵枣儿的核型研究

[目的]研究烟台绵枣儿的染色体数目和核型情况。[方法]取绵枣儿根尖,用8-羟基喹啉溶液预处理后,固定、解离、染色后制片,镜检并统计染色体数目。然后选用染色体形态好且分散的细胞,在显微镜下拍照分析。[结果]烟台绵枣儿的体细胞染色体数目为2n=16+1Bs,核型公式为K(2n)=2x=16+1Bs=6m+4sm(2SAT)+4st+2t+1Bs,核型分类为"3B"型。[结论]对烟台绵枣儿从核型比较、细胞型划分、种的进化等方面进行探讨,为细胞遗传学、进化遗传学、现代分类学、遗传育种等提供研究依据。