新基因RSTA14-44在大肠杆菌中的表达及抗体制备

RSTA14-44 基因是中国协和医科大分子生物学试验室在研究中获得的一个新基因,全长 1124 bp,可读编码框自 100bp~684 bp,编码 194 个氨基酸,预测其编码蛋白质分子量为 21.8 KDa。构建了包含 RSTA14-44 cDNA 全部编码框的 pET30a(+)-14 表达载体质粒,经过聚丙烯酰氨凝胶电泳纯化,免疫家兔制备了抗 RSTA14-44 抗体。Western免疫印迹分析表明,该抗体具有较高的反应性和特异性,适合用作 RSTA14-44 蛋白的进一步研究。     

不同高度的防虫网隔离对水稻花粉飘移的影响

以籼稻恢复系R128和籼稻不育系龙特浦A为材料,研究不同高度的防虫网隔离对水稻(Oryza sativa L.)花粉飘移的影响。结果表明,2.0、2.5、3.0 m高防虫网隔离均能影响花粉漂移的频率,各处理同方向各处间的漂移频率随着距离的增大越来越小,差异越来越不明显;花粉漂移频率与风向和风速大小密切相关,即下风口的漂移频率高于上风口的漂移频率,防虫网隔离后漂移频率减少幅度较大;生产杂交种可以使用2.5 m和3.0 m高防虫网作为隔离屏障,不育系扩繁种子使用3.0 m高防虫网作为隔离屏障才符合种子的纯度要求。     

植物修复金属污染土壤上丛枝菌根真菌与植物之间的交互作用

Metal contamination in the environment is a global concern due to its high toxicity to living organisms and its worldwide distribution.The principal goal of this review is to examine the current strategies and technologies for the remediation of metalcontaminated soils by metal-accumulating plants and assess the roles of arbuscular mycorrhizal(AM)fungi in remediation of soils under hyperaccumulator or non-accumulator plants.The use of plants to remove metals from the environment or reduce the toxicity,known as phytoremediation,is an environmentally sustainable and low cost remediation technology.The mechanisms of the use of hyperaccumulator plants for phytoremediation included solubilization of the metal in the soil matrix,the plant uptake of the metal,detoxification/chelation and sequestration,and volatilization.Recently,some ecologists have found that phytoremediation with the aids of mycorrhizae can enhance efciency in the removal of toxic metals.AM fungi can facilitate the survival of their host plants growing on metal-contaminated land by enhancing their nutrient acquisition,protecting them from the metal toxicity,absorbing metals,and also enhancing phytostabilization and phytoextraction.Such information may be useful for developing phytoremediation program at metal-contaminated sites.