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1.
A change in the European Union energy policy has markedly promoted the expansion of biogas production.Consequently,large amounts of nutrient-rich residues are being used as organic fertilizers.In this study,a pot experiment was conducted to simulate the high-risk situation of enhanced greenhouse gas (GHG) emissions following organic fertilizer application in energy maize cultivation.We hypothesized that cattle slurry application enhanced CO2 and N2O fluxes compared to biogas digestate because of the overall higher carbon (C) and nitrogen (N) input,and that higher levels of CO2 and N2O emissions could be expected by increasing soil organic C (SOC) and N contents.Biogas digestate and cattle slurry,at a rate of 150 kg NH4+-N ha-1,were incorporated into 3 soil types with low,medium,and high SOC contents (Cambisol,Mollic Gleysol,and Sapric Histosol,termed Clow,Cmedium,and Chigh,respectively).The GHG exchange (CO2,CH4,and N2O) was measured on 5 replicates over a period of 22 d using the closed chamber technique.The application of cattle slurry resulted in significantly higher CO2 and N2O fluxes compared to the application of biogas digestate.No differences were observed in CH4 exchange,which was close to zero for all treatments.Significantly higher CO2 emissions were observed in Chigh compared to the other two soil types,whereas the highest N2O emissions were observed in Cmedium.Thus,the results demonstrate the importance of soil type-adapted fertilization with respect to changing soil physical and environmental conditions. 相似文献
2.
Kazuya KUSHIDA Urs GIGER Toshihiko TSUTSUI Megumi INABA Yoshio KONNO Kureha HAYASHI Kana NOGUCHI Akira YABUKI Keijiro MIZUKAMI Moeko KOHYAMA Yasuyuki ENDO Osamu YAMATO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(6):743-746
Erythrocyte pyruvate kinase (PK) deficiency is an inherited glycolytic erythroenzymopathy
caused by mutations of the PKLR gene. A causative mutation of the feline
PKLR gene was originally identified in Abyssinian and Somali cats in
the U.S.A. In the present study, a TaqMan probe-based real-time PCR genotyping assay was
developed and evaluated for rapid genotyping and large-scale screening for this mutation.
Furthermore, a genotyping survey was carried out in a population of four popular purebred
cats in Japan to determine the current mutant allele frequency. The assay clearly
displayed all genotypes of feline PK deficiency, indicating its suitability for
large-scale survey as well as diagnosis. The survey demonstrated that the mutant allele
frequency in Abyssinian and Somali cats was high enough to warrant measures to control and
prevent the disease. The mutant allele frequency was relatively low in Bengal and American
Shorthair cats; however, the testing should still be carried out to prevent the spread of
the disease. In addition, PK deficiency should always be considered in the differential
diagnosis of anemia in purebred cats in Japan as well as worldwide. 相似文献
3.
Kormann Urs G. Scherber Christoph Tscharntke Teja Batáry Péter Rösch Verena 《Landscape Ecology》2019,34(5):1045-1056
Landscape Ecology - Global change pressures (GCPs) imperil species and associated ecosystem functions, but studies investigating interactions of landscape-scale pressures remain scarce. Loss of... 相似文献
4.
In Central Europe vast wetland areas have been converted into agricultural land over the past few centuries. Long-term spatially
explicit reconstructions of wetland cover changes at regional scale are rare but such information is vital for setting appropriate
wetland conservation and restoration goals. In this study wetland cover change over the past 150 years was analyzed for the
Canton Zurich (Switzerland) using information from historical and current topographical maps. Mapping instructions changed
significantly over time, i.e., wetlands were mapped more conservatively on older maps. Therefore a technique was developed
to account for changes in mapping instructions and to reconstruct a series of comparable maps spanning 1850–2000. Wetland
cover dramatically decreased from 13,759 ha in 1850 (more than 8% of the total study area) to 1,233 ha in 2000 (less than
1%). Largest loss is observed for the first half of the twentieth century when more than 50% of the total wetland loss occurred.
In 1850, almost all wetland patches were connected in two large networks defined by a 500 m buffer around all wetland patches
to account for typical dispersal distances of wetland animals. Despite extensive wetland loss, this networks remained largely
intact until 1950, but then collapsed into many medium and small networks consisting of only few wetland patches. In addition
to the direct loss of wetland habitats increased habitat fragmentation is limiting metapopulation dynamics and hindering genetic
exchange between populations. Amphibians and other wetland animals are particularly prone to habitat fragmentation because
of their limited migration abilities. This may lead to time-delayed extinction in the future because current species occurrence
might rather reflect historical than current wetland cover and habitat configuration. Future restoration efforts should focus
on reestablishing connectivity between remaining smaller wetland networks. 相似文献
5.
A new acylated quercetin glycoside, quercetin 3-O-(2-t-p-coumaroyl)-beta-D-glucopyranosyl-(1-->2)-beta-D-glucopyranoside-7-O-beta-D-glucopyranoside (1), together with the known 3-(3,4-dihydroxyphenyl)lactic acid (2) were isolated from Ranunculus lanuginosus leaves and identified on the basis of detailed spectral analysis, including 2D-NMR spectrometry and ESI-MS. 相似文献
6.
7.
Mohammad I. Khalil Muhammad S. Rahman Urs Schmidhalter Hans‐Werner Olfs 《植物养料与土壤学杂志》2007,170(2):210-218
A 90‐day laboratory incubation study was carried out using six contrasting subtropical soils (calcareous, peat, saline, noncalcareous, terrace, and acid sulfate) from Bangladesh. A control treatment without nitrogen (N) application was compared with treatments where urea, ammonium sulfate (AS), and ammonium nitrate (AN) were applied at a rate of 100 mg N (kg soil)–1. To study the effect of N fertilizers on soil carbon (C) turnover, the CO2‐C flux was determined at nine sampling dates during the incubation, and the total loss of soil carbon (TC) was calculated. Nitrogen turnover was characterized by measuring net nitrogen mineralization (NNM) and net nitrification (NN). Simple and stepwise multiple regressions were calculated between CO2‐C flux, TC, NNM, and NN on the one hand and selected soil properties (organic C, total N, C : N ratio, CEC, pH, clay and sand content) on the other hand. In general, CO2‐C fluxes were clearly higher during the first 2 weeks of the incubation compared to the later phases. Soils with high pH and/or indigenous C displayed the highest CO2‐C flux. However, soils having low C levels (i.e., calcareous and terrace soils) displayed a large relative TC loss (up to 22.3%) and the added N–induced TC loss from these soils reached a maximum of 10.6%. Loss of TC differed depending on the N treatments (urea > AS > AN >> control). Significantly higher NNM was found in the acidic soils (terrace and acid sulfate). On average, NNM after urea application was higher than for AS and AN (80.3 vs. 71.9 and 70.9 N (kg soil)–1, respectively). However, specific interactions between N‐fertilizer form and soil type have to be taken into consideration. High pH soils displayed larger NN (75.9–98.1 mg N (kg soil)–1) than low pH soils. Averaged over the six soils, NN after application of urea and AS (83.3 and 82.2 mg N (kg soil)–1, respectively) was significantly higher than after application of AN (60.6 mg N (kg soil)–1). Significant relationships were found between total CO2 flux and certain soil properties (organic C, total N, CEC, clay and sand content). The most important soil property for NNM as well as NN was soil pH, showing a correlation coefficient of –0.33** and 0.45***, respectively. The results indicate that application of urea to acidic soils and AS to high‐pH soils could be an effective measure to improve the availability of added N for crop uptake. 相似文献
8.
Daub Matthias Hakl Ulrike Molendijk Leendert PG Schomaker Corrie Been Thomas H van Beers Thea G Jivishova Sevda Jivishov Emil Keusgen Michael Bluemel Roman Fischer Daniel Grundler Florian MW Reuther Marie Cappel Sabrina Bauer Harald Lang Christian Watrin Cliff den Nijs Loes JMF Van Bruggen Anne-Sophie Karssen Gerrit Kiewnick Sebastian Büchler Urs Roth Irma Frey Jürg 《植物病害和植物保护杂志》2015,122(4):189-193
Journal of Plant Diseases and Protection - 相似文献
9.
Hans-Peter Rusterholz Sylvain Ursenbacher Armin Coray Urs Weibel Bruno Baur 《Journal of insect science (Online)》2015,15(1)
The sampling of living insects should be avoided in highly endangered species when the sampling would further increase the risk of population extinction. Nonlethal sampling (wing clips or leg removals) can be an alternative to obtain DNA of individuals for population genetic studies. However, nonlethal sampling may not be possible for all insect species. We examined whether remnants of traffic-killed specimens of the endangered and protected flighless longhorn beetle Iberodorcadion fuliginator (L., 1758) can be used as a resource for population genetic analyses. Using insect fragments of traffic-killed specimens collected over 15 yr, we determined the most efficient DNA extraction method in relation to the state of the specimens (crushed, fragment, or intact), preservation (dried, airtight, or in ethanol), storage duration, and weight of the sample by assessing the quantity and quality of genomic DNA. A modified cetyltrimethyl ammonium bromide method provided the highest recovery rate of genomic DNA and the largest yield and highest quality of DNA. We further used traffic-killed specimens to evaluate two DNA amplification techniques (quantitative polymerase chain reaction [qPCR] and microsatellites). Both qPCR and microsatellites revealed successful DNA amplification in all degraded specimens or beetle fragments examined. However, relative qPCR concentration and peak height of microsatellites were affected by the state of specimen and storage duration but not by specimen weight. Our investigation demonstrates that degraded remnants of traffic-killed beetle specimens can serve as a source of high-quality genomic DNA, which allows to address conservation genetic issues. 相似文献
10.
Alternative poultry production with special reference to free range broilers has increased significantly since the nineties
in many regions of the world. Numerous factors influence the productive performance of this type of broilers: genotype (namely
the use of naked neck animals), feeding and access to an outdoor area. The aim of this paper is to study the influence of
each of these factors on the productive performance of free range broilers under commercial rearing conditions. A total of
3200, day old chicks of both sexes from naked neck and normally feathered genotypes were used in this trial. After a joint
initiation phase, animals were divided into four different treatments with the combination of two concentrates (high vs low
energy content) and management (access to outside park or not). Experiment lasted a total of 12 weeks. Live weight date was
recorded weekly and a samples of animals from the trial were sacrificed at the age of 8, 10 and 12 weeks, when carcass characteristics
were determined. Besides sex, the only factor that seems to affect growth characteristics was genotype as naked neck animals
had poorer growth rates than normally feathered. No effect was detected on carcass yields and percentages of carcass components
for any of the variables. From the data presented in this trial the practises associated with free range production are of
relative inconsequence to the technical animal production parameters and can only be justified by a pressing need to differentiate
these products from standard poultry products in what concerns both welfare issues and meat characteristics. The results also
indicate that genetic material from alternative poultry production in Europe can be a useful option in poultry production
development projects in the tropics. 相似文献