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排序方式: 共有190条查询结果,搜索用时 15 毫秒
1.
Toshihiko Saito 《Fisheries Science》2017,83(3):383-400
Linear discriminant analysis (LDA) based on scale patterns was used to develop a methodology of estimating regional origins of chum salmon. Age-4 fish were sampled in 2004–2006 from 12 river stocks of the Okhotsk and Sea of Japan (SJ) regions from Hokkaido to Honshu. The scale radius at the first annulus of each fish was separated into i intervals and the radius of each interval was divided by the number of scale circuli within the interval to quantify scale patterns. The i variables and five other morphometric measurements were used in a stepwise LDA to classify the following regional groups: Hokkaido and Honshu (I), Okhotsk and SJ (II), Okhotsk, Hokkaido SJ and Honshu SJ (III). Percentages of correctly classified fish (hit rates) improved with increased i but tended to be close to asymptotic values in all cases. Hit rates for each river stock in case (I) ranged from 74.3% to 100% (mean 97.2%), estimated by direct maximum likelihood methods using predictor variable sets from the best models for LDAs. Hit rates were lower in cases (II) and (III). This study demonstrated that scale patterns are useful for classifying the origins of chum salmon, at least between Hokkaido and Honshu. 相似文献
2.
Kazuya KUSHIDA Urs GIGER Toshihiko TSUTSUI Megumi INABA Yoshio KONNO Kureha HAYASHI Kana NOGUCHI Akira YABUKI Keijiro MIZUKAMI Moeko KOHYAMA Yasuyuki ENDO Osamu YAMATO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(6):743-746
Erythrocyte pyruvate kinase (PK) deficiency is an inherited glycolytic erythroenzymopathy
caused by mutations of the PKLR gene. A causative mutation of the feline
PKLR gene was originally identified in Abyssinian and Somali cats in
the U.S.A. In the present study, a TaqMan probe-based real-time PCR genotyping assay was
developed and evaluated for rapid genotyping and large-scale screening for this mutation.
Furthermore, a genotyping survey was carried out in a population of four popular purebred
cats in Japan to determine the current mutant allele frequency. The assay clearly
displayed all genotypes of feline PK deficiency, indicating its suitability for
large-scale survey as well as diagnosis. The survey demonstrated that the mutant allele
frequency in Abyssinian and Somali cats was high enough to warrant measures to control and
prevent the disease. The mutant allele frequency was relatively low in Bengal and American
Shorthair cats; however, the testing should still be carried out to prevent the spread of
the disease. In addition, PK deficiency should always be considered in the differential
diagnosis of anemia in purebred cats in Japan as well as worldwide. 相似文献
3.
Sayuri Okawa Kana Unuma Atsushi Yamada Toshihiko Aki Koichi Uemura 《Journal of toxicologic pathology》2015,28(1):37-41
The involvement of the lung during the septic systemic inflammatory response elicited by administration of lipopolysaccharide (LPS) was investigated. Eight-week-old male Sprague–Dawley rats were injected i.p. with 15 mg/kg LPS. After 24 h, the lungs were excised to evaluate the cellular responses to LPS. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF) analysis revealed that type VI collagen (ColVI) was extremely upregulated during sepsis in the rat lung within the first 24 h of LPS administration. Upregulation of ColVI protein and its mRNA was demonstrated by Western blot analysis, real time PCR, and immunohistochemistry. To the best of our knowledge, this is the first report demonstrating the activation of ColVI in the rat lung at the early stage of systemic inflammation. Activation of ColVI might be involved in sepsis-mediated lung fibrosis at an early stage. 相似文献
4.
Shinpei Banno Hidenari Saito Hiroshi Sakai Toshihiko Urushibara Kentaro Ikeda Takeshi Kabe Isao Kemmochi Makoto Fujimura 《Journal of General Plant Pathology》2011,77(5):282-291
Verticillium longisporum and V. dahliae, causal agents of Verticillium wilt, are spreading through the cabbage fields of Gunma Prefecture. Using the V. longisporum-specific intron within the 18S rDNA and differences between ITS 5.8S rDNA sequences in Japanese isolates of V. longisporum and V. dahliae, we developed three quantitative nested real-time (QNRT) PCR assays. The QNRT-PCR quantification of V. longisporum or V. dahliae in cabbage field soil was consistent with the severity of Verticillium wilt disease in those fields. In field trials of resistant
cultivar YR Ranpo grown for three seasons in soil infested with the pathogen, disease severity and pathogen density in the
soil were significantly reduced in a field moderately contaminated by V. dahliae, but only slightly reduced in a highly contaminated field. These results suggest that continuous cultivation of a resistant
cultivar is an effective way to reduce the pathogen population. QNRT-PCR assays provide a powerful analytical tool to evaluate
the soil population dynamics of V. longisporum and V. dahliae for disease management. 相似文献
5.
6.
A. K. M. Zakir Hossain Md. Ali Asgar M. Alamgir Hossain Toshihiko Tosaki Hiroyuki Koyama Tetsuo Hara 《Soil Science and Plant Nutrition》2005,51(1):43-49
The effects of B and Ca treatments on root growth, nutrient localization and cell wall properties in wheat ( Triticum aestivum L.) plants with and without Al stress were investigated. Seedlings were grown hydroponically in a complete nutrient solution for 7 d and then treated with B (0, 40 μM), Ca (0, 2,500 μM), and Al (0, 100 μM) in a 500 μM CaCl2 solution for 8 d. The cell wall materials (CWM) were extracted with a phenol: acetic acid: water (2:1:1 w/v/v) solution and used for subsequent pectin extraction with trans -1,2-diami-nocyclohexane- N,N,N,N -tetraacetic acid (CDTA) and Na2 CO3 solutions. Boron, Ca, and B + Ca treatments enhanced root growth by 19.5, 15.2, and 27.2%, respectively, compared to the control (pH 4.5). Calcium and B+Ca treatments enhanced root growth with Al stress by 43 and 54%, respectively, while B did not exert any effect. The amounts of CWM and pectin per unit of root fresh weight increased by Al treatment, whereas the Ca and B+Ca treatments slightly reduced the contents of these components. Seventy-four percent of total B, 69% of total Ca, and 85% of total Al were located in the cell wall in the B, Ca, and Al treatments, respectively and 32% of total B, 33% of total Ca, and 33% of total Al were located in the CDTA-soluble and Na2 CO3 -soluble pectin fractions. A more conspicuous localization of B was observed in the presence of Al. Aluminum treatment markedly decreased the Ca content in the cell wall as well as pectin fractions, mainly in the case of the CDTA-soluble pectin fraction. Boron + Ca treatment decreased the Al content in the cell wall and pectin fractions compared to the Ca treatment alone in the presence of Al. It is concluded that the B+Ca treatment enhanced root growth and, B and Ca uptake, and helped to maintain a normal B and Ca metabolism in the cell walls even in the presence of Al. 相似文献
7.
The growth, sex ratio with age, and age at sexual maturation were determined based on sectioned otoliths in 257 specimens
of the blackspot tuskfish Choerodon schoenleinii collected in waters off Ryukyu Island. Opaque rings observed by reflected light in the sectioned otoliths were found to form
once a year from January to July. The three growth parameters of the von Bertalanffy growth equation were L∞ = 68.1 (cm), k = 0.263, and t
0 = −0.023 (year). The age at which the sex ratio reached 50% by sexual transition was about 6.15 years, and the age at which
50% of females were sexually mature was approximately 2 years. The oldest specimen among the samples was 17 years old. 相似文献
8.
Shigeya Maeda Toshihiko Kawachi Koichi Unami Junichiro Takeuchi Tomoki Izumi Syunsuke Chono 《Paddy and Water Environment》2009,7(3):163-175
A fuzzy optimization model is developed to allocate allowable total nitrogen (T-N) loads to distributed nonpoint sources (NPSs)
and point sources (PSs) in a watershed for river water quality management using the linear programing technique. The watershed
is divided into uniform grid cells on which T-N loads issuing from NPSs such as paddy fields, upland crop fields and cities
are controlled. A geographic information system integrated with the digital elevation model facilitates computation of route
lengths of surface and subsurface flows from cells to a river running through the watershed. The T-N loads discharged from
their sources are assumed to decay, subject to distance-related first-order kinetics. As management goals, maximizations of
total allowable NPS loads, total allowable PS loads and total yield of rice are considered from environmental and economic
viewpoints. A prime constraint is an effluent limitation standard for the aggregate amount of loads that arrive at the downstream
end of the river. The fuzzy sets theory helps appropriately describe vague attitudes of decision-makers (i.e., stakeholders
and management authorities) in terms of constraints and conflicting goals. An application of the fuzzy optimization model,
developed as an improvement over our last nonfuzzy model, to a real watershed in Shiga prefecture, Japan, demonstrates that
the fuzzy model embodies our last model, and is capable of creating management alternatives for T-N load allocation in a more
practical and flexible manner. 相似文献
9.
Junichiro Takeuchi Toshihiko Kawachi Koichi Unami Shigeya Maeda Tomoki Izumi 《Paddy and Water Environment》2009,7(1):33-43
A cell-based distributed watershed model is developed which enables us to simulate the hydrological and hydraulic aspects
of the watershed in a refined fashion. With three-zoned cell profiling, the model is composed of three sub-models; tank model
for a surface water zone, soil moisture model for a surface soil zone, and unconfined shallow groundwater flow model for a
subsurface zone. Inclusion of the soil moisture sub-model modified to reroute the infiltration, routed from the tank sub-model,
into the return flow and the groundwater recharge features the model. The groundwater flow sub-model, numerically approximated
by use of the finite volume method and the implicit time-marching scheme, considers a network of on-farm drainage canals as
internal boundaries, which is an essential need for modeling the watershed including farmlands. Cascade-linking of the three
sub-models in a cell and assembling of all the cells over the entire watershed domain provides the global equations system
to be solved. Applicability of the model is demonstrated with its practical application to a real watershed in that paddy
and upland crop fields take great part of the land-use practice. It is then indicated in a quantified manner that rice farming
significantly contribute as a major groundwater recharger in an irrigation period to fostering and conservation of regional
water resources. Along with appropriately profiling a cell, the model is so versatile and tough that it can be applied without
difficulty to a watershed of diverse terrains and land-uses and the computations can stably be carried out. It is thus concluded
that the model presently developed could be a powerful “watershed simulator” to investigate and assess the time-varying hydro-environmental
properties of a watershed while separating and integrating the hydrological and hydraulic components of particular importance. 相似文献
10.
The beneficial effects of horse trekking on autonomic nervous activity in experienced rider with no disability 下载免费PDF全文
Akihiro Matsuura Haruhiro Maruta Tomohiko Iwatake Takashi Kumagai Toshihiko Nakanowatari Koichi Hodate 《Animal Science Journal》2017,88(1):173-179
The aim of this study was to determine whether autonomic nervous activity of a rider with no disability was altered by one practical and applicable horse trekking (HT) exercise. Changes in autonomic nervous activity were analyzed by heart rate variability (HRV). Twenty‐three participants with no disability rode horses along a predetermined HT course at trotting and walking for 60 min. HRV was sampled at 60 min before and immediately, following 60 min, and 120 min after HT. As a control, the same measurements were performed for 22 age‐matched participants during their rest. Only in the HT group, the value of normalized unit in high frequency component (HF nu), an index of parasympathetic nervous activity, was higher at 120 min after treatment than before HT (P < 0.05). The low / high frequency ratio (LF / HF), believed to reflect sympathetic nervous activity, was lower in the HT group than those in the control group at 60 min (P < 0.05) and 120 min after treatment (P < 0.01). These findings suggest that a single HT shifted the autonomic nervous balance of a rider toward parasympathetic dominance. The results obtained by the present study could accelerate the use of horses for human health. 相似文献