Formulation of Indices to Describe Intrinsic Nitrogen Transformation Rates for the Implementation of Best Management Practices in Agricultural Lands

Water, Air, &; Soil Pollution - Mining creates large amounts of processed waste in the form of mine tailings. Sulfide mine tailings are of particular concern due to the biotic and abiotic...     

Assessment of the Intrinsic Vulnerability of Agricultural Land to Water and Nitrogen Losses via Deterministic Approach and Regression Analysis

A set of indices was developed in order to classify the vulnerability of agricultural land to water and nitrogen losses (LOS), setting a basis for the integrated water resources management in agricultural systems. To calibrate the indices using multiple regression analysis, the simulation results of Groundwater Loading Effects of Agricultural Management Systems (GLEAMS) model for combinations of different soil properties, topography, and climatic conditions of a reference field crop were used as “observed values.” GLEAMS quantified (1) the annual losses of the percolated water beneath the root zone, (2) the annual losses of the surface runoff, (3) the annual losses of the nitrogen leaching beneath the root zone, and (4) the annual losses of nitrogen through the surface runoff, which were used to calibrate the following indices LOSW-P, LOSW-R, LOSN-PN, and LOSN-RN, respectively. All the simulations to gain the LOS indices were carried out for the same reference field crop, the same nitrogen fertilization, and the same irrigation practice, in order to obtain the intrinsic vulnerability of agricultural land to water and nitrogen losses. The LOS indices were also combined to derive nitrogen concentrations in the percolated and in the runoff water. Finally, the connection of LOS indices with the groundwater was performed using an additional equation, which determines the minimum transit time of the percolated water to reach the groundwater table.     

Enhancing field scale water productivity for several rice cultivars under limited water supply

Paddy and Water Environment - Rice production is one of the largest consumer of water in agriculture. In general, the irrigation water productivity (WPI) is low in paddy fields. In order to improve...     

Numerical assessment of effective evapotranspiration from maize plots to estimate groundwater recharge in lowlands

To maximize the irrigation efficiency and to protect groundwater from agrochemical pollution, two variables must be known with good accuracy: effective evapotranspiration and infiltration, especially in lowland areas were the run-off is minimal. Three different experimental plots cultivated with maize were equipped with tensiometers and soil moisture probes to monitor every day the water movement in the unsaturated zone. Other relevant parameters of the various soil layers, as hydraulic conductivity and water retention curve, were obtained in laboratory experiments, while boundary conditions, as precipitations, temperature and root growth, were obtained on site. Inverse modeling was performed using HYDRUS-1D to assess the degree of uncertainty on model parameters. Results showed a good model fit of water content and head pressure at various depths, in each site, using Penman-Monteith formula for daily potential evapotranspiration calculation, but poor fit applying the Hargreves and Turk formulas. Best performance of model fit was observed for S-shaped equation employed to simulate the root water-uptake reduction with respect to Feddes equation. The soil parameters uncertainty was limited and remained within analytical errors, thus a robust estimation of cumulative infiltration and evapotranspiration has been derived. This study points out that evapotranspiration is the most important variable in defining groundwater recharge for maize crops in lowlands.     

Reactive Modeling of Denitrification in Soils with Natural and Depleted Organic Matter

Nitrogen fertilizers used in agriculture often cause nitrate leaching towards shallow groundwater, especially in lowland areas where the flat topography minimize the surface run off. In order to introduce good agricultural practices that reduce the amount of nitrate entering the groundwater system, it is important to quantify the kinetic control on nitrate attenuation capacity. With this aim, a series of anaerobic batch experiments, consisting of loamy soils and nitrate-contaminated groundwater, were carried out using acetate and natural dissolved organic matter as electron donors. Acetate was chosen because it is the main intermediate species in many biodegradation pathways of organic compounds, and it is a suitable carbon source for denitrification. Sorption of acetate was also determined, fitting a Langmuir isotherm in both natural and artificially depleted organic matter soils. Experiments were performed in quadruplicate to account for the spatial variability of soil parameters. The geochemical code PHREEQC (version 2) was used to simulate kinetic denitrification using Monod equation, equilibrium Langmuir sorption of acetate, and equilibrium reactions of gas and mineral phases (calcite). The reactive modeling results highlighted a rapid acetate and nitrate mineralization rate, suggesting that the main pathway of nitrate attenuation is through denitrification while calcite acted as a buffer for pH. However, in the absence of acetate, the natural content of organic matter did not allow to complete the denitrification process leading to nitrite accumulation. Reactive modeling is thought to be an efficient and robust tool to quantify the complex biogeochemical reactions which can take place in underground environments.     

Water Resources Development and Water Utilization in the Gavkhuni River Basin,Iran

Analysis of water supplies and demand over the past 50 years in the Gavkhuni River Basin（GRB） indicate that despite large investments in water resources development the basin remains just as vulnerable to drought as it always has been. During the period of analysis two transbasin diversions and a storage reservoir have been constructed which have more or less doubled the annual supply to water to the basin. But with each water resource development extractive capacity for irrigation, urban and industrial use has increased by the same amount, so that all new water is allocated as soon as it is available. The most recent developments, since 1980, have actually increased vulnerability to drought because extractive capacity is greater than average flow into the basin. Whenever demand exceeds supply all water is extracted from the basin and the tail end dries up. During the past 50 years flows into the salt pan at the downstream end of the basin have been negligible for more than half the time. Prospects for the future are bleak because once the current phase of water resources development is completed no further water supplies are likely, but demand continues to rise at a steady rate. Ultimately agriculture will have to concede water to urban, industrial and environmental demands.     

Experimental infection of domestic cats with passaged genotype I Bartonella henselae

The influence of in vitro passage on Bartonella henselae pathogenesis in cats has not been thoroughly evaluated. Our objective was to examine the bacterial kinetics and humoral immune responses in cats experimentally infected with three different in vitro passages of B. henselae F1, a genotype I strain of feline origin. The F1 strain was in vitro passaged 20 and 40 times, and each was inoculated into a group of 5 cats. The kinetics of bacteremia and the feline humoral immune response to bacterial antigens were compared to a previous study involving a group of six cats inoculated with the original F1 strain. Among the three groups of cats, the kinetics of bacteremia profiles and the humoral immune responses to B. henselae lysates were similar. The influence of passage on bacterial membrane proteins was examined. In vitro passage altered the expression of 4/17 (23.5%) bacterial membrane proteins and 6/15 (40%) bacterial membrane antigens. An association between poor seroreactivity to three lysate antigens (15-, 18- and 45kDa), prolonged bacteremia and decreased serum bactericidal activity was noted. Our data show that in vitro passage of B. henselae did not alter the kinetics of bacteremia, including the occurrence of relapsing bacteremia, in experimentally infected cats. This suggests that highly passaged strains may not be suitable for future vaccination studies. Furthermore, in vitro passage results in phenotypic and antigenic changes in the bacterial membrane protein profile, which warrants caution in the interpretation of studies involving passaged B. henselae strains.