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Pedro A. Castrillo Catuxa Varela-Dopico Paz Ondina María Isabel Quiroga Roberto Bermúdez 《Journal of fish diseases》2020,43(1):69-80
Freshwater mussels of the order Unionida encyst into the fish mucosa to metamorphose and complete their life cycle, causing a parasitic disease known as glochidiosis. This parasitic stage represents a bottleneck for the survival of naiads, particularly for critically endangered species as Margaritifera margaritifera; however, little is known about the events occurring during this critical stage. Therefore, this study aimed to histologically characterize the development of M. margaritifera glochidiosis in Atlantic salmon to get insight into the pathogenesis of this interaction. Fish exposed to glochidia were sampled during the first 44 days post-exposure, and organs were observed by stereomicroscopy and light microscopy. Glochidia attached to the gills by pinching the lamellar epithelium, whereupon an acute proliferative branchitis engulfed most of the larvae. However, during the first 14 days, a severe detachment of unviable glochidia occurred, associated with the presence of pleomorphic inflammatory infiltrate and epithelial degeneration. In the cases where larvae remained attached, a chronification of the lesions with none to scarce inflammation was observed. These results provide key information to better understand the complex host–parasite interaction during the early stages of glochidiosis and provide valuable information to optimize artificial rearing of naiads in conservation of threatened freshwater mussel populations. 相似文献
3.
Maria Antonia Machado Barbosa Kacilda Naomi Kuki Pedro Santos Peno Bengala Emilly dos Santos Pereira Angélica Fátima de Barros Sebastián Giraldo Montoya Leonardo Duarte Pimentel 《Journal of Agronomy and Crop Science》2020,206(2):263-276
Environmental conditions influence phenology and physiological processes of plants. It is common for maize and sorghum to be sown at two different periods: the first cropping (spring/summer) and the second cropping (autumn/winter). The phenological cycle of these crops varies greatly according to the planting season, and it is necessary to characterize the growth and development to facilitate the selection of the species best adapted to the environment. The aim of this study was to characterize phenological phases and physiological parameters in sorghum and maize plants as a function of environmental conditions from the first cropping and second cropping periods. Two parallel experiments were conducted with both crops. The phenological characterization was based on growth analyses (plant height, leaf area and photoassimilate partitioning) and gas exchange evaluations (net assimilation rate, stomatal conductance, transpiration and water-use efficiency). It was found that the vegetative stage (VS) for sorghum and maize plants was 7 and 21 days, respectively, longer when cultivated during the second cropping. In the first cropping, the plants were taller than in the second cropping, regardless of the crop. The stomatal conductance of sorghum plants fluctuated in the second cropping during the development period, while maize plants showed decreasing linear behaviour. Water-use efficiency in sorghum plants was higher during the second cropping compared with the first cropping. In maize plants, in the second cropping, the water-use efficiency showed a slight variation in relation to the first cropping. It was concluded that the environmental conditions as degree-days, temperature, photoperiod and pluvial precipitation influence the phenology and physiology of both crops during the first and the second cropping periods, specifically cycle duration, plant height, leaf area, net assimilation rate, stomatal conductance and water-use efficiency, indicating that both crops respond differentially to environmental changes during the growing season. 相似文献
4.
Igor S. T. Vicente Luciana F. Fleuri Pedro L. P. F. Carvalho Matheus G. Guimares Rafael F. Naliato Hinglidj de Carvalho Müller Maria M. P. Sartori Luiz E. Pezzato Margarida M. Barros 《Aquaculture Research》2019,50(1):80-92
This study evaluated the ability of orange peel fragment (OPF) to act as a functional feedstuff, influencing growth, haematological profile, and antioxidant enzyme activity of Nile tilapia subjected heat/dissolved oxygen‐induced stress (HDOIS). A group of 440 male Nile tilapia (31.7 g ± 0.34) was randomly distributed in 40 250‐L aquaria (11 fish/tank) and fed five practical diets with graded levels of OPF at 0%, 0.2%, 0.4%, 0.6%, and 0.8% for 70 days. The diets were formulated to contain 30% crude protein and 18 MJ/kg crude energy. After the feeding period, growth performance was evaluated and six fish per treatment were sampled for haematological profile and antioxidant enzyme activity, before and after HDOIS. Then, fish were subjected to HDOIS (32°C/2.3 mg/L dissolved oxygen) for three days and the same haematological profile and antioxidant enzyme activity were determined. There was no effect of OPF on the haematological profile, either before or after HDOIS. The polynomial regression model was used to express the relationship between antioxidant enzymes activity and OPF supplementation level. The maximum activity of superoxide dismutase, catalase, and glutathione peroxidase was reached at 0.66%, 0.63%, and 0.68% of OPF respectively. Results of the present study suggest that a dietary supplementation level of 0.63%–0.68% of orange peel fragment was appropriate to maintain Nile tilapia haematological profile and improve its antioxidant capacity under HDOIS. 相似文献
5.
Fagner Machado Ribeiro Mrio Lima Pedro Aurlio Tataíra da Costa Diego Machado Pereira Thony Assis Carvalho Tadeu Vilela de Souza Hortência Aparecida Botelho Fabyano Fonseca e Silva Adriano Carvalho Costa 《Aquaculture Research》2019,50(7):2004-2011
The objective of this work was to evaluate the association between morphometric variables and carcass characteristics in Pirapitinga. We used a thousand specimens of Pirapitinga with an average weight of 1,200 g, which were stunned, slaughtered, weighed, measured, and processed for morphometric and processing yield analysis, to obtain weights, carcass and fillet yields. Initially, the linearity of the variables was verified. Pearson's simple and partial correlation tests were performed between all metrics. Track analysis was performed considering the weights and yields of carcass and filet as dependent variables and the others as independent variables. Ridge regression models were used to eliminate the effects of multicollinearity among the independent variables. Observations showed that the simple correlations between body weights and yields were superior to the partial ones in terms of magnitude. The length and circumference of the specimens were the measures most linearly associated with weight, carcass weight and fillet with rib weight. As for carcass yield and fillet yield, linear correlation coefficients were low and not significant when associated with body weights. With the path analysis, we could observe significant positive correlations between the morphometric measurements for weight, carcass weight and fillet with rib weight. The standard length, body circumference and body circumference/body height ratio are the most correlated. The latter are the most important variables in the evaluation of body weights in Pirapitinga fish. They can serve as criteria for indirect selection in searching for fish with better carcass characteristics. As for fillet and carcass yields, the morphometric variables have not shown to be efficient for indirect selection. 相似文献
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7.
Santana Pedro Henrique Lopes Burak Diego Lang Thiengo Cássio Carlette Peçanha Anderson Lopes Neves Mirna Aparecida Mendonça Eduardo de Sá 《Journal of Soils and Sediments》2020,20(11):4103-4110
Journal of Soils and Sediments - After the greatest environmental disaster in the history of Brazil and the deposition of the iron ore tailings in alluvial regions, the process of revegetation for... 相似文献
8.
Eduardo da Silva Manuel Aldegunde Deivisson Ferreira da Silva Carlize Lopes Fabiano Cleber Bertoldi Robilson Antnio Weber 《Aquaculture Research》2020,51(2):577-583
This study aimed to determine the lowest effective concentration (LEC) of 2‐phenoxyethanol (2‐Phe) and essential oils (EOs) of Melaleuca alternifolia and Ocimum gratissimum able to induce anaesthesia in Astyanax bimaculatus. LEC was assumed to be the required concentration to carry out anaesthesia in less than 3 min with recovery in <5 min and sufficient to carry out routine aquaculture procedures. The following concentrations were tested: 2‐Phe (100, 200, 300, 400, 500 and 600 µl/L); M. alternifolia (200, 300, 400, 500 and 600 µl/L); and O. gratissimum (10, 20, 30, 40, 50, 60, 70, 100, 150, 250 and 300 µl/L). Results show that all three agents evaluated have effective anaesthetic properties for use in Astyanax bimaculatus with LEC for 2‐Phe, M. alternifolia and O. gratissimum of 200, 300 and 60 µl/L respectively. 相似文献
9.
Joo Araújo Ana Catarina Matias Pedro Pouso‐Ferreira Florbela Soares 《Aquaculture Research》2020,51(9):3751-3759
The development of new octopus‐based products with a growing economic value ensures the commercial interest of this species, making live octopus export an activity of great interest. The aim of this study was to develop a method for long‐distance transportation of live octopus at high densities. The system was composed by 220‐L tanks with cooling and aeration, where the animals were kept separated from each others. The water temperature was maintained at 10°C, after a decreasing of 1°C/hr. Live octopus transportation was tested for 48 hr at two densities: 50 kg/m3 and 100 kg/m3. During this period, water parameters were monitored. Stress response was evaluated through the analysis of haemolymph, muscle and brain tissues. No mortality was registered after 48 hr for both treatments. In all trials, water quality remained within the normal limits in both densities; there was, however, a significative increase of ammonia levels in the water. Ammonia, dopamine and Hsp70 levels were analysed in the beginning and at the end of the experiment for both densities; however, no significant differences were found among them. In general, this system seems to be a viable solution for live octopus 48‐hr transportation at a density of 100 kg/m3. 相似文献
10.
Kennya Gomes Silva Addam Scheila Anelise Pereira Gabriel Fernandes Alves Jesus Lucas Cardoso Nichola Syracuse Gustavo Ruschel Lopes Nicollas Breda Lehmann Bruno Correa da Silva Lúvia Souza de S Francisco Clio Maia Chaves Maurício Latera Martins Jos Luiz Pedreira Mourio 《Aquaculture Research》2019,50(10):2960-2971
This study evaluated the effects of dietary supplementation of organic acids blend (OAB) alone or in combination with essential oil, Lippia origanoides (OAE) for Nile tilapia fed supplemented diets for 30 days. Fish (1.1 ± 0.04 g) were fed control (Control), or OAB 0.5% or OAB 0.5% + essential oil 0.125% (OAE) respectively. At the end of the experiment, samples were collected for de hemato‐immunological, histological analysis of the intestine and liver, as well as microbiology of the intestine. The pH of the diets supplemented with OAB and OAE reduced 0.92 and 0.19 respectively. The growth and FCR were unaffected by the treatments, but survival was significantly higher in the OAB treatment. Fish fed the OAB diet showed reduced concentration of total heterotrophic bacteria and Pseudomonas sp. in the intestine. Increased glucose in fish fed OAB and high number of circulating monocytes in fish fed OAE diet were observed. The anterior intestine of fish fed OAE diet showed larger number of goblet cells and increased villi height. The diet supplemented with OAB, mainly, improved the intestinal health and survival of tilapia juveniles and can be used in juvenile production. 相似文献