Mouse embryo co‐culture with autologous cumulus cells and fetal development post‐embryo transfer

This study was conducted to examine the potential for implantation and sustainable fetal development of mouse embryos cultured from the pronuclear to blastocyst stage. Pronuclear embryos from ICR mice (Harlan Sprague‐Dawley) were cultured in Sydney IVF sequential media (Cook) to the blastocyst stage in medium only or co‐cultured with autologous cumulus cells. We also experimented with co‐culture in 100 µL drops. Drop co‐culture produced blastocyst formation rates with a mean of 47.0%, which was significantly higher (P < 0.05) compared to embryos cultured in identical culture conditions except without cumulus cells at 27.3%. Blastocysts obtained in vitro in Cook medium only and co‐cultured in Cook medium with cumulus cells were transferred to pseudopregnant females of ICR strain. The day of blastocyst transfer into surrogate females was designated as post‐transfer of blastocyst day 1 (PT 1). The implantation and fetal development was compared to embryo transfer of in vivo derived blastocysts, which served as controls. There were no statistical differences for implantation and fetal development rates for blastocysts cultured in vitro in either Cook medium only or co‐culture in Cook medium with cumulus cells compared to in vivo‐derived blastocysts. The advantage of the co‐culture system is in generating more blastocysts available for transfer.     

Less hazardous alternative herbicides to control weeds in immature oil palm

In 2002, the Malaysian government had banned the use of the hazardous herbicide, paraquat. Most growers perceive that paraquat is the most effective herbicide and provides the fastest mode of action to control weeds. An experiment was conducted at MAB Agriculture-Horticulture, Sepang, Selangor, Malaysia, from February 2004 to February 2005 to evaluate the efficacy and ability of the less hazardous herbicides, glufosinate ammonium and glyphosate, as an alternative to the hazardous herbicide, paraquat, in controlling weeds in immature oil palm (<3 years old). The results showed that paraquat needed high rates, 600 and 800 g ha⁻¹, to control weeds effectively. However, lower rates of glufosinate ammonium (200 g ha⁻¹) and glyphosate (400 g ha⁻¹) gave excellent weed control. The results showed that the efficacy of glufosinate ammonium and glyphosate were much better than paraquat. The results also showed that, with no direct contact with the plants, paraquat, glufosinate ammonium, and glyphosate had no adverse effect on the vegetative and generative growth of oil palm in this study. These results proved that the less hazardous herbicides, glufosinate ammonium and glyphosate, could be used as an alternative to paraquat to control weeds in immature oil palm.     

Virus-induced changes in the response of faba bean to infection by Botrytis

Prior infection of faba bean with the viruses bean yellow mosaic and bean leaf roll increased host susceptibility to subsequent infection by Botrytis fabae and B. cinerea. Cell necrosis beneath inoculum droplets, rate and extent of lesion spread and sporulation of B. fabae were all increased on detached leaves from virus-infected compared with healthy plants. Changes were most marked in young leaves showing conspicuous symptoms of systemic virus infection and in plants virus-infected for at least 2-4 weeks. Localized lesions produced by B. cinerea or a low concentration of B. fabae conidia (10³ spores/ ml) showed increased cell necrosis but were not transformed into aggressive, spreading lesions on virus-infected leaves.     

The effects of sowing depth and flooding on the emergence, survival, and growth of Fimbristylis miliacea (L.) Vahl

Glasshouse experiments were conducted from March to July 2003 to determine the effects of sowing depth, and the time, duration, and depth of flooding on the emergence, survival, and growth of Fimbristylis miliacea (L.) Vahl. The treatments that were evaluated in the first experiment were three seeding depths (0, 0.5, and 1.0 cm), while in the second experiment, three flooding depths (saturated soil with no standing water and soil with water depths of 5 and 10 cm) and three flooding durations (7, 14, and 21 days from sowing) were evaluated. In the third experiment, three flooding depths (saturated soil with no standing water, and soil with water depths of 5 and 10 cm) and four flooding regimes (7, 14, 21 or 28 days after sowing [DAS]) were evaluated. Surface seeding gave the highest emergence rate compared to the 0.5 cm and 1.0 cm soil depths. A significantly higher emergence rate was recorded with the saturated conditions than with the flooded conditions. A flooding duration of ≥ 14 days showed a clear trend of reduced emergence with increasing flooding depth. A significantly higher survival rate, plant height, root length, number of leaves, and dry matter were recorded at soil saturation followed by the 5 cm and 10 cm flooding depths when flooding was simulated at 7 and 14 DAS. When the flooding was delayed to 21 and 28 DAS, the 10 cm flooding depth was required to suppress this weed. The results provide sufficient evidence to confirm that from deeper seed burial (1 cm sowing depth), flooding depths of ≥ 5 cm of durations of 14 and 21 days and at the onset of flooding within 14 DAS were effective in suppressing the emergence and growth of F. miliacea .     

MICROBIAL POPULATIONS AND ENZYME ACTIVITY IN SOIL TREATED WITH HEAVY METALS

Effects of two concentrations (200 and 2000 μg g^-1 soil) of two heavy metals (copper and zinc as sulphates) applied to clay or sandy soil for 12 weeks on the total counts of fungi, bateria and actinomycetes were studied. Activities of three soil enzymes (urease, nitrate reductase and amidase) were also investigated. Application of heavy metals to the clay soil reduced the microbial populations. However, although neither heavy metal showed any significant increasing effect on microorganisms populations in clay soil samples, some stimulatory effects were noted in sandy soil. Activities of urease and nitrate reductase were inhibited by heavy metal application in both soils. Amidase activity was inhibited only with the higher application rate after some experimental periods.     

亚洲土壤和地下水金属污染风险评价---综述近期研究趋势以及现有环境法律法规

Asia is experiencing a more rapid economic growth compared to any other regions. The contamination of soil and groundwater with metals can mainly be attributed to human activities; therefore, risk assessments to characterize the nature and magnitude of risks to humans and ecological receptors from contaminants are important. Risk assessments are often iterative processes, which involve identification and filling data gap. Experimental samplings, geostatistical and multivariate statistical methods as well as multimedia risk assessment modeling are the three major methodologies used in the assessment of metal contamination in soil and groundwater. This review highlights a number of measurements for improving risk calculation methods and expounds scientific approaches that involve the identification of the major source of contamination, exposure pathways and bioavailability of metals. In general, risk assessments of metals in soil and groundwater worldwide are mainly focused on the levels of contamination, identification of exposure pathways, and prediction of the probability of contamination. To date, very limited studies have reported the development of relevant environmental laws and policies in the regulation of soil and groundwater contamination in Asia. The development, variations and limitations in the regulations of soil and groundwater contamination among developed countries may provide helpful guidance for the developing countries in Asia.     

Effects of virus infection of faba bean on subsequent infection by Uromyces viciae-fabae

Prior infection of faba bean with bean yellow mosaic virus (BYMV) and bean leaf roll virus decreased pustule density on leaves subsequently infected by Uromyces viciae-fabae. Changes were most marked on young leaves showing conspicuous symptoms of systemic virus infection and in plants virus-infected for at least 2-4 weeks. Pustule density progressively decreased when inoculations were made with uredospore generations successively produced on and inoculated to BYMV-infected leaves.
Uredospore germination and germ tube length were similar on virus-free and BYMV-infected leaves and on agar seeded with spores produced for one or several generations on BYMV-infected or virus-free plants. Formation of appressoria was slightly reduced on BYMV-infected leaves but changes in the post-penetration development of the rust probably accounted for much of the decreased pustule production encountered.