CORRELATION BETWEEN YIELD,MUST ATTRIBUTES AND NUTRITIONAL STATUS OF THE GREEK RED WINE GRAPE VARIETY ‘AGIORGITIKO’

The appropriate tissue and sampling time for the Greek wine-grape variety ‘Agiorgitiko’ nutritional diagnosis was to be evaluated by relating leaf tissue nutrient contents with the yield and must potassium (K), pH, total soluble solids (TSS), and titratable acidity (TA). At bloom, véraison and harvest, leaves opposite the basal cluster (LOBC) and youngest mature leaves (YML) were harvested, and soil and berry samples were collected from six vineyards in Nemea, Greece. Petiole K concentration of the LOBC was found to be better correlated with the yield, TSS, TA and must K than the blade or whole leaf K content; the contrary was observed in nitrogen (N), phosphorus (P), calcium (Ca), and magnesium (Mg) concentrations. The LOBC reflected better the nutritional status of the variety compared to the YML. Regarding the sampling time, petiole K presented stronger correlations with yield and must K, TSS, TA at harvest whereas N and P at bloom and véraison.     

Pre-Harvest Rind-Spotting in ‘Clementine’ Mandarin

A severe physiological disorder of ‘Clementine’ mandarins in the Assini area of Argolis, Southestern Greece, was investigated for two successive years. Irregular-shaped necrotic spots were observed on the rinds of mandarins, mainly at the fruit stylar-ends, from the color break till harvest time. In order to investigate the possible correlation between the disorder and the inorganic nutrition of the fruit, spotted and symptomless mandarin rinds, in four commercial orchards were chemically analyzed. The relationship between fruit inorganic nutrition and the disorder symptoms were not consistent. Using Electron Scanning Microscopy, invisible cracks were observed at the cuticle and the external strata of the spotted rind whereas oil glands remained intact. Considering the results of chemical analyses, microscopic observations, as well as, the information given by the growers, this physiological disorder should be attributed to sudden changes of climatic conditions and not to nutritional imbalance.     

Effects of Tillage and Fertilization on Biomass, Roots, N-Accumulation and Nodule Bacteria of Vetch (Vicia sativa cv. Alexander)

Field experiments were conducted to determine the effects of tillage (ploughing followed by one rotary hoeing (CT), rotary hoeing (MT) and no-tillage (NT)) and type of fertilization (NP 154/75 kg ha⁻¹, digested farmyard manure 30 t ha⁻¹) on growth (biomass and roots), N-accumulation and nodulation of vetch plants (Vicia sativa cv. Alexander) at different stages. The highest dry matter yields and root weight were determined for the NT and the lowest for the CT systems. The NT system promoted nodulation of vetch plants to a higher degree than the MT and CT systems. At the time just before harvesting, the N-accumulation in the above ground plants, was 54.3 for CT, 76.7 for MT, and 109.0 kg ha⁻¹ for NT, and the corresponding values at roots were 73.3, 112.0 and 173.3 kg ha⁻¹. Growth and nodulation of vetch plants was in general much better on farmyard manure plots than it was with the NP fertilization. The closest correlation coefficients between dry matter of biomass and roots on the one hand, and number of nodules and percentage N in roots on the other, were found with NT. The highest correlation between nodulation and percentage N in roots (r = 0.91***) was observed at the 128-days-old vetch plants. The highest correlation between the number of nodules and percentage N in biomass (r = 0.82**) was found at the beginning of the plants' flowering period. *, **, *** indicate significance at P = 0.05, 0.01 and 0.001 respectively.     

Aristotle as an ichthyologist: Exploring Aegean fish diversity 2,400 years ago

In an attempt to trace the origins of the science of ichthyology, we studied Aristotle's zoological works (21 books in total), aiming to give an exhaustive catalogue of his fishes, suitable for use by modern marine biologists, and to evaluate the great philosopher's knowledge of fish biology. Our investigation yielded 135 ichthyonyms used to describe 109 individual fish taxa (13 fishes had multiple names) and 12 terms that corresponded either to fish developmental stages or behavioural patterns. Because of the variable amount of information given in the texts, 54 fishes were identified to species or genus level, 24 fishes to higher categories (family, order), 27 fishes could only be classified as Actinopterygii or Elasmobranchii, while four could not be classified at all. Among the most easily recognizable fishes were Lophius spp., Conger conger, Muraena helena, Anguilla anguilla and Mugil cephalus. The latter two and the tunas (Τhunnus spp.) were the most frequently recorded fishes. Aristotle's inquiry was not limited or focused on fish that were of human interest, but he also reported or described species that fascinated him by their peculiar life history. He was interested in a wide array of aspects of fish biology ranging from body structure and function, reproduction and development to fish feeding habits, migrations and diseases. As evidenced by his work, Aristotle should be considered the first ichthyologist, preoccupied with the zoological, scientific study of fish, and a review of his writings can prove useful for current ichthyological research.     

Performance of diagnostic tests for the detection and identification of <Emphasis Type="Italic">Pseudomonas syringae</Emphasis> pv. <Emphasis Type="Italic">actinidiae</Emphasis> (Psa) from woody samples

The aim of this study was to characterise the performance of new molecular methods for the detection and identification of Pseudomonas syringae pv. actinidiae (Psa) and to provide validation data in comparison to the assays mentioned in official diagnostic protocols and being currently used. Eleven molecular tests for the Psa detection were compared in an inter-laboratory comparison where each laboratory had to analyse the same panel of samples consisting of thirteen Psa-spiked kiwifruit wood extracts. Laboratories had to perform also isolation from the wood extracts. Data from this interlaboratory test performance study (TPS) was statistically analysed to assess the performance of each method. In order to provide complete validation data, both for detection and identification, this TPS was supplemented by a further study of identification from pure culture of phylogenetically closely related Pseudomonas spp., Psa, and bacterial strains associated with kiwifruit. The results of both these studies showed that simplex-PCRs gave good results, whereas duplex-PCR and real-time PCR were the most reliable tools for detection and identification of Psa. Nested and multiplex-PCR gave false-positive results. The use of the most reliable detection test is suggested for routine analyses, but when Psa-free status needs to be accurately assessed, it is recommended that at least two detection tests are used. This work provides a wide comparison of the available diagnostic methods, giving new information for a possible revision of the official diagnostic protocols (e.g. European and Mediterranean Plant Protection Organization (EPPO) protocol PM7/120 for the detection of Psa).     

Geographic distribution of chestnut feeding insects in Greece

Greece has been traditionally considered as a chestnut-producing country; yet, chestnut production has experienced a dramatic decline in the last decades, mainly due to chestnut blight Cryphonectria parasitica and chestnut feeding pests. Though chestnut blight is partially under control, little attention has been granted on chestnut feeding pests in Greece. Two species are described as major pests, i.e., Cydia splendana and Curculio elephas, without any information regarding their distribution. This study aimed to identify species diversity that feed on chestnuts trees using genetic markers and further to obtain insight into their distribution. Besides C. splendana and C. elephas, three additional species were determined namely Cydia fagiglandana, Curculio glandium as well as a Curculio sp. that has never been genetically determined before. The distribution of these species was inhomogeneous in Greece. These results demonstrate clearly the need for a carefully planned pest management approach adapted to the local mixture of chestnut feeding pests of each area rather than one general approach.