Neuroprotective Metabolites from Vietnamese Marine Derived Fungi of Aspergillus and Penicillium Genera

Low molecular weight secondary metabolites of marine fungi Aspergillus flocculosus, Aspergillus terreus and Penicillium sp. from Van Phong and Nha Trang Bays (Vietnam) were studied and a number of polyketides, bis-indole quinones and terpenoids were isolated. The structures of the isolated compounds were determined by 1D and 2D NMR and HR-ESI-MS techniques. Stereochemistry of some compounds was established based on ECD data. A chemical structure of asterriquinone F (6) was thoroughly described for the first time. Anthraquinone (13) was firstly obtained from a natural source. Neuroprotective influences of the isolated compounds against 6-OHDA, paraquat and rotenone toxicity were investigated. 4-Hydroxyscytalone (1), 4-hydroxy-6-dehydroxyscytalone (2) and demethylcitreoviranol (3) have shown significant increasing of paraquat- and rotenone-treated Neuro-2a cell viability and anti-ROS activity.     

Genes for resistance to powdery mildew in European barley cultivars registered in the Czech Republic from 2011 to 2015

Barley (Hordeum vulgare) is cultivated on 49.1 million hectares worldwide with 50.2% of the area located in Europe. Powdery mildew, caused by Blumeria graminis f. sp. hordei (Bgh), occurs wherever barley is grown. Cultivar resistance plays an important role in global barley production, especially in parts of Europe where high concentrations of both spring and winter types are grown. The aim of this report was to postulate specific resistance genes in barleys from nine European countries registered in the Czech Republic from 2011 to 2015. Thirty‐five spring cultivars and 27 winter barleys were tested with 56 diverse Bgh isolates. Twenty‐five known resistance genes were postulated, and unknown genes were detected in Sandra, Saturn and Zeppelin. Unidentified specific resistance genes were also present in winter hybrids Hobbit and Wootan. Spring cultivars Arthur and Francin consisted of three and two genotypes, respectively. Resistance gene mlo was present in 26 spring cultivars, and the proportion of cultivars with this gene increased from 62.9% in 2006–2010 to 75.7% in 2011–2015. The gene Mlp1 was identified for the first time in German winter cultivar Saturn. Five spring cultivars registered in Slovakia were included in the tests. All the cultivars that were tested contained one or more specific resistance genes to powdery mildew. Adaptability of the pathogen and possibilities for breeding winter barleys are discussed.     

A bioactive biflavonoid from Campnosperma panamense

Lanaroflavone (1), a biflavonoid isolated from the methanol extract of the aerial part of Campnosperma panamense by bioguided fractionation, has been assessed for in vitro antiprotozoal activity. Lanaroflavone showed both antimalarial and leishmanicidal activities, but was inactive against Chagas disease vector, Trypanosoma cruzi.     

Bacterial community structure in lake sediments of microcosms contaminated with nonylphenol

The impact of nonylphenol, an estrogenic degradation product of alkylphenol polyethoxylates, on the bacterial community structure in contaminated sediments of aquatic microcosms was investigated over a period of 20 weeks using a 16S rDNA-based molecular phylogenetic approach. All microcosms showeda strong seasonal fluctuation of the dominant as well as the active bacterial microflora independent of their degree of contamination with nonylphenol. These changes were correlated with the dynamic of the total organic carbon content (TOC), ranging from 4–39 g/kg sediment dry weight and the redox potential in the sediment. Even at the highest observed nonylphenol concentration (3.4 mg/kg sediment dry weight) the bacterial community structure was mostly unchanged.     

Einfluß der Mineralstoffversorgung auf die Prädisposition von Phaseolus vulgaris gegen Colletotrichum lindemuthianum (Sacc. et Magn.) Br. et Cav. II. Organische und anorganische Inhaltsstoffe in Beziehung zur Prädisposition bei variierter N−, K− und Ca−Versorgung in Nährlösungskultur

Influence of mineral nutrition on the predisposition of Phaseolus vulgaris against Colletotrichum lindemuthianum (Sacc. et Magn.) Br. et Cav. II. Organic and anorganic components in relation to predisposition with varying N-, K- and Ca supply

• 1 The predisposition of Phaseolus vulgaris vs. Colletotrichum lindemuthianum was increased with higher N- and K-concentrations in the nutrient solution.
• 2 No correlation between these changes of susceptibility and organic plant constituents, the content of which was also influenced by the nutrition (total carbohydrates, chlorophyll, total N, precipitable N, total protein, individual amino acids).
• 3 Analysis of inorganic plant constituents (K, Ca, Mg, Fe, at increased N- and K-nutrition showed a negative correlation between the predisposition and the content of Ca and, to a lesser extent of Mg.
• 4 The influence of N-nutrition on the susceptibility was only related to the ammonium-ion. The nitrate concentration in the nutrient solution had no effect.
• 5 The predisposition was also influenced by Ca-nutrition: low Ca-concentration in the nutrient solution, low Ca-content and high susceptibility, on the other hand high Ca-concentration, high Ca-content and low susceptibility. At a certain level of Ca no further reduction of the disease level could be achieved (35 and 25mg Cdg dry matter for primary leaves and the third leaf, respectively).
• 6 The relation between N- and K-nutrition and Ca-content is presumably caused by the ion uptake antagonism between K′, NH4′ and Ca2+.

     

Über die Veränderungen der Sorptionseigenschaften von Wurzeln zweier Pflanzenarten während der Verrottung

Die Steigerung der naturalen Erträge unserer Kulturpflanzen ist gegenwärtig kein wissenschaftlicher Arbeitsgegenstand der produktionsorientierten landwirtschaftlichen Forschung. Es bestehen häufig Bedenken, daß mit einer zunehmenden Ertragshöhe unerwünschte Wirkungen auf die Umwelt verbunden sind.

An ausgewählten Beispielen wird dargelegt, daß es zum einen nicht möglich ist, aus der Ertragshöhe oder den Ertragssteigerungen pauschal auf negative Umweltwirkungen der ackerbaulichen Produktion zu schließen. Zudem wird diskutiert, welche Bedeutung die Steigerung der Erträge von Kulturpflanzen für die Sicherung der technologischen, aber auch der ökologischen Ansprüche hat.

Ein Rückgang der Forschungs‐ und Publikationsleistungen zu Anbauverfahren bzw. dem Ertragsmanagement wird für die letzten Jahre dargelegt und dessen Bedeutung für die wissenschaftlichen Arbeiten der landwirtschaftlichen Produktion erörtert.     

The source of autumn and spring downstream migrating juvenile Atlantic salmon in a small lowland river

Over 3 years, 32,444 age‐0 group Atlantic salmon (Salmo salar) parr tagged with passive integrated transponder tags throughout the River Frome catchment were assigned to one of three groups, nonmigrants, autumn migrants and spring smolts, depending on the detection and the timing of detection at downstream tag readers (situated 8.6 km above the tidal limit). We examined the effect of density at the time of tagging (n·m^?2), distance upstream from the tidal limit (km), fish length (mm), Fulton condition index, habitat type (divided into two types, main river and carrier), days after 1 September that each fish was tagged and year (replication) on the proportions of fish in each of the migration groups. Distance upstream from tidal limit was strongly negatively related to the proportion of autumn migrants and positively related to the proportion of spring smolts. Nonmigrants had a lower average body size than migrants, although there were no differences in the sizes of autumn migrants and spring smolts in September prior to migration. Fish density had no effect on migration strategy. A lower proportion of fish migrated as autumn migrants from the smaller carrier habitats than the main river channel. There is some evidence that those parr destined to become autumn migrants underwent a lower mortality rate during September before tagging than those destined to become spring migrants indicating possible physiological or behavioural differences between these two groups of fish at that time. More research into the factors responsible for initiating the autumn migration is required.