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[目的]筛选蚕沙纤维素降解菌株,为蚕沙无害化快速腐熟处理提供优质菌种.[方法]以蚕沙为材料,利用CMC-Na培养基分离蚕沙纤维素降解菌,通过形态学、生化特征和16S rDNA序列测定方法等对分离获得的纤维素降解菌进行分类学鉴定,并研究碳源、氮源、pH、培养时间和培养温度对分离菌株产酶活力的影响.[结果]从蚕沙中筛选出1株高温型细菌,命名为HB-2菌株.根据菌体大小、鞭毛着生位置及数量、16S rDNA核酸序列分析,并结合生理生化特性,HB-2菌株鉴定为短小芽孢杆菌(Bacillus pumilus).HB-2菌株最佳产酶条件为复合碳源(蚕沙+微晶纤维素+米糠+麸皮)、复合氮源(蛋白胨+酵母膏),产纤维素酶最适反应pH 6.5,最适温度35℃.[结论]HB-2菌株CMCase活性较敏感且产酶量大,具有制成菌剂应用于蚕沙无害化快速腐熟的潜力. 相似文献
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旨在探讨一种促进鸡粪快速发酵腐熟、生产优质有机肥料的技术体系。以鸡粪为主要原料,添加定量滤泥以及按不同比例添加金针菇菌糠堆制生产有机肥,测定不同配比堆料发酵过程的温度、pH值、含水量、有机质含量、氮磷钾含量和种子发芽率等有机肥质量指标。结果表明,添加菌糠能有效缩短鸡粪堆肥发酵腐熟时间,不同配方处理生产有机肥各项质量指标均符合有机肥新标准NY/T 525-2021的要求。其中以30%菌糠、60%鸡粪和10%生物干化污泥配合处理的发酵腐熟时间最短,该处理生产的有机肥的总养分含量达10.33%,有机质含量48.5%,含水量32.5%,种子发芽指数106.2%,pH 7.38。菌糠与鸡粪按科学配比堆肥是大宗农业废弃物料无害化处理和资源化利用的重要途径。 相似文献
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百香果疮痂病病原菌鉴定 总被引:1,自引:0,他引:1
To identify the pathogen causing scab disease on the fruit of Passiflora Linn. in the passion fruit yard of Cenxi and Nanning cities, Guangxi province. The scab tissues collected from diseased fruits were isolated,purified,and made pathogenicity test,morphological observation, biological and molecular identification. The representive isolate B31, obtained from the diseased fruit, was selected for morphological observation and pathogen identification, and was verified as the causal agent of the scab disease through Koch’s postulates. The result of morphological observation using light microscope showed that the isolate B31 was very similar to Alternaria in terms of hyphae and spore morphology. Phylogenetic analysis results using multiple gene loci showed that identities of sequences of ITS, Alt al, TEF 1 and endoPG genes of isolate B31 were 100% identical to those of Alternaria alternata. This study clarifies that the pathogen causing scab disease of passion fruit in Guangxi is A. alternata, and this is the first report of A. alternate causing scab on passion fruit in China. 相似文献