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Marker‐assisted backcrossing to develop foliar disease‐resistant genotypes in TMV 2 variety of peanut (Arachis hypogaea L.) 下载免费PDF全文
Rohini M. Kolekar Mallenahally Sukruth Kenta Shirasawa Hajisaheb L. Nadaf Babu N. Motagi Sattigarahalli Lingaraju Prakashgouda V. Patil Ramesh S. Bhat 《Plant Breeding》2017,136(6):948-953
TMV 2 is a very popular peanut variety among the Indian farmers, but it is highly susceptible to fungal foliar diseases like late leaf spot (LLS) and rust. Marker‐assisted backcrossing (MABC) in TMV 2 using foliar disease‐resistant donor, GPBD 4 and the disease resistance‐linked markers (GM2009, GM2079, GM2301, GM1839 and IPAHM103) resulted in a large number of backcross populations and also straight cross populations. Foreground selection followed by field evaluation under disease epiphytotic conditions could identify a few superior genotypes. Two homozygous backcross lines TMG‐29 and TMG‐46 showed enhanced resistance to LLS and rust diseases (score of 3.00 for both) along with 71.0% and 62.7% increase in the pod yield per plot, respectively, over the check, TMV 2. These foliar disease‐resistant and productive lines can be released as commercial varieties or can be used as genetic resources in the peanut improvement. 相似文献
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Sharanabasappa B. Yeri Kenta Shirasawa Manish K. Pandey M. V. C. Gowda Vanhi Sujay Manda Shriswathi Hajisaheb L. Nadaf Babu N. Motagi S. Lingaraju A. R. S. Bhat Rajeev K. Varshney P. U. Krishnaraj Ramesh S. Bhat 《Plant Breeding》2014,133(1):80-85
Heterogeneous inbred families segregating for rust resistance were identified from the two crosses involving susceptible (TAG 24 and TG 26) and resistant (GPBD 4) varieties of peanut. Rust‐resistant (less than score 5) and rust‐susceptible (more than score 5) plants were identified in each HIF and evaluated under rust epiphytotic conditions. The set of plants belonging to the same HIF, but differing significantly in rust resistance, not in other morphological and productivity traits, was regarded as near‐isogenic lines (NILs). Largely, rust‐resistant NILs had GPBD 4‐type allele, and susceptible NILs carried either TAG 24 or TG 26‐type allele at the three SSR loci (IPAHM103, GM1536 and GM2301) linked to a major genomic region governing rust resistance. Comparison of the remaining genomic regions between the NILs originating from each of the HIFs using transposon markers indicated a considerably high similarity of 86.4% and 83.1% in TAG 24 × GPPBD 4 and TG 26 × GPBD 4, respectively. These NILs are useful for fine mapping and expression analysis of rust resistance. 相似文献
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