雷帕霉素预处理延缓牡丹‘洛阳红’切花衰老的生理效应

为研究雷帕霉素靶标激酶TOR在牡丹开花衰老过程中的生理作用，以牡丹（Paeonia suffruticosa Andr.）‘洛阳红’切花为试材，用0.01 μmol • L-1雷帕霉素预处理2 h后瓶插，并测定瓶插寿命、能量物质含量、糖分含量、乙烯释放速率、呼吸速率、MDA含量以及PsTOR、PsSnRK1和PsHXK1表达量变化。结果表明：雷帕霉素预处理可延长该牡丹切花的瓶插最佳观赏期和增大花朵的最大花径；另外，雷帕霉素预处理还可提高花瓣可溶性糖含量和能荷积累，降低呼吸耗能和MDA含量，并下调瓶插初期PsSnRK1和PsHXK1的表达水平，上调瓶插后期PsTOR和PsSnRK1的表达水平。说明雷帕霉素可通过TOR途径调控能量感知，延缓牡丹切花的开放和衰老进程，进而提高瓶插品质。     

INTER‐ AND INTRAFRACTION MOTION FOR STEREOTACTIC RADIOSURGERY IN DOGS AND CATS USING A MODIFIED BRAINLAB FRAMELESS STEREOTACTIC MASK SYSTEM

Precise and accurate patient positioning is necessary when doing stereotactic radiosurgery (SRS) to ensure adequate dosing to the tumor and sparing of normal tissues. This prospective cross‐sectional study aimed to assess feasibility of a commercially available modified frameless SRS positioning system for use in veterinary radiotherapy patients with brain tumors. Fifty‐one dogs and 12 cats were enrolled. Baseline and verification CT images were acquired. The verification CT images from 32 dogs and five cats had sufficient images for fusion to baseline CT images. A rigid box‐based fusion was performed to determine interfraction motion. Forty‐eight dogs and 11 cats were assessed for intrafraction motion by cine CT. Seventy percent of dogs and 60% of cats had interfraction 3D vector translational shifts >1 mm, with mean values of 1.9 mm in dogs, and 1.8 mm in cats. In dogs muscle wasting was weakly correlated with translational shifts. The maximum angular interfraction motion observed was 6.3° (roll), 3.5° (pitch), and 3.3° (yaw). There was no correlation between angular interfraction motion and weight, brachycephaly, or muscle wasting. Fifty‐seven percent of dogs and 50% of cats had respiration‐related intrafraction motion. Of these, 4.5% of dogs and 10% of cats had intrafraction motion >1 mm. This study demonstrates the modified Brainlab system is feasible for SRS in dogs and cats. The smaller cranial size and difference in anatomy increases setup uncertainty in some animals beyond limits usually accepted in SRS. Image‐guided positioning is recommended to achieve clinically acceptable setup accuracy (<1 mm) for SRS.     

落叶松落叶病预测预报技术的研究

我们通过对气象因子与落叶松落叶病发病关系的研究,和对入选的主要气象因子在各月份中作用的分析,将六七月份降水量、平均相对湿度和四五月份平均气温确定为病情测报因子。其当年值预测式为y=b_0+b_1x_1+b_2x_2……+b_(12)x_(12)(x_1、x_2……x_(12)为前1—12年测报因子观测值;b_0、b_1……b_(12)为相应回归系数);由23年全省历史资料建立的病情指数预测式为y=68.31-1.68x_1-0.5638x_2+0.36x_3(x_1、x_2、x_3分别为气温、湿度、降水预测值);全省发病面积预测式为y=(-0.13325+0.00533x)Sy(式中,x为病情指数预测值;Sy为全省幼龄落叶松人工林面积)。根据各地区发病面积比例系数和各地区轻、中、重发病面积的百分比,可预测各地区发病面积和轻、中、重发病面积。应防治面积为重、中级发病面积与20％轻病级面积之和。     

Leslie矩阵模型在油松毛虫种群动态分析中的应用研究

油松毛虫(Dendrolimus tabulaeformis)是我国华北地区油松林的重要害虫之一。其分布面积广、发生频繁、危害严重。有人曾对油松毛虫种群的生物学特性、防治以及种群动态的研究作过有关的报道,本文则通过对油松毛虫的数量变动进行跟踪调查,并用Leslie矩阵模型对其种群动态进行了模拟,这对害虫的测报具有重要的实践意义。一、材料与方法 (一)标准地概况 1984—1986年与1988—1989年我们分别在密云县五座楼场和石墙沟村选择了5块油松纯林作为标准地,概况列在表1中:     

竹地板薄木贴面工艺试验

为了丰富竹地板的外观效果,扩大竹地板的种类,采用正交试验方法,探讨了竹地板表面进行0.6 mm榉木和2.0 mm枫木薄木贴面工艺。结果表明:利用榉木和枫木薄木贴面竹地板切实可行,其较佳工艺参数分别为:榉木热压压力0.8 MPa,热压温度100℃,热压时间240 s,涂胶量为120 g/m2;枫木热压压力1.2 MPa,热压温度120℃,热压时间360 s,涂胶量140 g/m2。     

Tree regeneration and plant species diversity responses to vegetation control following a major windthrow in mixed broadleaved stands

By increasing resource availability, canopy opening enhances tree recruitment as well as the development of neighbouring vegetation. The proliferation of early successional and highly competitive vegetation may have dramatic consequences on seedling establishment. However, differences in competitive abilities have been shown among the plant growth forms commonly encountered in forests. We may thus expect that vegetation management leading to control of different plant growth forms would have different consequences on tree seedling growth and development. To test this hypothesis, we analysed the effects of an intensity gradient of four vegetation control treatments (untreated, coppice control, coppice and non-tree plant control, and coppice, non-tree and pioneer (tree) plant control) on plant species richness and natural tree regeneration in three post-storm sites. Higher plant species richness and a better natural tree regeneration were observed in the more intensive treatments that significantly improved the balance of the relative abundance of tree species. Suppressing the more competitive vegetation, mainly Rubus fruticosus and graminoids, led to the recruitment and growth of tree species sensitive to competition and good tree species diversity. Practical recommendations in terms of vegetation control relative to tree regeneration and plant species richness are given.     

利用百合鳞片组织诱导育苗的试验研究

球根花卉之一百合,以其球根鳞片组织为外植体进行诱导培养,经过多组合培养基对比试验,筛选出各培养阶段最适宜的培养基:诱导分化,MS 6BA3.0mg/L NAA0.2 mg/L;继代增殖,MS 6BA3.0 mg/L IAA0.1mg/L;生根培养,1/2MS IAA2.0 mg/L.     

Relative contribution of phytates, fibers, and tannins to low iron and zinc in vitro solubility in pearl millet (Pennisetum glaucum) flour and grain fractions

In vitro digestions were performed on pearl millet flours with decreased phytate contents and on two dephytinized or nondephytinized pearl millet grain fractions, a decorticated fraction, and a bran fraction with low and high fiber and tannin contents, respectively. Insoluble residues of these digestions were then incubated with buffer or enzymatic solutions (xylanases and/or phytases), and the quantities of indigestible iron and zinc released by these different treatments were determined. In decorticated pearl millet grain, iron was chelated by phytates and by insoluble fibers, whereas zinc was almost exclusively chelated by phytates. In the bran of pearl millet grain, a high proportion of iron was chelated by iron-binding phenolic compounds, while the rest of iron as well as the majority of zinc were chelated in complexes between phytates and fibers. The low effect of phytase action on iron and zinc solubility of bran of pearl millet grain shows that, in the case of high fiber and tannin contents, the chelating effect of these compounds was higher than that of phytates.     

Usefulness of amino acid composition to discriminate between honeydew and floral honeys. Application to honeys from a small geographic area

With the aim of finding methods that could constitute a solid alternative to melissopalynological and physicochemical analyses to determine the botanical origin (floral or honeydew) of honeys, the free amino acid content of 46 honey samples has been determined. The honeys were collected in a small geographic area of approximately 2000 km(2) in central Spain. Twenty-seven honey samples were classified as floral and 19 as honeydew according to their palynological and physicochemical analyses. The resulting data have been subjected to different multivariant analysis techniques. One hundred percent of honey samples have been correctly classified into either the floral or the honeydew groups, according to their content in glutamic acid and tryptophan. It is concluded that free amino acids are good indicators of the botanical origin of honeys, saving time compared with more tedious analyses.     

基于故障树的电控汽油发动机无法启动故障诊断分析

汽车无法启动是常见故障,在短时间里寻找故障发生的原因往往相当困难。故障树分析法作为分析系统可靠性和安全性的一种重要方法,为复杂系统的故障查找提供了一种有效的途径。以奇瑞A3无法启动为例,建立了发动机无法启动的故障树模型,设计出了发动机无法启动故障诊断流程图,结合诊断的实际经验与相应的辅助检查,能够对汽油发动机无法启动故障发生的部位作出快速准确的判断。