雷帕霉素预处理延缓牡丹‘洛阳红’切花衰老的生理效应

为研究雷帕霉素靶标激酶TOR在牡丹开花衰老过程中的生理作用，以牡丹（Paeonia suffruticosa Andr.）‘洛阳红’切花为试材，用0.01 μmol ? L-1雷帕霉素预处理2 h后瓶插，并测定瓶插寿命、能量物质含量、糖分含量、乙烯释放速率、呼吸速率、MDA含量以及PsTOR、PsSnRK1和PsHXK1表达量变化。结果表明：雷帕霉素预处理可延长该牡丹切花的瓶插最佳观赏期和增大花朵的最大花径；另外，雷帕霉素预处理还可提高花瓣可溶性糖含量和能荷积累，降低呼吸耗能和MDA含量，并下调瓶插初期PsSnRK1和PsHXK1的表达水平，上调瓶插后期PsTOR和PsSnRK1的表达水平。说明雷帕霉素可通过TOR途径调控能量感知，延缓牡丹切花的开放和衰老进程，进而提高瓶插品质。

Physiological Effects of Rapamycin Pretreatment on Delaying the Senescence of Cut Flower in Tree Peony‘Luoyanghong’

In order to reveal effects of TOR on opening and senescence of tree peony cut flowers and its physiological mechanism，we studied vase life，energy substances content，sugar content，ethylene production rate，respiratory rate，MDA content and PsTOR，PsSnRK1 and PsHXK1 relative expression level using cut flowers of potted tree peony‘Luoyanghong’under the pretreatment of 0.01 μmol ? L-1 rapamycin for 2 h. The results indicated that rapamycin increased the best viewing period and the maximum flower diameter，increased soluble sugar content and energy charge in the petals，decreased the respiration consumption and MDA content，down-regulated expression of PsTOR，PsSnRK1 and PsHXK1 genes in the early period，and up-regulated expression of PsTOR and PsSnRK1 genes in the later periods. The data suggest that rapamycin can regulate energy-aware through TOR pathway to delay the florescence and senescence of cut tree peony flowers，thus improving the quality of cut tree peony flowers during vase periods.