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Tomoaki Takahashi Kazukiyo Yamamoto Yoshimichi Senda Masashi Tsuzuku 《Journal of Forest Research》2005,10(4):305-312
This study investigated which predictor variables with respect to crown properties, derived from small-footprint airborne light detection and ranging (LiDAR) data, together with LiDAR-derived tree height, could be useful in regression models to predict individual stem volumes. Comparisons were also made of the sum of predicted stem volumes for LiDAR-detected trees using the best regression model with field-measured total stem volumes for all trees within stands. The study area was a 48-year-old sugi (Cryptomeria japonica D. Don) plantation in mountainous forest. The topographies of the three stands with different stand characteristics analyzed in this study were steep slope (mean slope ± SD; 37.6° ± 5.8°), gentle slope (15.6° ± 3.7°), and gentle yet rough terrain (16.8° ± 7.8°). In the regression analysis, field-measured stem volumes were regressed against each of the six LiDAR-derived predictor variables with respect to crown properties, such as crown area, volume, and form, together with LiDAR-derived tree height. The model with sunny crown mantle volume (SCV) had the smallest standard error of the estimate obtained from the regression model in each stand. The standard errors (m3) were 0.144, 0.171, and 0.181, corresponding to 23.9%, 21.0%, and 20.6% of the average field-measured stem volume for detected trees in each of these stands, respectively. Furthermore, the sum of the individual stem volumes, predicted by regression models with SCV for the detected trees, occupied 83%–91% of field-measured total stem volumes within each stand, although 69%–86% of the total number of trees were correctly detected by a segmentation procedure using LiDAR data. 相似文献
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Ogawa T Gamoh K Aoki H Kobayashi R Etoh M Senda M Hirayama N Nishimura M Shiraishi R Servat A Cliquet F 《Zoonoses and public health》2008,55(6):323-327
A virus neutralizing test using an indirect immunoperoxidase technique (VNT-IIP) for rabies has been developed for the titration of dog and cat serum samples in Japan. The VNT-IIP has the advantage that results obtained can be viewed by the naked eye. The purpose of this study was to validate the VNT-IIP and compare it with one of the international standard methods, the fluorescent antibody virus neutralization test (FAVNT). The VNT-IIP showed satisfactory repeatability, high analytical specificity and good accuracy. Regarding the comparison between the VNT-IIP and the FAVNT, the VNT-IIP showed good agreement (91.9%), high sensitivity (92.8%) as well as specificity (87.0%) and good correlation (r = 0.92). As described above, the validation of the VNT-IIP was satisfactory and the performances of the test proved to be equivalent to those of an international standard method. 相似文献
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Daisuke Fujita Analiza G. Tagle Leodegario A. Ebron Yoshimichi Fukuta Nobuya Kobayashi 《Breeding Science》2012,62(1):18-26
Total spikelet number per panicle (TSN) is one of the most important traits associated with rice yield potential. This trait was assessed in a set of 334 chromosomal segment introgression lines (ILs: BC3-derived lines), developed from new plant type (NPT) varieties as donor parents and having the genetic background of an indica-type rice variety IR64. Among the 334 ILs, five lines which had different donor parents and showed significantly higher TSN than IR64 were used for genetic analysis. Quantitative trait locus (QTL) analysis was conducted using F2 populations derived from crosses between IR64 and these ILs. As a result, a QTL for high TSN (one from each NPT donor variety) was detected on common region of the long arm of chromosome 4. The effect of the QTL was confirmed by an increase in TSN of five near-isogenic lines (NILs) developed in the present study. The variation in TSN was found among these NILs, attributing to the panicle architecture in the numbers of primary, secondary and tertiary branches. The NILs for TSN and the SSR markers linked to the TSN QTLs are expected to be useful materials for research and breeding to enhance the yield potential of rice varieties. 相似文献
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Establishment of a potency test by ELISA for a rabies vaccine for animal use in Japan 总被引:2,自引:0,他引:2
Gamoh K Shimazaki Y Senda M Makie H Itoh O Muramatsu M Hirayama N Hatakeyama H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(6):685-688
The ELISA we developed was able to determine the antigen content and was suitable for a potency test, and we described a relative potency assay method which determines the potency of test vaccines by comparing the ELISA value of a test vaccine to that of a reference vaccine. In the present study, we standardized the reference vaccine used for determining the potencies of test vaccines, and established a potency test by ELISA. We evaluated the proposed reference vaccine by the neutralizing antibody responses in dogs after vaccination, by the challenge protection test in guinea pigs (GP potency test), which is the earlier official potency test used in Japan, and by the NIH potency test, which is widely used throughout the world. The results showed that a 4-fold dilution of the proposed reference vaccine induced sufficient immunity in dogs. A 3-fold dilution of the proposed reference vaccine passed the GP potency test. The international units (IU) calibrated by the NIH potency test were 3.7 IU/dose. From the results and the WHO recommendation that veterinary rabies vaccines should have a potency of at least 1.0 IU/dose, we determined to dilute the proposed reference vaccine by 3 fold and regarded it as the reference vaccine. Finally, we confirmed that there is a good agreement between the results of the potency test by ELISA and the results of the GP potency test. The establishment of the potency test by ELISA has made it possible to monitor the potency in the production process and has contributed to the stable production of the vaccine. 相似文献
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Masumi Yamagishi Motoyasu Otani Mariko Higashi Yoshimichi Fukuta Kiichi Fukui Takiko Shimada 《Euphytica》1998,103(2):227-234
Diallel analysis has revealed that anther culturability in rice (Oryza sativa L.) is a quantitative trait controlled by the
nuclear genome. Mapping of anther culturability is important to increase the efficiency for green plant regeneration from
microspores. In the previous study, we detected distorted segregation of RFLP markers in rice populations derived from the
anther culture of an F1 hybrid between a japonica cultivar ‘Nipponbare’ and an indica cultivar ‘Milyang 23’. To clarify the association between chromosomal
regions showing distorted segregation and anther culturability, the anther culturability of doubled haploid lines derived
from the same cross combination was examined, and the association between alleles of the RFLP markers, which exhibiting the
most distorted segregation on chromosomes 1, 3, 7, 10 and 11, and the anther culturability was evaluated. One region on chromosome
1 was found to control callus formation from microspores, and one region on chromosome 10 appeared to control the ratio of
green to albino regenerated plants. In both regions, the Nipponbare allele had positive effects. Three regions on chromosomes
3, 7 and 11, however, showed no significant effect on anther culturability.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
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Yohei Koide Daisuke Fujita Analiza G. Tagle Kazuhiro Sasaki Tsutomu Ishimaru Yoshimichi Fukuta Nobuya Kobayashi 《Euphytica》2013,192(1):97-106
Total spikelet number per panicle (TSN) is thought to be one of the most important agronomic traits associated with grain production in rice (Oryza sativa L.). We previously reported the development of 334 introgression lines (ILs) with variations in agronomic traits in the genetic background of indica rice variety IR64. Among these, an IL derived from high-yielding rice variety Hoshiaoba showed significantly higher TSN than IR64. We therefore have conducted a quantitative trait locus (QTL) analysis for TSN in an F2 population of IL × IR64 to ascertain the genetic basis for the high TSN of the IL. The analysis revealed the presence of a QTL for TSN on chromosome 7, where several QTLs for grain number have been previously reported. We developed a near-isogenic line (NIL) for this QTL by using DNA marker-assisted selection and characterized its effect. The NIL showed significantly higher TSN than IR64. These results suggest that the QTL and the NIL will be useful materials in breeding programs aimed at increasing TSN. 相似文献
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Yohei Koide Juan Pariasca Tanaka Terry Rose Ayumi Fukuo Kunihiko Konisho Seiji Yanagihara Yoshimichi Fukuta Matthias Wissuwa 《Plant Breeding》2013,132(3):259-265
Phosphorous (P) deficiency is a major yield limiting factor in rice (Oryza sativa L.) production. The interspecific New Rice for Africa (NERICA) varieties combine general stress tolerance from African cultivated rice (Oryza glaberrima Steud) with characteristics associated with high yield from O. sativa. However, little is known about their ability to tolerate P deficiency. Here, we examined the variation for tolerance to P deficiency among the 18 upland NERICAs and their parents in multi‐year field experiments. The good performance under P deficiency of the O. glaberrima parent CG 14 and some NERICAs suggested that these tolerant NERICAs contain loci associated with P deficiency tolerance inherited from CG 14. Additionally, four QTL clusters for P deficiency tolerance were detected on chromosomes 4, 6 and 11 using F3 lines derived from the cross between the P deficiency tolerant variety NERICA10 and a Japonica‐type sensitive variety ‘Hitomebore’. These QTLs represent the first step in identifying stress tolerance genes from O. glaberrima that could subsequently be used to enhance P deficiency tolerance in O. sativa. 相似文献
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