Storage of non-dormant seeds of Fraxinus angustifolia Vahl

The effects of drying and storage on the quality of Fraxinus angustifolia non-dormant seeds have been investigated. Mature seeds subjected to a 2-week warm stratification (20 °C) followed by a 2-week cold stratification (3 °C), were exposed to a gentle air stream at room temperature to dry them to 9.5% moisture content. After 4, 8 and 12 months of storage at –3 °C, in hermetically sealed containers, germination tests were conducted in darkness at 30/20 °C and 25/5 °C to evaluate possible changes in seed quality. The results obtained suggest the feasibility of drying non-dormant seeds. In fact, though causing a slight decrease in seed quality, such a process enables storage of ready to sow seeds for at least 12 months without any further loss. The temperature regime of 30/20 °C, prescribed by the International Seed Testing Association (ISTA), was found to be suboptimal for the particular conditions tested in this trial, the 25/5 °C cycle being more stimulating for germination.     

Silvicultural and economic aspects of pure and mixed native tree species plantations on degraded pasturelands in humid Costa Rica

Reforestation of degraded land in tropical regions provides one means of restoring ecosystems and improving rural livelihoods. Most plantations in humid tropical regions are established in pure plots, including few species of high commercial value, generally exotics. The present study compares growth and economic viability of 15–16 year-old trees of native species in pure and mixed plantations on degraded pasturelands at La Selva Biological Station, Costa Rica. The species with the best growth were Vochysia guatemalensis, Virola koschnyi, Jacaranda copaia, Terminalia amazonia, and Hieronyma alchorneoides. The mixed plantations performed better than pure plantations for all growth variables considered, including height, diameter at breast height, volume, and aboveground biomass. Mixed plantations outperformed pure plantations economically, with Net Present Value (NPV) of 1,124 to1,124 to 8,155/ha and Internal Rate of Return (IRR) of 7.7–15.6% depending on the species mixture. The most profitable pure plantations were those of Vochysia guatemalensis, with NPV and IRR of $6,035/ha and 14.3%, respectively; Hieronyma alchorneoides ($6,035/ha and 14.3%, respectively; Hieronyma alchorneoides (2,654 and 10.8%); and Virola koschnyi ($1,906/ha and 9.22%). These are the estimated benefits that would be accrued at harvest. These projections are realistic since the decision to harvest is generally mandated not just by profit maximization but by economic necessity, thus many small- to medium-scale farmers in Costa Rica harvest plantations prior to the completion of the rotation length. Mixed plantation may be the preferred system for reforestation with native species designed for timber production or carbon sequestration because this system is more economically viable and productive than pure plantations.     

Physiological and biochemical responses of <Emphasis Type="Italic">Dolichos lablab</Emphasis> L. to cadmium support its potential as a cadmium phytoremediator

Purpose

This study aims to investigate the response of a high biomass producer non-hyperaccumulator legume plant species, Dolichos lablab L., to cadmium (Cd) stress for phytoremediation process.

Materials and methods

Three individual experiments were carried out to assess physiological and biochemical parameters to support the use of this plant species as a phytoremediator. The first experiment was carried out in Cd-contaminated soil while the second and third experiments were conducted in sand in which Cd was applied to study biochemical responses. Analysis of mineral nutrition, phytoremediation parameters, antioxidant response, and protein identification by gel-based proteomics were performed.

Results and discussion

Good tolerance to Cd under moderate level of contamination was observed. Mineral nutrition was little affected, and phytoremediation index was satisfactory. Additionally, biochemical responses based on antioxidant enzyme analysis were well responsive in roots, reflecting the capacity of Cd stress attenuation in this organ. A proteomic analysis revealed positive regulation of root proteins involved in carbohydrate, amino acids, nitrogen metabolism, and abiotic/biotic stress response, which together may contribute to create a scenario to overcome Cd-induced stress.

Conclusions

Based on the physiological and biochemical results, we concluded that D. lablab L. is suitable for phytoremediation/phytostabilization purposes.

     

Pure and mixed forest plantations with native species of the dry tropics of Costa Rica: a comparison of growth and productivity

In Costa Rica, most reforestation trials with native species were established in the tropical humid regions. In the dry tropics, research on the performance of native species in forest plantations is incipient and trials comparing pure and mixed designs are limited. This paper presents the results of two experimental plantations with native trees in pure and mixed plots in the dry tropics of Costa Rica. The growth and productivity of 13 native species in pure and mixed plantations was compared with Tectona grandis (L.f.) Lam., an exotic species broadly used in the region. In a plantation of relatively slower growing species, measurements taken at 68 months of age resulted in Samanea saman (Jacq.) Merril. and Dalbergia retusa Hemsl. demonstrating the best growth, followed by Astronium graveolens Jacq. and Swietenia macrophylla King. Measurements in a plantation of relatively faster growing species, at 68 months of age, showed that growth of Schizolobium parahyba (Vell.) Blake was greatest in the pure and mixed plots, followed by Terminalia oblonga (Ruiz & Pav.) Steud., Anarcadium excelsum (Bert. & Balb. ex Kunth) Skeels and Pseudosamanea guachapele (Kunth) Harms. The native species grew better in the mixed plots. The pure plots of T. grandis (L.f.) Lam. were the most productive, compared to all species and the mixture of species. Plantations of T. grandis (L.f.) Lam. seem to be well adapted to the region and are certainly a commercially interesting alternative. Nevertheless, mixed plantations with native species would contribute more to sustainable management, because while single-species plantations do not provide a great range of goods and services when compared to the natural forest, mixed plantations are likely to increase this range of benefits.