Effectiveness of Protected Area Governance in the Conservation of Mt. Marsabit Forest Ecosystem,Kenya

ABSTRACT

Protected area systems (PAs) have the potential to conserve natural resources and provide social and economic benefits to local communities. Establishing and maintaining good governance and associated mechanisms is necessary for adaptive management of PAs. To test this hypothesis, we analyzed the relationships between various stakeholders and the effectiveness of PA governance of Mt. Marsabit forest ecosystem in Kenya. We used social network analysis (SNA) to assess the interactions between actors, and factor analysis to analyze the effectiveness of governance criteria. Governance of Mt. Marsabit forest ecosystem was complex with a multiplicity of stakeholders from diverse interests. Governance was moderately effective (61%) with positive indicators including the regulatory framework, delineation of areas under conservation, and reduction in poaching. The low level of interactions and associations among stakeholders suggest a weakness in the networks that may negatively affect the flow of information and other resources. This weakness was attributed to a lack of institutionalization of the links leading to poor coordination of processes. Local communities were inadequately represented in the governance of this PA despite being important actors. Our study finds that local ownership and strong linkages between actors are important ingredients for effective governance of PAs.     

Determining resistance to <Emphasis Type="Italic">Fusarium verticillioides</Emphasis> and fumonisin accumulation in African maize inbred lines resistant to <Emphasis Type="Italic">Aspergillus flavus</Emphasis> and aflatoxins

Fusarium verticillioides and Aspergillus flavus cause Fusarium ear rot (FER) and Aspergillus ear rot (AER) of maize, respectively. Both pathogens are of concern to producers as they reduce grain yield and affect quality. F. verticillioides and A. flavus also contaminate maize grain with the mycotoxins fumonisins and aflatoxins, respectively, which has been associated with mycotoxicosis in humans and animals. The occurrence of common resistance mechanisms to FER and AER has been reported. Hence, ten Kenyan inbred lines resistant to AER and aflatoxin accumulation were evaluated for resistance to FER, F. verticillioides colonisation and fumonisin accumulation; and compared to nine South African lines resistant to FER and fumonisin accumulation. Field trials were conducted at three localities in South Africa and two localities in Kenya. FER severity was determined by visual assessment, while F. verticillioides colonisation and fumonisin content were quantified by real-time PCR and liquid chromatography tandem mass spectrometry, respectively. Significant genotype x environment interactions was determined at each location (P ≤ 0.05). Kenyan inbred CML495 was most resistant to FER and F. verticillioides colonisation, and accumulated the lowest concentration of fumonisins across localities. It was, however, not significantly more resistant than Kenyan lines CML264 and CKL05015, and the South African line RO549 W, which also exhibited low FER severity (≤5%), fungal target DNA (≤0.025 ng μL^?1) and fumonisin levels (≤2.5 mg kg^?1). Inbred lines resistant to AER and aflatoxin accumulation appear to be promising sources of resistance to F. verticillioides and fumonisin contamination.     

Progress in Mapping and Cloning Qualitative and Quantitative Resistance Against <Emphasis Type="Italic">Phytophthora infestans</Emphasis> in Potato and Its Wild Relatives

Cultivated potato is susceptible to many pests and pathogens, none of which is more of a threat to potato agriculture than the late blight disease, caused by the oomycete Phytophthora infestans (Mont.) de Bary. To date all efforts to thwart this most adaptive of pathogens have failed, and early attempts to deploy ‘R genes’ introgressed from the wild Mexican hexaploid Solanum demissum ended in abject failure. With the advent of facile gene mapping and cloning, allied to knowledge of plant resistance gene structure, renewed efforts are leading to mapping and isolation of new sources of late blight resistance in potato wild species, many of which are being performed under the auspices of the BIOEXPLOIT project (Sub-project 2). We document recent advances in late blight resistance gene mapping and isolation, and postulate how these genes, allied to knowledge of pathogen effectors and their recognition specificity, may greatly enhance our chances of halting the progress of late blight disease in potato crops worldwide.     

Field evaluation of resistance to aflatoxin accumulation in maize inbred lines in Kenya and South Africa

Aflatoxin, a carcinogenic toxin, is produced mainly by Aspergillus flavus and Aspergillus parasiticus. Contamination of maize (Zea mays L.) grain by these fungi occurs before harvest, and the easiest strategy to prevent this is to develop/use maize varieties resistant to Aspergillus spp. and aflatoxin accumulation. The objective of this investigation was to identify potential sources of resistance among 23 maize inbred lines (13 obtained from the MAIZE Competitive Grants Initiative, International Maize and Wheat Improvement Centre and 10 from Agricultural Research Council, South Africa). The inbred lines were planted in a randomized complete-block design at two locations each in Kenya and South Africa. Maize ears were inoculated at silking with three toxigenic strains of A. flavus. The inoculated ears in each plot were harvested at 12–18% moisture, dried, and visually assessed for Aspergillus ear rot (AER). Aflatoxin concentration in the kernels was determined using liquid chromatography–tandem mass spectrometry. Significant variation for both AER and aflatoxin concentration existed among the inbred lines at both locations in Kenya and one location in South Africa. Combined analysis revealed a significant (p < 0.001) lines × locations interaction for both AER and aflatoxin concentration. Higher incidences of AER (0–86.0%) and aflatoxin concentration (0.21–6.51 µg/kg) were recorded at Kiboko in Kenya than at the other three locations. A stronger genetic correlation (r_G = 0.936, p < 0.0001) between the AER and aflatoxin concentration was recorded in Potchefstroom than at the other three locations. Repeatability of aflatoxin concentration was high at Kiboko (0.87) and Potchefstroom in South Africa (0.74). Three inbred lines, CML247, CML444, and CML495, emerged as potentially useful sources of resistance to AER and aflatoxin accumulation as they showed low levels of aflatoxin contamination in both localities in Kenya and in South Africa.     

Genetic parameters for large white pigs reared under intensive management systems in Kenya

A lack of performance parameters is one of the factors limiting the implementation of sustainable breeding strategies for the pig industry in Kenya. The objective of this study was to estimate genetic and phenotypic parameters for growth performance of Large White pigs reared under intensive management systems in Kenya. Growth performance data of 1398 pigs with 10 428 records were obtained from the Kenya Agricultural and Livestock Research Organization (KALRO)-Naivasha. Random regression models were used to estimate variance components by fitting different orders of Legendre polynomials. Phenotypic variance increased with age from 3.43±0.28 to 2449.28±392.07, while direct heritability ranged between 0.20±0.04 and 0.52±0.08. Maternal heritability increased from 0.26±0.05 to 0.79±0.04 while permanent environmental heritability was between 0±0.01 and 0.15±0.10. Genetic correlations were greater than 0.48 between all weights and decreased with an increase in age intervals. The first three eigenvalues of the coefficient matrix of the additive genetic covariance accounted for 98.62% of the sum of the eigenvalues. Growth was highly heritable at pre-weaning and influenced by maternal and common environmental effects. The prospect for selection for high sale weights based on pre-weaning growth performance is evident based on the high genetic correlations among body weight measurements.