Cryopreservation of Piau‐Breed Wild Boar Sperm: Assessment of Cooling Curves and Centrifugation Regimes

This study aimed to assess the effects of different cooling curves and centrifugation regimes used in cryopreservation protocols on the post‐thaw viability of Piau‐breed wild boar (Sus scrofa) sperm using in vitro assessment tests. Two centrifugations (800  g for 10 min and 2400  g for 3 min) and two cooling curves (conventional cooling using nitrogen vapour – freezing 1 and automated cooling using a programmed freezing machine – freezing 2) were tested. Therefore, the treatments were divided into M3 – centrifugation at 2400  g for 3 min and freezing 2; M10 – centrifugation at 800  g for 10 min and freezing 2; R3 – centrifugation at 2400  g for 3 min and freezing 1; and R10 – centrifugation at 800  g for 10 min and freezing 1. No significant differences (p > 0.05) between treatments occurred post‐thawing regarding the total sperm motility means recorded. The mean values of the different treatments were not different from each other regarding the supravital staining (SV), hypo‐osmotic test (HO), sperm–egg binding assay or sperm morphology. This study showed that both the cooling curve and the centrifugation regime affected the quality of post‐thaw sperm, and centrifugation for shorter times and cooling curves using automated cooling are the most suitable for minimizing sperm injury.     

森林经营中的地方分权和权力下放

一、引言 地方分权和权力下放，以及一系列相关的术语(如参与和权力)目前已被广泛用于有关林业政策的讨论中。但是这些术语过去常常被用来描述森林经营的各种方法和过程，并且常被错误地互换。本文的目的是界定这些术语和概念的含义；总结这些概念在实践中的应用方式，并依据已有的经验来指导将地方分权和权力下放这些概念应用于今后的森林经营实践。 二、定义和概念 地方分权和权力下放这两个术语常被互换，但区分二者之间的差别很重要。地方分权可以定义为：将地方的管理职能在当地进行重新分配，这并不一定意味着决策中心的改变或权力的…     

Hydraulic conductivity and embolism in the mangrove tree Laguncularia racemosa

We measured xylem pressure potentials, soil osmotic potentials, hydraulic conductivity and percent loss of conductivity (PLC) due to embolism, and made microscopic observations of perfused dye in the white mangrove tree, Laguncularia racemosa (L.) Gaertn. f., (1) to determine its vulnerability to air embolism compared with published results for the highly salt-tolerant red mangrove tree, Rhizophora mangle L., and (2) to identify possible relationships between air embolism, permanent blockage of vessels and stem diameter. Laguncularia racemosa was more vulnerable to embolism than reported for R. mangle, with 50 PLC at -3.4 MPa. Narrow stems (5-mm diameter) had higher PLC than larger stems (8.4- or 14-mm diameter) of the same plants. Basic fuchsin dye indicated that up to 89% of the vessels, especially in the narrow stems, had permanent blockage that could not be reversed by high pressure perfusion. Air embolism could lead to permanent vessel blockage and eventual stem mortality. Such vulnerability to embolism may restrict the growth of L. racemosa and limit its distribution to less salty areas of mangrove communities.     

Agroforestry systems in North America

Agroforestry systems in North America vary widely in terms of components (tree, forb, graminoid, and shrub species) and outputs. Most of the agroforestry systems used in North America have emphasized wood and livestock production. The objective of each system has been to produce annual and long term economic returns and sustainable yields. Inputs such as fossil fuels, fertilizers, herbicides and pesticides are relatively low compared to those used in conventional agriculture.Major agroforestry system types in each of eight North American regions are described. The major species used as vegetational components in each system are enumerated by region. The numerous variations in how these components are mixed have created an almost endless number of actual systems. Management problems and solutions, economic concerns, and system comparisons have also been addressed for each region.     

Haplotypes of “<Emphasis Type="Italic">Candidatus</Emphasis> Liberibacter solanacearum” suggest long-standing separation

Three haplotypes of the recently discovered bacterium species “Candidatus Liberibacter solanacearum” are described and related to geographic ranges. The first two are associated with Zebra Chip/Psyllid Yellows of potatoes and other solanaceous plants, vectored by the tomato/potato psyllid Bactericera cockerelli in North and Central America and New Zealand. The third is associated with diseased carrots in Finland and vectored by the carrot psyllid Trioza apicalis. The haplotypes are described by SNPs on the 16s rRNA, 16s/23s ISR and 50s rplJ and rplL ribosomal protein genes. These SNPs are inherited as a package across the three genes. Haplotype “a” has been found primarily from Honduras and Guatemala through western Mexico to Arizona and California, and in New Zealand. Haplotype “b” is currently known from eastern Mexico and northwards through Texas to south central Washington. These haplotypes show some range overlap in Texas, Kansas and Nebraska. The haplotypes are not yet known to elicit biological differences in the plant or insect hosts. These apparently stable haplotypes suggest separate bacterial populations of long standing.     

Evidence for the occurrence of induced resistance to pitch canker,caused by Gibberella circinata (anamorph Fusarium circinatum), in populations of Pinus radiata

Pitch canker, caused by Gibberella circinata, was discovered in California in 1986. Although initially quite damaging to Monterey pines (Pinus radiata), the severity of pitch canker has moderated in areas where the disease was first observed and some trees appear to have recovered completely. The absence of symptoms on trees that were once severely affected implies they have become more resistant to the disease. Experimental work has shown that P. radiata can manifest systemic induced resistance (SIR) in response to infection by the pitch canker pathogen and observations of disease remission may indicate that SIR is operative under natural conditions as well. As a test of this hypothesis, the susceptibility of trees in remission was assessed by inoculating them with G. circinata and recording the extent of lesion development. In addition, randomly selected trees in areas that differed in residence time of pitch canker were inoculated to determine if trees with a longer period of exposure to the pathogen were more resistant to the disease. The results of these tests showed that 89% of trees observed to be in remission sustained very limited lesion development, consistent with resistance to pitch canker. Furthermore, trees in areas where pitch canker was well established tended to be more resistant than trees in areas where the disease was of more recent occurrence. In sum, these findings support the view that SIR occurs in P. radiata and is contributing to a moderation of the impact of pitch canker under natural conditions.     

Long-distance commuting and population change in Georgia, 1960-80

The authors investigate counter-urbanization and population change in Georgia between 1960 and 1980 by examining commuting patterns. "The immediate objective is to construct the entire set of commuting fields of both large metropolitan areas to metropolitan and nonmetropolitan employment centers. We then proceed to the relationship between commuting from nonmetropolitan areas to all levels of Georgia's urban hierarchy and population change in these nonmetropolitan settings. By matching population growth and decline areas with explicit employment ties...the nature of the population changes is much better understood." Data from the 1960, 1970, and 1980 censuses for 581 Census County Divisions (CCDs) in Georgia are analyzed. It is concluded that "most nonmetropolitan growth taking place in Georgia is associated with intensification of metropolitan commuting fields along with growth of nonmetropolitan centers and their influence along the very periphery of metropolitan spheres of employment influence. However, a significant share of Georgia's nonmetropolitan population revitalization is explained by growth independent of direct metropolitan influence. It would appear then that nonmetropolitan growth centers are an important part of the basis for population decentralization in Georgia. Metropolitan spill-over alone cannot account for counterurbanization on this portion of the American economic landscape."