XX景区水体异味调查分析及对策建议

对XX景区2014年夏季高温季节发生的水体异味恶臭现象进行了调查,通过现场勘察、取样分析等方法确定了异味恶臭来源及产生原因,并提出了相应的防治对策和建议.     

铜、锌离子影响丹参金属硫蛋白MT2基因表达

植物金属硫蛋白在金属离子的储存、运输和代谢及降低重金属离子的毒性等方面起着重要作用本文研究了Cu2 、Zn2 对丹参毛状根中金属硫蛋白基因表达的影响.以6,7-V为液体培养基,分别以缺乏正常及原培养基100、500、1 000倍的cu2 、Zn2 处理,采用半定量PCR法检测不同时间点时金属硫蛋基因的表达情况.结果发现Cu2 、Zn2 诱导金属硫蛋基因的表达量分别在100倍处理量时的9 h和6 d达到峰值Cu2 、Zn2 能显著诱导丹参金属硫蛋白基因的表达,该基因可能在丹参对铜、锌元素的吸收方面发挥着重要的作用.     

桃江和浏阳病圃稻瘟病菌遗传多样性的SSR分析

2009年,在桃江和浏阳采集了26个品种上的稻瘟病标样,经单孢分离获得162个稻瘟病菌株,利用10对SSR引物进行了遗传多样性分析.结果表明,在0.80的相似水平上,162个菌株划分为23个宗谱,L01为优势宗谱,占总菌数的72.8%;在2个病圃相同品种上分离得到的59个菌株归属16个宗谱,桃江病圃的有13个宗谱,浏阳...     

乡村“活广告”赚钱有门道

在乡村做“活广告”是个好的创意。这里说的“活广告”就是有人解说,有物品展示的广告。农村的富余劳动力、下岗职工、无业人员在乡村开发经营这种“活广告”,可以说是四季赚钱、一本万利的创业新门路。     

川芎甜菜碱醛脱氢酶cDNA的克隆及其植物表达载体的构建

以川芎为试材,从叶片中提取总RNA,经过RT-FCR获得甜菜碱醛脱氢酶(Betaine aldehyde dehydrogenase)基因的cDNA,纯化后与pMD18-T载体连接,转化大肠杆菌Top10,获得甜菜碱醛脱氢酶全长基因序列,以期构建植物表达载体.结果表明:甜菜碱醛脱氢酶全长核苷酸长度为1 527 bp,编码508个氨基酸.与GenBank中已发表序列HM35276进行比较,核苷酸同源性为100％.将该基因片段克隆到植物表达载体pBI121中,构建重组质粒pBI121/Betaine aldehyde dehydrogenase,并将所获重组质粒经过双酶切和PCR处理后进行序列测定,证实表达载体上含有目的片段,且连接、构建正确,为BADH的进一步表达奠定了基础.     

粉红琵鹭雌性担主角

春暖花开时粉红琵鹭飞到江海之滨，寻找情侣，择偶产卵。其他鸟类择偶求爱大多数雄性主动，而粉红琵鹭则相反，由雌性担当主角。     

稻曲病菌不同颜色厚垣孢子超微结构比较

 The surface and internal structure difference of yellow and black dormant chlamydospores of Ustilaginoidea virens on mid-and late-season rice were examined by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). SEM revealed that the spherical yellow and black chlamydospores had prominent spines, with the diameters from 3 to 6 μm and 4 to 6 μm, respectively. The spines were 200-700 nm and 400-1 100 nm long in yellow and black chlamydospores, respectively. Variance analysis showed that there was no difference in diameter of the yellow chlamydospores but significant difference among the black ones collected from both rice cropping types. Similarly, there was no difference in length of the spines on the same color chlamydospores but extremely different between the yellow and black chlamydospores collected from the two cropping types. TEM revealed that the cell wall of both color chlamydospores were composed of two layers, the thick endosporium and thin exosporium, and the thickness of the former layer of the black chlamydospores was nearly two times as that of the yellow ones. The organelles in yellow chlamydospores such as the nucleus were obviously visible, but invisible in black ones. The cytoplasm of black chlamydospores was occupied by a big lipid globule. This study suggests that the formation of dormant chlamydospores of U. virens is related with the structural changes in the cell.     

121个水稻品种的抗瘟性鉴定

用22个稻瘟病菌株,采用离体接种的方法对121份水稻品种进行抗瘟性鉴定,同时对其中16个湖南省主栽水稻品种进行了病圃鉴定。结果表明,51个品种抗菌频率在60%以下,63个在60%～80%之间,7个品种在80%以上。湖南省16个主栽水稻品种病圃叶瘟与穗颈瘟发病率之间存在显著正相关关系,病圃叶瘟和穗颈瘟发病严重度与室内离体鉴定结果也均呈显著正相关关系。     

3株马尾松外生菌根真菌菌丝最适生长条件探究

为明确小笠原须腹菌、硬皮地星和彩色豆马勃的最适生长条件，研究温度、pH、碳源、氮源及碳氮比对3种外生菌根真菌生长的影响。结果表明3种ECM真菌的最适生长条件具有明显差异，小笠原须腹菌最适pH为4.0～5.0，最适温度为25～30℃，最适碳源为蔗糖、果糖、淀粉和甘露醇，最适氮源为铵态氮，最适碳氮比为40∶1；硬皮地星最适pH为5.0～7.0，最适温度为25℃，最适碳源为蔗糖，最适氮源为铵态氮，最适碳氮比为5∶1；彩色豆马勃最适pH为6.0，最适温度为30～35℃，最适碳源为葡萄糖，最适氮源为铵态氮、硝态氮和蛋白胨，最适碳氮比为10∶1。