基于项目的模拟电子技术课程教学模式研究

以《模拟电子技术》课程为对象,对基于项目的教学模式进行了探讨,主要提出了项目法教学的研究内容及需要解决的关键问题,并给出了具体的实施办法。     

橡胶树红根病病原菌生物学培养特性

对橡胶树红根病病原菌Ganoderma Pseudoferreum(wakef)Orver.et steinm [异名Ganoderma philippii(Bres.et.Henn)Bres.)]生物学培养特性进行研究.结果表明,菌丝生长的最适宜温度为28℃,最适pH值为6-7,该菌可有效地利用部分碳源和氮源,碳源以D-果糖和酵母浸出液最好,氮源以蛋白胨最好.光照处理对菌丝的生长有明显影响,黑暗利于菌丝生长,光照有抑制作用,黑光能杀死菌丝.菌丝的致死温度为62℃,10min.     

石梓栽培技术的研究

本文报道了石梓栽培技术研究结果：23°N以南为石梓适生地区，其年均温20．5－24．5℃，极端低温＞－1．5℃，年降雨量1200－2000mm，年平均风速＜2．5m/s。适宜立地为地形开阔，阳光充足，土壤疏松，表土层含有机质＞2％，全氮＞0．1％，速效磷＞0．025mg/100g土，PH4．5－6．9，土壤含水率10％－24％，种子贮存以干燥器最好，贮存一芽率87．4％，催芽用浸晒和浸沤处理，比不处理提前10天发芽，发芽率提高9％－12％，不同子叶类型的芽苗分别移植，较混合移植的苗木合格率提高20．6％；评定了种子主要质量指标和效果良好的育苗，造林方法。技术综合指标；材积年均生长量＞12m^3/ha。     

石梓种子的发芽试验

石梓(Gmelina arborea)种子特性与柚木相似,过去曾做过试验,在室内发芽皿中不发芽,一般贮藏方法,一年内即失去发芽力。有些生产单位播种覆土盖草,种子容易霉烂,低温季节播种,种子发芽困难。针对上述问题,我们进行了种子贮藏和催芽方法的探讨,取得了一定的效果。     

Phenolic, flavonoid, and lutein ester content and antioxidant activity of 11 cultivars of chinese marigold

This study analyzed 11 Chinese cultivars of marigold to determine their major phytochemical contents and antioxidant activities. Dried marigold flowers were extracted with ethanol, ethyl acetate, and n-hexane and the extracts were analyzed by high-performance liquid chromatography-mass spectrometry and chemical methods to determine their lutein esters, phenolic and flavonoid contents, and antioxidant activity, respectively. The different cultivars of marigold showed considerable variations in their lutein ester contents, ranging from 161.0 to 611.0 mg/100 g of flower (dry basis). The lutein esters in marigolds consisted predominantly of six all trans-diesters, but small amounts of cis isomers of the respective diesters were also present. The different cultivars of marigold also showed marked variations in total phenols and flavonoids, as well as antioxidant and radical-scavenging activities. Ethanol was confirmed to be the best solvent for extracting both phenols and flavonoids from marigold flowers, while n-hexane was the worst. The ethanolic extracts also exhibited the highest antioxidant and radical-scavenging activities. The cultivar Xinhong had the highest phenolic and flavonoid contents and radical-scavenging activity, as well as one of the highest lutein contents and antioxidant activities.     

芒果炭疽病菌环境pH信号调控基因PalF的克隆与分析

通过本地查找胶孢炭疽病菌(Colletotrichum gloeosporioides)橡胶菌株全基因组测序数据,获得PalF基因的序列信息。利用同源克隆的方法扩增胶孢炭疽病菌芒果菌株PalF基因,获得片段大小为2 369 bp的目的基因片段。对该基因序列进行分析发现,该片段含有完整的开放式阅读框,与橡胶炭疽菌PalF基因的序列同源性达100%。用RT-PCR的方法获得PalF基因的cDNA序列,序列进行分析发现该基因全长为2 310 bp,其中含有1个大小为59 bp的内含子,推测编码769个氨基酸。对PalF基因的氨基酸序列进行推测保守结构域分析可知PalF蛋白中含有Arrestin-C保守结构域,推测该基因参与环境pH感应信号转导途径(Ambient pH-sensing signal transduetion pathway)的调控,为进一步研究该基因在环境pH感应信号转导途径中的作用奠定了基础。     

橡胶树红根病病原菌rDNA-ITS序列鉴定

为了更好地对橡胶树红根病病原菌进行鉴定,从海南省和云南省发病严重的胶园采集病害样本、分离得到12个灵芝属菌株,并对其rDNA-ITS序列绘制Ganoderma属系统发育树。结果表明,采集得到的12个菌株中有11个鉴定为Ganoderma philippii、1个鉴定为G.gibbosum。