Thecoma in a mare

A 4-year-old crossbred Thoroughbred mare was referred to the University of Tehran Veterinary Medical Teaching Hospital for evaluation of a mass in the pelvic cavity. The mare had been partially anorectic and pyrexic. On clinical examination, the mare was thin and depressed, with body temperature of 38°C, a pulse rate of 38 beats/minute, and a respiratory rate of 10 breaths/minute. Palpation per rectum revealed a large, smooth and tense mass in the anterior part of the pelvic cavity. Ultrasound examination confirmed the presence of a soft tissue mass with a thick wall. The inner surface of the mass had a villous-like appearance. The stroma of the mass was mottled, with accumulation of speckled and hypoechoic to slightly hyperechoic fluid. Hormone assays revealed low serum concentration of testosterone. Serum progesterone concentration indicated that active luteal tissue and serum estradiol concentration was 24 pg/ml. On histological examination, the sections from the tumor were composed of oval- or spindle-shaped cells loosely arranged in diffuse sheets or irregularly interlacing fascicles. On the basis of these histological findings, the large tumor mass of the left ovary was considered to be a thecoma. To our knowledge, the details of the clinical, ultrasonographic, endocrinologic, and histologic findings of this tumor in the mare have not been described in the veterinary literature.     

Efficiency of some molecular markers linked to rhizomania resistance gene (Rz 1 ) for marker assisted selection in sugar beet

Sugar beet (Beta vulgaris L.) is one of the two major products supplying sugar (sucroses) in the world. Rhizomania is one of the most destructive diseases of sugar beet world-wide. Holly is the major source of resistance to rhizomania. The objectives of this study were to identify the dominant homozygous genotypes resistant to rhizomania using ZN1 molecular marker, to field evaluate S₁ progenies of plants already proved to be containing the marker and also to determine the relationship of this and other SCAR (sequence characterized amplified region) markers with SNP1 (single nucleotide polymorphism) marker associated with the Rz ₁ gene. Molecular analysis was carried out on 27 O-type populations (consisting of 13 susceptible and 6 resistant genotypes). Field evaluation and scoring of the phenotypic traits including greenness, growth, uniformity and disease score of 12 O-type populations were carried out on a rhizomania-infested field. The percent agreement of coupling marker ZN1 and repulsion marker ZN8 with disease score was 0.91 and 0.93, respectively. Although all O-types had the Rz ₁ resistance gene but the phenotypic differences were observed due to the effect of different genetic backgrounds and modifier genes. Overall, the results showed that the selected markers can be used for marker-assisted selection (MAS) to reduce the time and cost of breeding programs and increase the efficiency of selection.     

Serum biochemistry and hematology values and hemoglobin electrophoresis in Persian squirrels (Sciurus anomalus)

BACKGROUND: Serum biochemical and hematologic parameters are important in the management of game species in Iran, such as Persian squirrels. OBJECTIVE: The purpose of this study was to establish baseline serum chemistry and hematology values in Persian squirrels (Sciurus anomalus) and describe blood cell morphology and the electrophoretic pattern of hemoglobin. METHODS: Blood samples were collected from 30 Persian squirrels (Sciurus anomalus) maintained in captivity in the Tehran Zoo. Blood was placed into EDTA and serum clot tubes and analyzed using standard manual hematology and biochemical techniques. Hemoglobin electrophoresis was done on cellulose acetate paper strips. RESULTS: Minimum, maximum, and median values were obtained for 11 hematologic and 12 serum chemistry parameters. Hypersegmented neutrophils were observed frequently. We did not find basophils or band neutrophils. Hemoglobin electophoresis resulted in a band slightly anodal to that of human hemoglobin A. CONCLUSION: Biochemical and hematologic values in Persian squirrels were comparable to those of related species, and may be used as a standard profile for healthy Persian squirrels kept in captivity.     

Molecular progress in sugar beet breeding for resistance to biotic stresses in sub-arid conditions-current status and perspectives

The yield of sugar beet depends mainly on the effective protection to a number of pests and diseases affecting the crop. In the absence of actual methods of management, as in the case of viral diseases, the availability of genetic resistance sometimes allows the survival of the crop in the affected areas. Integration of molecular markers in conventional breeding procedures has provided a reliable means for improving the efficiency of selection methods. The present review summarizes the evolution, thanks to the application of molecular techniques, of traditional breeding for resistance to some biotic stresses in sub-arid environments.     

Treatment of serous retinal detachments associated with optic disk pits in dogs

Serous retinal detachment, associated with optic disk pit, was diagnosed in 28 eyes of 24 dogs. Xenon arc photocoagulation was used in treatment of the detachment. Of 24 dogs, 21 were Collies. In 23 eyes, retinal detachments affected temporal and/or inferior portions of the retina. In 5 eyes, detachments were predominantly superior and/or nasal. A single photocoagulation treatment resulted in reattachments in 25 eyes. Of the 3 remaining detachments, 2 eyes improved with additional photocoagulation, and 1 eye, which was not treated further, had a complete retinal detachment.     

Weed Control Efficacy: Response of Chickpea to Pre- and Post-Emergence Herbicides

Lack of control options for cool-season broadleaf weeds is a major deterrent to autumn-sown chickpea. Weed control and chickpea tolerance to PRE (pre-emergence) and POST (post-emergence) application of isoxaflutole and oxyflurofen, PRE metribuzin, POST pyridate, and flumetsulam were investigated at three locations, including Kermanshah, Kurdistan, and Hamedan provinces during 2017–2018. Untreated and weed-free checks were added for comparison. Pyridate and PRE oxyflurofen 125?g ai ha^?1 caused the minor visual crop injury according to EWRS score (1–1.8), while the highest crop injury occurred with metribuzin (EWRS score 3.5–8.5) in whole locations. The most effective herbicides for weed reduction were pyridate (70–75%), PRE oxyfluorfen (69–76%), and POST oxyfluorfen (65–73%) at Kermanshah, PRE oxyfluorfen at 125 and 175?g ai ha^?1 (70–78%), POST oxyfluorfen (70–76%) and pyridate (70–78%) at Kurdistan, PRE oxyfluorfen at 125 and 175?g ai ha^?1 (88–96%), metribuzin (91–100%) and Pyridate (80–97%) at Hamedan. Pyridate and PRE oxyfluorfen at 125?g ai ha^?1 resulted in the highest chickpea grain yield at the three locations. In general, PRE oxyfluorfen (125?g ai ha^?1) was similar to pyridate in terms of efficacy in weed control and grain yield enhancement.

     

Enhanced resistance to a lepidopteran pest in transgenic sugar beet plants expressing synthetic <Emphasis Type="Italic">cry1Ab</Emphasis> gene

Two diploid sugar beet genotypes of agronomical importance were transformed using Agrobactrium tumefaciens harboring pBI35Scry containing a synthetic cry1Ab gene. Leaf blade with attached shoot bases, a highly regenerative tissue, were used as explant substratum for transformation. PCR screening with cry1Ab-specific primers showed the presence of transgene in more than 50% of the regenerated kanamycin-resistant plants after treatment with the antibiotic. A transformation rate of 8.8–12.2% (depending on genotype) was achieved as revealed by genomic DNA dot blotting. The intact integration of transgene cassette into the genome was furthermore confirmed by Southern blot analysis. The expression of the cry1Ab gene encoding a truncated endotoxin (67 kDa) at about 0.1% of total soluble protein was achieved in the leaves of transgenic plants as shown by Western blot analysis. Bioassays under in vitro conditions with Spodoptera littoralis, one of the most important pests in sugar beet fields, demonstrated enhanced resistance against this pest. The inheritance of the inserted transgene was confirmed in F₁ plants obtained through crossing of T₀ plants with a cytoplasmic male sterile line. Transgenic plants are currently grown in a greenhouse and will be subjected to further bioassay analyses against other lepidopteran pests of sugar beet.     

Spatial attenuation of ambient particulate matter air pollution within an urbanised native forest patch

Of interest to researchers and urban planners is the effect of urban forests on concentrations of ambient air pollution. Although estimates of the attenuation effect of urban vegetation on levels of air pollution have been put forward, there have been few monitored data on small-scale changes within forests, especially in urban forest patches. This study explores the spatial attenuation of particulate matter air pollution less than 10 μ in diameter (PM₁₀) within the confines of an evergreen broadleaved urban forest patch in Christchurch, New Zealand, a city with high levels of PM₁₀ winter air pollution. The monitoring network consisted of eight monitoring sites at various distances from the edge of the canopy and was operated on 13 winter nights when conditions were conducive for high pollution events. A negative gradient of particulate concentration was found, moving from higher mean PM10 concentrations outside the forest (mean=31.5 μg m^?3) to lower concentrations deep within the forest (mean=22.4 μg m^?3). A mixed-effects model applied to monitor meteorological, spatial and pollution data indicated temperature and an interaction between wind speed and temperature were also significant (P?0.05) predictors of particulate concentration. These results provide evidence of the potential role that urban forest patches may play in mitigating particulate matter air pollution and should be considered in plans for improving urban air quality.