排序方式: 共有37条查询结果,搜索用时 15 毫秒
1.
2.
剪股颖粒线虫[Anguina agrostis (Steinbuch,1799) Filipjev,1936]、小麦粒线虫[Anguina tritici (Steinbuch,1799) Filipjev,1936]和维氏粒线虫[Anguina wevelli (Van den Berg,1985) Siddiqi,2000]都是重要的植物病原线虫,它们在成熟种子和虫瘿中的虫态通常是幼虫,而这3种线虫的幼虫形态非常相似,难以根据其特征对它们进行快速准确的种类鉴定。本研究根据这3种线虫的rDNA-ITS区域序列,分别设计筛选了特异性引物AgrF1/AgrR1、TriF1/TriR1、WevF1/WevR1,构建了这3种线虫单条幼虫多重PCR检测体系,获得3个大小差异明显的片段,表明这些引物设计合理,适合这3种线虫的快速准确检测。 相似文献
3.
4.
Objective To evaluate the antituberculous activities of Huangkuisu (HISS). Meth-ods lOStandard human strains of mycobacterium tuberculosis (H37RV) and multi-drug resistant TBstrainswere inoculated in different concentrations of HISS, blank control and positive TB drug culture medium[()observe the growth of mycobacterium tuberculosis and evaluate the antibacterial activity in vitro.②Certain number of standard strains of Mycobacterium tuberculosis H37Rv and drug-resistant strains were injected into the tail vein[()establish a mouse model of mycobacterium tuberculosis infection. The mice were randomly divided into treatment group, rifampicin, HISS treatment group treated lasting for 6 weeks; the control group was given normal saline. The antibacterial activity in vivo were evaluated[()compare the treatment results according[()the pathological changes in lung and spleen tissues (HE staining and acid-fast staining) and charging amount of bacteria. Results lOthe bactreial colonies were grew normally in model group, but were not grew in 相似文献
5.
本文对国内外研究者设计的11对PCR引物检测柑桔黄龙病菌的特异性进行了比较。其中包括5对一步法PCR引物(A2/J5、GB1/GB3、MP1/MP2、CQULA03F/CQULA03R和CLA-F2/CLA-R2)和6对巢式PCR引物(P1/P2、P3/P4、LP1/LP2、LP3/LP4、DP1/DP2和DP3/DP4)。结果发现,MP1/MP2和CLA-F2/CLA-R2 2对引物具有非特异性,不能区分亚洲柑桔黄龙病菌和非洲柑桔黄龙病菌,其余的9对引物特异性较好。本文旨在为研究者选择柑桔黄龙病菌扩增引物提供参考。 相似文献
6.
7.
8.
9.
深圳口岸旅检截获香蕉穿孔线虫 总被引:2,自引:0,他引:2
2003年6月11日,罗湖局检疫人员在旅检时发现一旅客携带一盆红掌苗,按规定给予截留. 相似文献
10.