动物细胞遗传学研究现状及其应用

本文介绍了细胞遗传学在动物方面的研究现状及其在畜牧业上的应用。细胞遗传学的研究主要以染色体为对象。目前世界上现存的哺乳动物约4100多种,其中进行过染色体研究或核型分析的有1468种,我国已研究过的有242种。细胞遗传学在人类医学上的应用是众所周知的,在畜牧业上主要用于对种畜进行细胞学检查,以消除对家畜繁殖性能有影响的畸变染色本,以染色体标记来识别家畜品种,对环境污染的监测及遗传操作中的染色体工程。     

Male-to-female aggression in cage-housed common pheasants (Phasianus colchicus) during the breeding season was not related to male plasma testosterone level

1. The aim of this study was to investigate if male-to-female aggression of common pheasants in the course of the breeding season was related to the concentration of plasma testosterone and/or other biochemical plasma indicators in male pheasants housed in breeding cages. The influence of season on the concentration of testosterone and biochemical indicators was also investigated.

2. Males were divided into non-aggressive and aggressive groups during the breeding season based on ethological evaluation. At the beginning, in the middle and at the end of the breeding season, a blood sample was taken from all males on the same day and the concentration of selected biochemical indicators and the total circulating testosterone in the plasma were determined.

3. Male-to-female aggression during the breeding season of pheasants was not influenced by the total plasma testosterone of males.

4. The concentration of total plasma testosterone in males decreased gradually during the breeding season.

5. Male-to-female aggression of pheasants did not have a significant effect on any of the assessed biochemical indicators.

6. The influence of the breeding season affected the activities of alanine aminotransferase and aspartate aminotransferase as well as the concentrations of glucose, magnesium, potassium and chloride in the blood plasma of cage-housed male pheasants.     

Gas and short‐chain fatty acid production from feeds commonly fed to red deer (Cervus elaphus L.) and incubated with rumen inoculum from red deer and sheep

Efficient red deer supplementary feeding depends on estimations of the nutritive value of offered feeds, frequently estimated with the use of equations derived from domestic ruminants. The aim of this study was to compare the 24‐hour in vitro true dry matter degradability (ivTD₂₄), in vitro gas production (GP) kinetic parameters, GP in 24 hr of incubation (GAS₂₄) and short‐chain fatty acid (SCFA) and microbial biomass (MBS) produced after 24‐hour incubation of feeds in inoculum prepared from sheep and red deer rumen fluid. Eleven feeds, frequently consumed by red deer in Slovenia, which occur either naturally (two fresh grasses, chestnut fruits and common and sessile oak acorns) or are fed as winter supplemental feeds (two grass hays, two grass silages, apple pomace, fresh sugar beetroot), were investigated. The in vitro GP kinetic parameters, GAS₂₄ and ivTD₂₄, did not differ between animal species. Amounts of SCFAs were greater (p < 0.05) when feeds were incubated in sheep inoculum, while molar proportions of acetic and propionic acids did not differ. Molar proportions of butyric acid produced during incubation of high fibre feeds did not differ between animal species, but were higher (p < 0.05) when feeds high in starch or sugar were incubated in red deer inoculum. Greater production of SCFA by sheep rumen microbes suggests better coverage of host animal with energy precursors, while greater production of MBS by red deer rumen microbes suggests better coverage of host animal with protein. Results also suggest that rumens of sheep and red deer are inhabited by different microbial communities, which did not affect the extent of in vitro GP and degradation of feeds used in the present experiment. However, the possibility exists that the divergent nutrient use could be a consequence of different priming by different feeds of the donor animal diets.     

Sex‐dependent responses of perch to changes in water clarity and temperature

Rising temperatures and decreasing water transparency of lakes have strong wide ranging effects on fish. Fish responses to various changes in the environment are usually species‐dependent, but responses may also vary within species. In general, large individuals are considered to be more sensitive to environmental variation due to higher energy demand, than smaller individuals. Similarly, large individuals require more food to maintain bodily functions and are thus more sensitive to resource and food scarcity. These size‐specific responses to environmental gradients are also sex‐dependent in species that exhibit sexual size dimorphism (SSD). We studied in enclosures with short‐term experiments how rising temperatures and decreasing water transparency regulate the feeding rates of female and male European perch (Perca fluviatilis L.). To explore experimental results, we calculated perch SSD in nine lakes with varying environmental conditions using previously collected field data. The results of the experiments revealed that the combined effect of water transparency and temperature on the feeding rate of fish is gender‐dependent: feeding rate of females decreased more than that of males. The experimental results were also supported by field data that revealed a negative relation between water transparency and the magnitude of SSD in perch. Our results suggest that rising temperatures and decreasing water transparency may potentially decrease fish size in a sex‐dependent manner. As female size is one of the main demographic traits determining the reproductive success of a fish population, changing environments may have unexpected and far‐reaching consequences on fish population dynamics.     

Physiological Response of Multiple Contrasting Rice (Oryza sativa L.) Cultivars to Suboptimal Temperatures

The physiological response of multiple rice cultivars, eighteen initially and eight cultivars later on, to suboptimal temperatures (ST) conditions was investigated in laboratory and outdoor experimental conditions. Treatment with ST decreased growth in different extents according to the cultivar and affected the PSII performance, determined by chlorophyll fluorescence fast‐transient test, and stomatal conductance, regardless the experimental condition. Two groups of cultivars could be distinguished on the base of their growth and physiological parameters. The group of cultivars presenting higher growths displayed optimal JIP values, and higher instantaneous water use efficiency (WUE_i), due to a lower Gs under ST, unlike cultivars showing lower growth values, which presented worse JIP values and could not adjust their Gs and hence their WUE_i. In this work, we detected at least two cultivars with superior tolerance to ST than the cold tolerant referent Koshihikari. These cultivars could be used as parents or tolerance donors in breeding for new crop varieties. On other hand, positive and significant correlations between data obtained from laboratory and outdoor experiments suggest that laboratory measurements of most of the above mentioned parameters would be useful to predict the response of rice cultivars to ST outdoor.     

Associations of fish with various types of littoral habitats in reservoirs

Fish associations with different types of littoral habitats were studied in four canyon‐shaped reservoirs in the Czech Republic in years 2010 and 2011 by gillnets. Two to three habitats per reservoir–beaches (former meadows), stump fields (former forest) and rubble slopes–were defined and sampled along the longitudinal axis of reservoirs. Effects of reservoir, habitat and locality (position along longitudinal axis) on fish biomass, abundance and species structure were tested for juvenile and adult fish separately. Hierarchical analysis of variance revealed that habitats differed significantly in fish biomass and abundance. Redundancy analysis showed that analysed environmental variables had significant influence on fish community structure. Most variability in community structure was explained by reservoir and then by combination of habitat and slope steepness. Locality position had the smallest influence on community structure. For both adult and juvenile fish total abundance and biomass, the most inhabited habitat was beaches; rubble slopes were the least inhabited. Habitat associations differed among species. Among adults, bream Abramis brama, white bream Blicca bjoerkna and roach Rutilus rutilus were associated with beaches and stump fields, whereas perch Perca fluviatilis, ruffe Gymnocephalus cernuus, asp Aspius aspius and pike Esox lucius were associated with rubble slopes. Bream, white bream, bleak Alburnus alburnus, roach, ruffe and pikeperch Sander lucioperca were associated with beaches among juveniles, whereas the only juvenile associated with rubble slopes was perch. We showed that most common species are associated with distinct habitats and also that utilisation of various littoral habitats differs in general.     

基于PLC的碟式太阳自动跟踪控制系统研究

由于太阳辐射方向的时刻改变和太阳辐射能的不稳定性,导致碟式太阳能发电系统利用率始终较低。为了解决这个问题,本文提出了一种基于PLC控制的全天候自动跟踪方式,利用光照强度与光照强度阈值的差值作为跟踪方式切换的判据,不仅消除了程序跟踪造成的累积误差,而且避免了光电传感器在阴天或多云时无法工作的问题。为保证系统在任何天气情况下均可稳定运行,信息反馈元件增加了风速传感器、温度传感器及倾角传感器。另外,在设计系统硬软件时,采取了一系列措施来进一步降低系统运行成本。     

Comparison of Chemical Composition and Biological Properties of Essential Oils Obtained by Hydrodistillation and Steam Distillation of Laurus nobilis L.

Plant Foods for Human Nutrition - The purpose of this study was to compare the yield, chemical composition, antimicrobial and antioxidant properties of essential oils isolated from leaves of Laurus...     

Characterization of common bean wild populations for their in situ conservation in Northwestern Argentina

In situ conservation of wild species is a method of conservation that allows keeping populations in their natural environments, and set the strategies for maintaining the natural populations. The Active Bank of Northwestern Argentina (BANOA) is in charge of the in situ conservation of wild populations of common bean (Phaseolus vulgaris L.) in Northwestern Argentina (NOA), and has an ex situ collection of 401 landraces and 221 wild accessions from the NOA. We evaluated the phenotypic diversity of 68 common bean wild populations from the NOA both in protected and unprotected areas, finding a moderate variation among them. Ten phenotypic reproductive characteristics related to pod and seed displayed significant differences in the analysis of variance; these traits together with the seed weight were the basis for the multivariate analysis. The cluster analysis ordered the populations in 12 groups but trends in geographical distribution or phenotypical variation were not recognized. For the conservation in situ of the wild bean populations, their diversity should be considered. Two types of populations can be highlighted: (i) candidates for in situ conservation in order to preserve the novel variation generated by convergence with cultivated sympatric germplasm (populations 433, 437, 471, 509, 513 and 517) and (ii) those whose phenotype represents clearly the wild status and should be preserved in situ as such in their current status (populations 480, 495, 496, 525 and 533).