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Longitudinal analysis of allergen‐specific IgE and IgG subclasses as potential predictors of insect bite hypersensitivity following first exposure to Culicoides in Icelandic horses 下载免费PDF全文
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Strasser A May B Teltscher A Wistrela E Niedermüller H 《Veterinary immunology and immunopathology》2003,94(3-4):113-121
A decline in T-cell-mediated immunity and transient state of immunosuppression after immunization has been reported in dogs. Nevertheless, dogs are still routinely vaccinated with polyvalent live vaccines and severe disease does not generally occur. In order to investigate these effects on the canine immune system and to elucidate possible mechanisms we determined the following immune parameters in the blood of 33 clinically sound German shepherd dogs before and after standard vaccination with a polyvalent vaccine against distemper, parvovirus, viral hepatitis, leptospirosis, kennel cough and rabies: white and differential blood cell count, the serum concentrations and/or activities of IL-1, IL-2, IFN-gamma, TNF-alpha, neopterin and IgG, natural killer (NK) cell activity, bactericidal activity and complement hemolytic activity, lymphocyte proliferation test (LPT) and nitroblue tetrazolium test (NBT).Our major findings were that significant postvaccinal decreases in T-cell mitogenic response to PHA and in neutrophil function and neopterin serum concentration were accompanied by simultaneous increase in plasma IgG and hemolytic complement activity. This suggests a transient shift in the balance between cell-mediated and humoral (T(H)1/T(H)2) immunity rather than immunosuppression.These results do not imply that dogs should not receive live vaccines, as the response to vaccines just seems to create a state of altered homeostasis when immunization elicits protection by humoral and cell-mediated immunity. However, these recognized compromises of immune function should be considered and vaccines still be applied only in healthy animals and strictly according to the rules and regulations given by the manufacturer. 相似文献
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Based on 120 stem discs collected during3 months of fieldwork along a 12 km route,the history of fires in the Wari Maro Forest(09 1000 N–02 1000E) over the past century in savanna woodland and dry forest was reconstituted.Three major ecological areas are characterized:one highly burnt zone located between two relative less burnt areas.By analyzing tree rings,246 fire scars were identified.The scars were caused by 51 fire years,occurring at a mean interval of 2.23 years.From 1890 to1965,only 6 years with fires were recorded from sampled trees.Since 1966,no year has passed without fire.The fire frequency point scale reached 14 years.This was the case of Burkea africana,which has been identified as a species tolerant to fire and could be planted to create a natural firewall.In contrast,Anogeissus leiocarpa is highly sensitive to fire,and in a dry forest ecosystem that burns seasonally,it requires a special conservation plan.Two new concepts are described:the rebarking of trees after fire and Mean Kilometer Fire Interval.The first concept was tested with Daniellia oliveri(Rolfe) Hutch & Dalz trees,and the second concept was used to evaluate spatial fire distribution.We demonstrate that savanna woodland and dry forest were subject to a degradation process caused by destructive fires related to vegetation cover clearance and illegal logging. 相似文献
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Bettina Heilmann Michael Lebuhn Friedrich Beese 《Biology and Fertility of Soils》1995,19(2-3):186-192
Samples from a sandy agricultural soil were treated with increasing amounts of a fungicide (Sportak). The effects on the soil microflora were investigated over several weeks by monitoring basal and substrate-induced respiration and basal and substrate-induced heat output. The microbial biomass, metabolic quotient (qCO2), relative heat output (rqheat), lag phase of substrate use, and calorimetric: respirometric ratio were used as ecophysiological parametèrs. As structural and community-specific parameters, we recorded tryptophan contents and auxin metabolism, and calculated the ratios of fungal to bacterial respiration by antibiotic inhibition of substrate-induced respiration. Sportak either inhibited or stimulated the microbiota, depending on the length of exposure to the fungicide and the amount applied. Mineralization of dead biomass was reflected in increased soil tryptophan contents after the Sportak application. A shortened lag phase demonstrated inhibition and a prolonged lag phase stimulation of substrate use. This changed with the experimental phase. The rqheat and the calorimetric: respirometric ratio proved to be suitable parameters for the detection of stress metabolism (repair processes) in soil microbiota, because thermodynamic processes and catabolic and anabolic metabolism are taken into account at the same time. Following the application of Sportak, indole 3-acetic acid biosynthesis decreased while indole-3-ethanol biosynthesis increased, probably as a result of a transitional community shift from K-strategists towards r-strategists. It was not the fungicide but the formulation (mainly xylol) that damaged the organisms. A shift in the ratio of fungi to bacteria was also observed, suggesting that the bacteria were probably more sensitive to xylol than the fungi. 相似文献
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Understanding carbon dynamics in soil is the key to managing soil organic matter. Our objective was to quantify the carbon dynamics in microcosm experiments with soils from long-term rye and maize monocultures using natural 13C abundance. Microcosms with undisturbed soil columns from the surface soil (0-25 cm) and subsoil (25-50 cm) of plots cultivated with rye (C3-plant) since 1878 and maize (C4-plant) since 1961 with and without NPK fertilization from the long-term experiment ‘Ewiger Roggen’ in Halle, Germany, were incubated for 230 days at 8 °C and irrigated with 2 mm 10−2 M CaCl2 per day. Younger, C4-derived and older, C3-derived percentages of soil organic carbon (SOC), dissolved organic carbon (DOC), microbial biomass (Cmic) and CO2 from heterothropic respiration were determined by natural 13C abundance. The percentage of maize-derived carbon was highest in CO2 (42-79%), followed by Cmic (23-46%), DOC (5-30%) and SOC (5-14%) in the surface soils and subsoils of the maize plots. The percentage of maize-derived C was higher for the NPK plot than for the unfertilized plot and higher for the surface soils than for the subsoils. Specific production rates of DOC, CO2-C and Cmic from the maize-derived SOC were 0.06-0.08% for DOC, 1.6-2.6% for CO2-C and 1.9-2.7% for Cmic, respectively, and specific production rates from rye-derived SOC of the continuous maize plot were 0.03-0.05% for DOC, 0.1-0.2% for CO2-C and 0.3-0.5% for Cmic. NPK fertilization did not affect the specific production rates. Strong correlations were found between C4-derived Cmic and C4-derived SOC, DOC and CO2-C (r≥0.90), whereas the relationship between C3-derived Cmic and C3-derived SOC, DOC and CO2-C was not as pronounced (r≤0.67). The results stress the different importance of former (older than 40 years) and recent (younger than 40 years) litter C inputs for the formation of different C pools in the soil. 相似文献
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András Cseri Mátyás Cserháti Maria von Korff Bettina Nagy Gábor V. Horváth András Palágyi János Pauk Dénes Dudits Ottó Törjék 《Euphytica》2011,181(3):341-356
In the present study, allele mining was conducted on a panel of drought related candidate genes in a set of 96 barley genotypes
using EcoTILLING, which is a variant of the targeting induced local lesions in genomes (TILLING) technology. Analyzing approximately
1.5 million basepairs in barley a total number of 94 verified unique haplotypes were identified in 18 amplicons designed for
9 genes. Overall, 185 single nucleotide polymorphisms (SNPs) and 46 insertions/deletions (INDELs) were detected with a mean
of 1SNP/92 bp and 1INDEL/372 bp genomic sequence. Based on overlapping haplotype sequences, markers were developed for four
candidate genes (HvARH1, HvSRG6, HvDRF1, HVA1), which allows distinguishing between the main haplotypes showing either differences in amino acid sequence or which have
larger INDELs in the promoter region. As “proof of concept”, the HvARH1 and HvSRG6 haplotypes were tested for the level of abscisic acid-induced gene expression in subsets of genotypes belonging to different
haplotype categories. An integrated database was developed to contain information about the genes, genotypes, and haplotypes
analyzed in this study. The database supplies profound information about the natural variation in the tested drought related
candidate genes providing a significant asset for further mapping studies dealing with this highly polygenic trait. 相似文献
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Aroma quality differentiation of pyrazine derivatives using self-organizing molecular field analysis and artificial neural network 总被引:2,自引:0,他引:2
Klocker J Wailzer B Buchbauer G Wolschann P 《Journal of agricultural and food chemistry》2002,50(14):4069-4075
The encoding of various aroma impressions and the distinction between different aroma qualities are unsolved problems, as differences between aroma impressions can be described only in a qualitative but not in a quantitative manner. As a consequence, classifications of various aroma qualities cannot easily be performed by standard QSAR methods. To find a proper way to encode aroma impressions for SAR studies, a total of 50 pyrazine-based aroma compounds showing the aroma quality of earthy, green-earthy, or green are analyzed. Special attention is thereby turned on the mixed aroma impression green-earthy. Classifications on the whole data set as well as on smaller subsets are calculated using self-organizing molecular field analysis (SOMFA) and artificial neural networks (ANNs). SOMFA classifies between two or three aroma impressions, leading to models satisfying in predictive power. ANN analysis using multilayer perceptron network architecture with one hidden layer and nominal output as well as genetic regression neural network) with two hidden layers and numerical output both lead to a rather good performance rate of 94%. 相似文献
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Desjardins I Theoret C Joubert P Wagner B Lavoie JP 《Veterinary immunology and immunopathology》2004,101(3-4):133-141
Airway remodeling may play an important role in heaves pathophysiology. Transforming growth factor-beta 1 (TGF-beta1) is a potent profibrotic cytokine, which might contribute to airway wall thickening and fibrosis of bronchiolar and alveolar submucosa. An ELISA designed for the measurement of human TGF-beta1 was used to measured total TGF-beta1 released in bronchoalveolar lavage fluid (BALF) of normal horses and of those affected with heaves in remission. The specificity of the assay for TGF-beta1 of the horse was confirmed using recombinant equine TGF-beta1. The influence of hay exposure on TGF-beta1 release in the airways was also examined by stabling horses in a dusty environment. TGF-beta1 was found in the BALF of all horses. However, no significant difference between basal concentration of TGF-beta1 in BALF of control horses versus that of horses affected with heaves was found. Furthermore, no differences were identified in these populations 1 and 9 days after allergen challenge. In conclusion, these data indicate that TGF-beta1 is released in BALF fluid of horses in biologically active concentrations. Other studies are necessary for a better definition of the role of this cytokine within the lung, as our study does not establish a causal relationship between TGF-beta1 and the pathophysiology of heaves in the horse. 相似文献