Molecular characterisation of Theileria equi and risk factors associated with the occurrence of theileriosis in horses of Punjab (Pakistan)

Theileria equi (T. equi) is an obligate intra- and extra-erythrocytic parasite that causes equine theileriosis (ET) in equids. Equine theileriosis is considered a notifiable disease of global significance, a major constraint to the international movement of horses, and endemic in many countries. This disease may be difficult to diagnose, as it can produce variable and nonspecific clinical signs. A cross-sectional study was designed for the molecular characterisation of T. equi and to investigate the associated risk factors of ET accompanied by its consequences on haematological and sero-biochemical parameters. A convenience sampling of 500 blood samples were collected from ET suspect horses from January to December 2017. PCR was performed on all blood samples targeting the 18S rRNA gene of T. equi followed by sequencing; 9% animals tested positive with confirmed sequences. The isolates of this study showed high homology with Cuban, Russian and Brazilian isolates of T. equi (accession numbers KY111762.2 , MG551915.1 and KY952237.1 , respectively). Based on multivariate analysis, the principal risk factors consisted of absence of dogs on the premises and presence of tick infestation. The haemato-biochemical parameters showed a decrease in granulocytes and erythrocytes, and an increase in lymphocytes, monocytes, mean corpuscular volume, mean corpuscular haemoglobin, mean platelet volume, glucose, phosphorus and aspartate aminotransferase in positive horses. This is the first study which identified ET in Punjab (Pakistan) using molecular techniques and risk factors together with the haemato-biochemical variations in horses.     

Concentration of selected heavy metals in spices,dry fruits and plant nuts

Different spices, dry fruits and plant nuts commonly consumed in Pakistan were assayed for the heavy metals cadmium, lead, copper, zinc, iron and manganese by the potentiometric stripping analysis and AA spectrophotometry. The results revealed wide variation in heavy metal content among different biological materials. Mixed spices generally exhibited higher value for trace metals specially lead (6.6–9.2 µg/g), cadmium (0.65–1.34 µg/g), iron (142.3–285.0 µg/g) and zinc (64.2–65.8 µg/g). Dry fruits contained relatively lesser amounts of heavy metals than plant nuts. Almonds contained higher levels of lead (1.02 µg/g) and cadmium (0.24 µg/g) than other nuts and dry fruits.     

Rinderpest in the landhi dairy colony

Earlier diagnostic reports of rinderpest in buffaloes in the Landhi Dairy Colony were discounted by most veterinarians in Pakistan. Four recent investigations have shown beyond doubt that the affliction was indeed rinderpest.     

Identification of ticks and detection of blood protozoa in friesian cattle by polmerase chain reacton test and estimation of blood parameters in district Kasur,Pakistan

The study was carried out to detect Theileria annulata, the causative agent of theileriosis, and Babesia bovis, the causative agent for babesiosis, in Friesian cattle by PCR and conventional blood smear examination. One hundred blood samples obtained from diseased Friesian cattle kept on private livestock farms at Pattoki, District Kasur, Pakistan were collected in addition to 20 blood samples obtained from non-diseased animals. The disease manifestations observed clinically included high fever, swelling of sub mandibular and sub scapular lymph nodes, weakness, increased respiration and pulse, anorexia, loss of condition and rough hair coat. Neurologic sign of in coordination was also seen in weak animals. Signs of lacrimation, pale conjunctiva, diarrhoea, dyspnea and frothy nasal discharge were observed in only one animal. Clinically nine animals showed signs of haemoglobinuria. Diagnosis of bovine theileria and babesia species was based on finding many intraerythrocytic piroplasms of both blood protozoa with clinical signs associated with anaemia, lymph node hyperplasia and haemoglobinuria. One hundred samples of ticks were also collected for identification of vector. Results showed that the prevalence of Hyalomma tick was highest (15%) followed by Boophilus (12%), Haemaphysalis (5%) and Rhipicephalus (3%). The blood smear examination showed 21% (21/100) samples positive for blood parasites out of which 66.6% (14/ 21) samples were positive for theileriosis while 42.8% (9/21) were positive for babesiosis. It was also recorded that 66.66% (6/9) samples were positive for B.bigemina while 33.33% (3/9) were positive for B.bovis. The results showed that 60% (60/100) samples were positive for blood parasites by PCR test. Out of these 60% (36/60) were positive for T.annulata while 33.33% (20/60) were positive for babesia. The specificity and sensitivity of PCR test was higher than blood smear examination. The blood parameters in haemoparasites infection were also analyzed and the results showed significant decrease in total erythrocyte count and haemoglobin while MCV, MCH values increased and MCHC was slightly less than normal indicating macrocytic hypochromic anaemia.     

POTATO TUBER YIELD AND QUALITY AS AFFECTED BY RATES AND SOURCES OF POTASSIUM FERTILIZER

Among the major nutrients, potassium (K) not only improves yields but also improves quality parameters. Field experiments were conducted to assess the comparative effect of sources and rates of K fertilizer on potato yield and quality on a sandy loam soil. Graded doses of potassium, i.e., 0, 150 and 225 kg ha^?1 K₂O from sulfate and muriate of potash were applied in triplicate. Recommended dose of nitrogen (N) and phosphorus (P) applied uniformly. Significant increase in tuber yield was observed with 150 kg ha^?1 K₂O from both the sources over control. Increase in tuber yield with 225 kg ha^?1 K₂O was statistically non significant compared to 150 kg ha^?1. The dry matter and specific gravity were more affected with sulfate of potash (SOP) than muriate of potash (MOP). The quality parameters like dry matter, specific gravity, starch contents, vitamin C, chips color and taste were improved with K application.     

Retrospective Study on Seroprevalence of Borrelia burgdorferi Antibodies in Horses in Minnesota

To determine the seroprevalence of Borrelia burgdorferi antibodies in horses in Minnesota, the database of the Veterinary Diagnostic Laboratory, University of Minnesota, was searched over a 10-year period (May 2001 to May 2010). A total of 1,260 equine serum samples submitted by 112 veterinary clinics were tested using an indirect fluorescent antibody test. Samples with titers of ≥1:320 were considered positive. The average rate of seroprevalence was 58.7%, indicating high exposure of horses to B burgdorferi in Minnesota. Our results indicate that borreliosis should be considered as a differential in cases of horses with undiagnosed musculoskeletal or neurologic disease.     

Isolation of nematicidal compounds from Tagetes patula L. yellow flowers: structure-activity relationship studies against cyst nematode Heterodera zeae infective stage larvae

Bioassay-guided isolation studies on the extracts of yellow flowers of Tagetes patula L. against the Heterodera zeae were carried out to identify phytochemicals lethal to this economically important cyst nematode. In vitro investigation of a polar extract and fractions showing activity led to the isolation of phenolic compounds (flavonoids and phenolic acids). In the nonpolar extract, a few fatty acids, their methyl esters, and thiophenes (including α-terthienyl) were detected. In studies of compounds obtained commercially, α-terthienyl and gallic and linoleic acids showed 100% mortality at concentrations of 0.125% after 24 h. Assessment of structure-activity relationships revealed that an increase in the number of hydroxyl groups in phenolic acids increased the activity; with fatty acids, activity depended on chain length and the number and position of double bonds. Crude extracts of the flowers of different colors also have promising activity.     

Improved productivity and zinc content for maize grain by different zinc fertilization techniques in calcareous soils

Scientific management of nutrients along with several other crop management practices are required for sustainable production of maize (Zea mays L.). Zinc (Zn) status of maize grown in Potohar plateau, Pakistan was monitored. A two-year field study was conducted at two sites to assess the yield of maize cultivars, i.e., hybrid (cv. NARC-2704) and local (cv. Agaiti-2002) and Zn requirement of leaves and grain using various Zn application methods. Zinc was applied by three ways, i.e., broadcast and band placement each at 3, 6 and 9 kg Zn ha^?1; and foliar at 0.5, 1.0 and 1.5 kg Zn ha^?1 along with recommended basal fertilization. Cultivars' response varied to Zn rates, application techniques and sites. The maximum increase in grain yield (two-year mean) at NARC site was: broadcast – hybrid, 17% and local, 13%; band placement – hybrid, 18% and local, 16%; and foliar – hybrid, 15% and local, 13%. Corresponding response at Pindi Gheb site was: broadcast – hybrid, 20% and local, 16%; band placement – hybrid, 21% and local, 17%; and foliar – hybrid, 17% and local, 15%. Zn concentration in leaves and grain of both cultivars also increased to varying extent as a result of applied Zn rates and techniques. Fertilizer Zn requirement for near-maximum grain yield (kg ha^?1) was: broadcast – hybrid, 4.6 and local, 1.9; band placement – hybrid, 1.8 and local, 1.5; and foliar – hybrid, 0.28 and local, 0.26.