Characterization of vitamin B12 compounds from commercially available fish roe products

Although fish roes (or the egg-laden ovary) are seafood products with high nutritional value and are considered abundant in vitamins including vitamin B₁₂, nevertheless, the detailed properties of vitamin B₁₂ have not been fully elucidated in fish roe products. Vitamin B₁₂ content was determined using C18-reversed-phase high-performance liquid chromatography after purification of samples from immunoaffinity columns. Salmon egg-laden ovary products (sujiko), salmon roe products (ikura), dried mullet roe products (bottarga), and white sturgeon roe products (caviar) were found to contain substantial levels of vitamin B₁₂ (more than approximately 15 µg/100 g wet weight). Interestingly, high levels of vitamin B₁₂ per 100 g wet weight have been measured in pollack roe products (tarako) and flying fish roe products (tobiko). Liquid chromatography–electrospray-ionization/tandem mass spectrometry analysis revealed that vitamin B₁₂ was the predominant corrinoid in the evaluated fish roe products examined, while no inactive corrinoid compounds were identified. These results suggest that commercially available fish roe products could be an important source of vitamin B₁₂ for use as a dietary product for humans.

     

Long Term Trend of Chemical Constituents in Tokyo Metropolitan Area in Japan

In recent years, acid rain has been a social problem all over the world. In Japan, it is also a big problem especially in the metropolitan area. Then, we have measured major ions such as H⁺, Na⁺, NH₄ ⁺, K⁺, Mg²⁺, Ca²⁺, Cl^?, NO₃ ^?, and SO₄ ^2? in precipitation and dry deposition samples which had been collected at 9 sampling sites at Hiyoshi, Mita, Kashiwa, Shiki, Fujisawa, Yokosuka, Mitaka, Hachiouji, and Ashikaga in Tokyo Metropolitan area for 10 years since 1990. The average pH of precipitation in their sites was 4.56 (n=1906). As the results of multiple regression analysis showed that pH of precipitation was determined by 5 ions such as NH₄ ⁺, nssCa²⁺(non sea salt calcium), nssCl^?(non sea salt chloride), NO₃ ^?, nssSO₄ ^2?(non sea salt sulfate) in the most of the sampling sites. Therefore, it is very important to investigate the behavior of these ions to understand the acidification of rain in Tokyo Metropolitan area. In this study, a long term trend of each ion concentration in precipitation and wet deposition was also investigated the base on the data we had observed at 7 sites for 10 years by the statistical method.     

Origin identification of dried seaweed product “nori” by PCR–RFLP analysis of Pyropia yezoensis in the internal transcribed spacer ITS-1 region

Nucleotide sequences in internal transcribed spacer (ITS)-1 region derived from dried nori products produced in Japan, China, and the Republic of Korea were compared. Thalli contained in the Japanese products were genetically homogenous, and their nucleotide sequences in ITS-1 were identical to those of the reference strains of Pyropia yezoensis f. narawaensis. In Chinese products, the thalli were related to P. yezoensis strain Minomiasakusa. In contrast, the thalli in the Korean products were genetically heterogeneous, and several different P. yezoensis strains and other Pyropia spp. were used for dried nori products. In some thalli produced in both China and Korea, the DNA sequences of the ITS-1 region were identical with that of Japan, suggesting that the cultivar strains might have been transplanted from Japan to China in recent years. The 432-bp-long nucleotide sequences in the ITS-1 region of thalli derived from Japanese origin were cleaved to two restriction fragments at 154 and 278 bp by cleavage of PCR-amplified products using MspI. Conversely, almost all of the corresponding sequences derived from China and Korea were lacking MspI or other restriction patterns, except for nori products from some areas that cultivate a closely related strain to the Japanese cultivar.     

Thermal insulation properties of wood-based sandwich panel for use as structural insulated walls and floors

Thermal insulation and warmth-keeping properties of thick plywood-faced sandwich panels with low-density fiberboard (plywood-faced sandwich, PSW), which were developed as wood-based structural insulation materials for walls and floors, are comprehensively clarified. The properties focused on were thermal conductivity (λ), thermal resistance (R), and thermal diffusivity (D). The results for PSW panels were compared with those for commercial wood-based boards, solid wood, and commercial insulators. The λ values were measured for PSW panels and their core and face elements. As a result, the composite theory of λ was found to be appropriate for PSW composites, because the calculated/experimental λ ratios were approximately 90%. The λ values for PSW panels with densities of 340 kg/m³ (PSW350) and 410kg/m³ (PSW400) were 0.070 and 0.077W/mK, respectively. The R values for PSW350 and PSW400 were 1.4 and 1.2m²K/W, and the D values were 0.00050 and 0.00046m²/h, respectively. Consequently, the PSW provided thermal insulation properties superior to those of the boards and in terms of warmth-keeping properties were greatly advantageous over the insulators. These advantages were due to the moderate densities of PSW panels. The PSW panel with sufficient thickness showed remarkably improved thermal resistance compared with those of the boards.     

Selenoneine,total selenium,and total mercury content in the muscle of fishes

Levels of the selenium-containing imidazole compound selenoneine and overall organic selenium were measured in the muscle of fishes by speciation analysis. The method involves monitoring ⁸²Se levels by liquid chromatography inductively coupled plasma mass spectroscopy using a gel filtration column. Selenoneine levels were found to be highest in swordfish muscle (concentration 2.8 nmol/g tissue). The selenoneine contents of bigeye tuna, Pacific bluefin tuna, albacore, yellowfin tuna, and alfonsino muscle were 1.3–2.6 nmol/g tissue. In muscle of these fishes, most organic selenium (9–42%) was present as selenoneine. In other fish species, such as Pacific sardine, greeneye, skipjack, Pacific mackerel, horse mackerel, red sea bream, and Japanese barracuda, selenoneine levels were 0.1–1.4 nmol/g tissue, accounting for 3–34% of organic selenium. In contrast, muscle of Japanese conger, Japanese anchovy, chum salmon, Pacific saury, white croaker, and marbled sole contained levels of selenoneine below the level of detection (<0.05 nmol/g tissue). Mercury and selenium contents were 0.01–5.12 nmol/g tissue and 1.4–19.1 nmol/g tissue. The Se-to-Hg molar ratio varied from species to species, ranging from 1 for swordfish to 217 for marbled sole.     

Elastic moduli and stiffness optimization in four-point bending of wood-based sandwich panel for use as structural insulated walls and floors

Several wood-based sandwich panels with low-density fiberboard core were developed for structural insulated walls and floors, with different face material, panel thickness, and core density. The elastic moduli with and without shear effect (E _L, E ₀) and shear modulus (G_b) were evaluated in four-point bending. Generally, the stiffer face, thicker panel, and higher core density were advantageous in flexural and shear rigidity for structural use, but the weight control was critical for insulation. Therefore, optimum designs of some virtual sandwich structures were analyzed for bending stiffness in relation to weight for fixed core densities, considering the manufactured-panel designs. As a result, the plywood-faced sandwich panel with a panel thickness of 95 mm (PSW-T100), with insulation performance that had been previously confirmed, was most advantageous at a panel density of 430 kg/m³, showing the highest flexural rigidity (E _L I = 13 × 10⁻⁶ GNm²) among these panels, where E _L, E ₀, and G _b were 3.5, 5.5, and 0.038 GN/m², respectively. The panel was found to be closest to the optimum design, which meant that its core and face thickness were optimum for stiffness with minimum density. The panel also provided enough internal bond strength and an excellent dimensional stability. The panel was the most feasible for structural insulation use with the weight-saving structure.     

In-plane shear properties of the wood-based sandwich panel as a small shear wall evaluated by the shear test method using tie-rods

 The fundamental in-plane shear properties were investigated for the wood-based sandwich panel of plywood-overlaid low-density fiberboard (SW) manufactured at a pilot scale to develop it as a shear wall. The shear test method using tie-rods standardized for shear walls was applied to SW with dimensions of 260 mm square and 96 mm thick as a small shear wall and to plywood (PW) and thick low-density fiberboard (FB). The shear modulus and shear strength of PW, FB, and SW were determined. To measure the shear deformation angle, a displacement meter and strain-gauge were used. The shear moduli of PW (0.68 g/cm³) and FB (0.25–0.35 g/cm³) were 460 and 21–58 MPa/rad, respectively. The shear modulus of SW as a composite was analyzed. Some experimental models of SW were proposed (i.e., rigid-α, rigid-β, flexible, and semirigid models). The shear modulus of SW (0.35–0.40 g/cm³) evaluated based on the rigid-α and semirigid models were 73–89 and 109–125 MPa/rad, respectively. The theoretical shear modulus of SW was calculated to be 110–129 MPa/rad. Received: May 9, 2001 / Accepted: June 26, 2002 RID="*" ID="*" Part of this report was presented at the 50th Annual Meeting of the Japan Wood Research Society, Kyoto, Japan, April 2000; and the 5th Pacific Rim Bio-Based Composite Symposium, Canberra, Australia, December 2000 Acknowledgments The authors express our deep gratitude to Mr. Noritoshi Sawada (Hokushin Co.), Dr. Wong Cheng, and their cooperative members for their expert technical support for the preparation of manufacturing the thick fiberboard and sandwich panel. We are grateful also to Drs. Min Zhang, Kenji Umemura, Wong Ee Ding, and Guangping Han for their great help and advice in manufacturing the thick panels. The authors are grateful to Hokushin Co. for the fiber and resin and to Ishinomaki Gouhan Co. for the plywood. We thank Mr. Makoto Nakatani for his expert assistance when preparing the specimens for the shear test. Funding provided by the Research Fellowship of the Japan Society for the Promotion of Science for Young Scientists as a JSPS Research Fellow is also gratefully acknowledged.