Infection of human enterovirus 71 in immunosuppressed rhesus monkeys

AIM: To investigate the infection process of human enterovirus 71 (EV71) in a rhesus monkey model of immunosuppression.METHODS: The monkey immunosuppression model was established by oral administration of cyclosporin A at a daily dose of 15 mg/kg for 7 days. Fourteen days after inoculation of EV71 FY23 strain via respiratory and digestive tracts, the infectious monkeys were sacrificed, and then the pathological changes of systemic organs were observed and the viral analysis in different tissues was performed.RESULTS: Compared with the experimental group without cyclosporin A, more positive results were observed in the immunosuppressed monkeys, such as more severe clinical symptoms, higher viral load/antigen expression and more pathological changes in the organs.CONCLUSION: The findings of the viral multiplication in the host provide evidence for evaluation of EV71 vaccine, and also indicate the delimitation for the population using EV71 vaccine in future.     

夏训对优秀皮划艇运动员铁调素等铁营养状况以及IL-6的影响

目的：探讨夏训对优秀运动员铁调素等铁营养状况以及IL-6的影响。方法：广东省优秀皮划艇运动员26人(男15人，女11人)参加测试，调查夏训前和夏训后血红蛋白、铁调素、可溶性转铁蛋白受体、血清铁蛋白、可溶性转铁蛋白受体/log铁蛋白、血清铁、总铁结合力、转铁蛋白饱和度以及白介素6变化情况。结果：夏训后，男运动员组铁调素和可溶性转铁蛋白受体极显著上升(P<0.01)、血清铁蛋白极显著下降(P<0.01)，可溶性转铁蛋白受体/log铁蛋白显著上升(P<0.05)，总铁结合力显著下降(P<0.05)，白介素6极显著上升(P<0.01)。女运动员组血红蛋白显著降低(P<0.05)，铁调素和可溶性转铁蛋白受体极显著上升(P<0.01)，血清铁蛋白极显著下降(P<0.01)，可溶性转铁蛋白受体/log铁蛋白极显著上升(P<0.01)，白介素6极显著上升(P<0.01)。结论：夏训可引起运动员炎症因子升高、铁调素升高，导致功能铁不足；铁调素介导的铁代谢紊乱可能是运动性低血色素发生的重要原因。     

花生组培苗嫁接技术的研究

为解决花生组培再生苗及转基因苗驯化移栽成活率低的难题,本试验以花生实生苗作为砧木,对花生嫁接技术进行了研究。试验结果表明:超净台内无菌嫁接效果较好,以再生苗或实生苗为接穗进行无菌嫁接,其成活率均达90%以上,明显高于室内嫁接成活率(71%～72%);无菌嫁接时12～15d苗龄的砧木效果最好;将无菌嫁接苗在培养基中培养3～5d后进行驯化移栽为最佳时期;不同花生基因型嫁接苗成活率在87%～95%;嫁接苗移栽田间后,其成活率高达94%,且100%结果。     

影响猪采食量的因素

随着猪生产性能的提高，猪的采食量成为限制生产性能的主要因素之一，猪的采食量直接影响遗传潜力的发挥和生产效益，因此，受到养猪者和研究人员的重视。影响猪采食量的因素很多，包括环境、饲料、健康、遗传特性等，从生理上讲，它主要由下丘脑的摄食中枢和饱中枢控制，下丘脑通过各种调节因子对食欲进行着精细的调节，而且这些调控因子之间存在着交互作用。作者主要对猪群采食量调节机制、影响猪采食量的因素及调控猪采食量的基因研究进展做一综述。     

Effects of grape seed extract on meat color and premature browning of meat patties in high-oxygen packaging

This study investigated the effects of grape seed extract (GSE) on fresh and cooked meat color and premature browning (PMB) in ground meat patties (85% beef and 15% pork back fat) packaged under high-oxygen modified atmospheres (HiOx-MAP).  The GSE was added to patties at concentrations of 0, 0.10, 0.25, 0.50 and 0.75 g kg^–1.  This study evaluated the surface color, pH, lipid oxidation, and total viable counts (TVC) of raw patties, and the internal color and pH of patties cooked to a temperature of 66 or 71°C over 10-day storage at 4°C.  Compared with the control (0 g kg^–1 GSE), GSE improved the color stability (P<0.05) and significantly inhibited the lipid and myoglobin oxidation of raw patties from day 5 to 10, but GSE had no effect (P>0.05) on TVC.  Patties containing 0.50 and 0.75 g kg^–1 GSE cooked to 66°C exhibited greater (P<0.05) interior redness than the control and reduced the PMB of cooked patties in the late storage stage.  These results suggested that 0.50 and 0.75 g kg^–1 GSE can improve fresh meat color and minimize PMB of HiOx-MAP patties.     

桂花种植密度调控对生长量的影响

试验采用‘金桂’桂花品种苗木在山坡地种植,在不同生长阶段采用疏移措施调整桂花密度。结果表明：不同生长阶段密度调控对桂花的生长具有极显著影响,以初植密度（株行距）1 m×1 m,在4年树龄及7年树龄时适时疏移2次的生长量最大;初植密度（株行距）2 m×2 m,在树龄6年疏移1次的生长量次之;初植密度（株行距）3 m×3 m,在生长期内不疏移的生长量最小。     

歙县茶产业现状及对策

通过分析当前歙县茶产业现状以及存在的问题,提出促进该地区茶叶产业提升对策,包括推进茶树良种引进、土地流转、茶园基础设施建设、茶农培训,提升茶园效益,提升茶叶加工水平,实施品牌战略以及促进茶产业与旅游业的结合等。     

养殖场鸡粪在蔬菜上的合理安全施用技术研究

研究以不同方式施用生物有机肥、腐植酸和集约化养殖禽畜粪肥等对菜心产量和品质的影响,探讨适用于集约化菜地的禽畜粪合理施用技术。设无机肥、生物有机肥、腐植酸、鸡粪和复合芽孢杆菌等不同组合处理,进行连续六茬菜心田间试验,研究比较不同处理对菜心产量、品质、大肠杆菌群数量和重金属累积的影响。结果表明,连续六茬3000 kg/hm2鸡粪+复合芽孢杆菌处理菜心产量均为最高且均显著高于单施无机肥处理,复合芽孢杆菌堆沤鸡粪处理产量次之。所有处理间可溶性糖与维生素C含量变化均未表现出明显规律,鸡粪+芽孢杆菌处理除第一茬外的其它五茬菜心亚硝酸盐含量和大肠杆菌群数量均较低。六茬菜心Cu、Zn和As含量均没有明显差别且未超过国家食品卫生标准。在施用无机肥基础上配施适量重金属含量低于我国肥料重金属限值的鸡粪,并在菜心生长过程中淋施复合芽孢杆菌,不但提高菜心产量,而且保障菜心品质安全,是集约化养殖禽畜粪肥在集约化菜地的合理安全施用方式。     

Survey and Analysis of Common Diseases of Laboratory Monkeys in Kunming Area

Survey and analysis of common diseases of laboratory monkeys are great significance to understand disease epidemiology, and formulate a set of scientific and reasonable measures for prevention and control disease, to improve the quantity and quality of laboratory monkeys, to ensure the accuracy of the animal experiment results. Through the investigation of the sick and dead animals of a large-scale laboratory monkeys breeding facility in Kunming area in 2014, the animals were classified depends on the main diseases, and the diseases were classified according to adult and juvenile animals, data were analyzed by Excel Office 2010 software. The results showed the common diseases of laboratory monkeys included the digestive system, respiratory system, trauma, reproductive system, and locomotor system disease, which were 47.45%, 13.06%, 12.10%, 11.46% and 8.92% separately; Common diseases included diarrhoea, soft tissue injuries, abortion, lobar pneumonia, dysentery, arthritis, intestinal pneumatosis, chronic colitis, and extreme bad nutrition, the ratio were 18.47%, 11.15%, 8.91%, 8.28%, 8.28%, 7.96%, 5.10%, 5.10% and 5.09% separately. Comprehensive analysis of common diseases and disease occurrence causes of the laboratory monkeys, suggesting that providing better quality laboratory monkeys for scientific research only by constantly improving the scientific breeding management level, constantly strengthening veterinary capacity, mastering the theoretical knowledge and scientific diagnosis method.