蛋雏鸡传染性喉气管炎的诊治

鸡传染性喉气管炎(ILT)是由鸡传染性喉气管炎病毒引起的以剧烈咳嗽、气喘、高度呼吸困难和气管内有血样渗出物等为特征的一种急性呼吸道传染病.育成蛋鸡感染该病多有报道,而蛋雏鸡感染发病比较少见.本文以一起48日龄海兰褐蛋雏鸡传染性喉气管炎病例为题材,从发病情况、临床症状、病理剖检变化、诊断、防治等几个方面对该病做了详尽阐述.     

猪败血性链球菌与附红细胞体混合感染病例

猪败血性链球菌病是猪链球菌感染引起的一种急性败血性传染病,猪附红细胞体病是由猪附红细胞体寄生于猪红细胞而引起的一种散在的热性、溶血性传染病.本文以某猪场这2种病混合感染病例为题材,从发病基本情况、临床症状、剖检变化、实验室检验、防治措施等6个方面对该混合感染病例做了详尽的阐述.     

APPEARANCE OF THE SACROILIAC JOINT IN VENTRODORSAL RADIOGRAPHS OF THE NORMAL CANINE PELVIS

Radiology of the canine sacroiliac (SI) joint was investigated by obtaining ventrodorsal radiographs of cadaveric pelves and bone specimens (n = 40) marked with solder wire or radiopaque paint to demonstrate the orientation of the sacroiliac joint in various radiographic views. In the following retrospective radiographic study (n = 94 survey sacroiliac joint radiographs), central projections of the sacrum were noted to ease identification of the dorsal, middle and ventral joint components, whereas angled projections of the sacrum had advantages when assessing the cranial joint components. Radiographic assessment was already successful in juvenile dogs older than 5 months. Failure in tracing the outlines of the joint space of the sacroiliac joints is not consistent with alteration of the SI joint. Rather, it was felt that alterations would have assisted in identifying the contours of the SI joints because of an increase in mineralization.     

Respiration and parturition affected by conditional overexpression of the Ca2+-activated K+ channel subunit, SK3

In excitable cells, small-conductance Ca2+-activated potassium channels (SK channels) are responsible for the slow after-hyperpolarization that often follows an action potential. Three SK channel subunits have been molecularly characterized. The SK3 gene was targeted by homologous recombination for the insertion of a gene switch that permitted experimental regulation of SK3 expression while retaining normal SK3 promoter function. An absence of SK3 did not present overt phenotypic consequences. However, SK3 overexpression induced abnormal respiratory responses to hypoxia and compromised parturition. Both conditions were corrected by silencing the gene. The results implicate SK3 channels as potential therapeutic targets for disorders such as sleep apnea or sudden infant death syndrome and for regulating uterine contractions during labor.     

BKCa-Cav channel complexes mediate rapid and localized Ca2+-activated K+ signaling

Large-conductance calcium- and voltage-activated potassium channels (BKCa) are dually activated by membrane depolarization and elevation of cytosolic calcium ions (Ca2+). Under normal cellular conditions, BKCa channel activation requires Ca2+ concentrations that typically occur in close proximity to Ca2+ sources. We show that BKCa channels affinity-purified from rat brain are assembled into macromolecular complexes with the voltage-gated calcium channels Cav1.2 (L-type), Cav2.1 (P/Q-type), and Cav2.2 (N-type). Heterologously expressed BKCa-Cav complexes reconstitute a functional "Ca2+ nanodomain" where Ca2+ influx through the Cav channel activates BKCa in the physiological voltage range with submillisecond kinetics. Complex formation with distinct Cav channels enables BKCa-mediated membrane hyperpolarization that controls neuronal firing pattern and release of hormones and transmitters in the central nervous system.     

Gonadotropin‐Releasing Hormone Administration to Dairy Cows without a Corpus Luteum 4 Weeks after Calving Increases Reproductive Performance

This field study investigated whether the administration of a single dose of gonadotropin‐releasing hormone (GnRH) to dairy cows without a corpus luteum (CL) 4 weeks after calving can improve reproductive performance. Holstein dairy cows underwent ultrasonography to assess the presence of ovarian structures at 29.2 ± 5.2 days post‐partum, and cows were divided into two main groups based on the presence (CL group, n = 230) or absence (non‐CL group, n = 460) of a CL. The non‐CL group was further randomly divided into two subgroups based on the administration of GnRH (non‐CL GnRH group, n = 230) or no GnRH (non‐CL control group, n = 230). Subsets of cows from non‐CL control (n = 166) and non‐CL GnRH (n = 175) groups received a second ultrasonography at 44.5 ± 5.4 days post‐partum to assess CL formation. The percentage of cows with CL at the second ultrasonography was greater in the non‐CL GnRH group (70.9%) than in the non‐CL control group (53.0%, p = 0.0006). The hazard of the first post‐partum insemination by 150 days in milk (DIM) was higher in the CL group than in the non‐CL control group (hazard ratio [HR]: 1.36, p = 0.001). The probability of a pregnancy to the first insemination was higher in non‐CL GnRH (odds ratio [OR]: 1.50, p = 0.04) and CL groups (OR: 1.55, p = 0.03) compared to the non‐CL control group. Furthermore, the hazard of pregnancy by 210 DIM was higher in non‐CL GnRH (HR: 1.30, p = 0.01) and CL (HR: 1.51, p = 0.0001) groups than in the non‐CL control group. In conclusion, administration of GnRH to dairy cows without a CL 4 weeks after calving was associated with an increase in ovulation and improved reproductive performance.