Seroprevalence and risk factors associated with Chlamydophila spp. infection in ewes in the northeast of Algeria

A cross-sectional study was carried out to estimate prevalence of Chlamydophila spp. antibodies and to investigate risk factors associated with chlamydial infection in 552 ewes between March 2011 and January 2012 in the province of Constantine. Anti-Chlamydophila antibodies were detected using an indirect enzyme-linked immunosorbent assay kit in 24.5 % of examined sera. Of the herds, 70.4 % had at least one seropositive animal. A pretested structured questionnaire was administered in order to collect information on individual animal health and herd management practices. Chi-square analysis and multivariable logistic regression model were used to identify risk factors related to Chlamydophila seropositivity. Univariable analysis revealed 17 variables with p?<?0.25 that were offered to the multivariable logistic regression model which in turn identified 12–23 months age group (OR?=?5.903, 95 % CI (OR)?=?1.690; 20.618) and not using disinfectants (OR?=?2.099, 95 % CI (OR)?=?1.314; 8.065) as risk factors for Chlamydophila spp. seropositivity. Moreover, occurrence of stillbirth problem (OR?=?3.682, 95 % CI (OR)?=?1.825; 7.430) and 5–10 % mortality rate in young lambs (OR?=?2.584, 95 % CI (OR)?=?1.058; 6.310) were significantly associated with seropositivity to Chlamydophila spp. On the other hand, availability of veterinary service was identified as a protective factor (OR?=?0.161, 95 % CI (OR)?=?0.051; 0.511).     

Nutrient and heavy metals in decaying harvest residue needles on drained blanket peat forests

Harvest residue decomposition can significantly contribute to nutrient and heavy metal exports to receiving water courses. This study monitors the nutrient and heavy metal dynamics in decaying Sitka spruce and lodgepole pine harvest residue needles on Atlantic blanket peat forests in the west of Ireland. Using the litterbag method, harvest residue was placed both within and between furrows in two uncut forest and two clear-cut sites. On the clear-cut sites, the litterbags were positioned outside the harvest residue piles (i.e. brash windrows). Over the 2-year monitoring period, the needles decomposed slower at the clear-cut sites than the uncut forest sites, with mass losses of 46–55 and 58–77 %, respectively. Approximately 20 % less phosphorous (P) was released from the decaying needles at the clear-cut sites, while nitrogen (N) was released only at the uncut sites. Tree species was a significant factor contributing to nutrient and heavy metal release and accumulation patterns, with higher concentrations of aluminium (Al), nickel (Ni), cadmium (Cd) and zinc (Zn) in the decaying spruce needles than in pine. Conversely, the spruce needles showed accelerated depletion of calcium (Ca) and magnesium (Mg) relative to the pine. The harvest residue needle positioning (inside furrow/between furrows) and the site soil characteristics contributed significantly to Al transformations in spruce needles and iron (Fe) in both spruce and pine needles, with more accumulation occurring inside the furrows where Al and Fe contents of the peat were high. Manganese (Mn) was released from the needles in three of the four sites with a total release of over 90 % within 2 years. In the remaining site, where the Mn content of the peat was high, an accumulation of Mn in the needles was observed. The decomposition of needles on blanket peat catchments may be a significant source of P to receiving water courses, owing to their fast release of P, but not a likely source for N export.     

利用乳成分分析仪与近红外光谱仪比较分析牛奶中的乳脂肪和乳蛋白

采用乳成分分析仪和近红外光谱仪比较分析了牛奶中的乳脂肪和乳蛋白。分析结果表明,二者没有显著差异,近红外光谱仪可以代替乳成分分析仪用来检测牛奶中的乳脂肪和乳蛋白。     

Genetic variability and estimates of heritability in sorghum (Sorghum bicolor L.) genotypes grown in a semiarid zone of Sudan

A field experiment was conducted to evaluate nine genotypes of sorghum grown for two consecutive cropping seasons in 2006 and 2007 under rain-fed conditions in a semiarid zone at the Research Farm of El Fasher Research Station, Sudan. The objective of the study was to assess genetic variability and heritability among sorghum genotypes using phenotypic morphological traits. A randomized complete block design with four replications was used for the experiment. The grain yield (kg ha^?1), the number of heads/plant, followed by straw yield (kg ha^?1) had the highest genotypic coefficients of variation in both seasons. High heritability (above 95%) was shown for plant height in both seasons. High genetic advance was reported in straw yield, 1000-grain weight and days to flowering in both seasons. Highly significant differences among genotypes were found for all characters. The high-yielding genotype was Adv-Edo-CWS (E-9) with grain yields of 2780.6 kg ha^?1. Grain yield was significantly and positively correlated with straw yield (r = 0.91), number of heads/plant (r = 0.69), plant height (r = 0.53) and 1000-grain weight (r = 0.36). However, it was significantly and negatively correlated with days to 50% flowering (r = ?0.21). Based on their positive association with grain yield, the character’s straw weight, number of heads/plant, plant height and 1000-grain weight would be the preferable selection criteria for sorghum improvement program in the country.     

Role of GABAB Receptor and L-Arg in GABA-Induced Vasorelaxation in Non-diabetic and Streptozotocin-Induced Diabetic Rat Vessels

Background:

Hypertension is considered an independent risk factor for cardiovascular mortality in diabetic patients. The present study was designed to determine the role of gamma amino butyric acid B (GABA_B) receptor and L-arginine (L-Arg) in GABA-induced vasorelaxation in normal and streptozotocin-induced diabetic rat vessels.

Methods:

Diabetes was induced by a single i.p. injection of streptozotocin (STZ, 60 mg/kg). Eight weeks later, superior mesenteric arteries of all groups were isolated and perfused according to the McGregor method.

Results:

Baseline perfusion pressure of STZ diabetic rats was significantly higher than non-diabetic rats in both intact and denuded endothelium. In the presence of faclofen, a selective GABA_B receptor blocker, GABA-induced relaxation in intact and denuded endothelium mesenteric beds of STZ diabetic rats was suppressed, but this response in non-diabetic rats was not suppressed. Our results showed that in the presence of L-Arg, a nitric oxide precursor, GABA induced vasorelaxation in both diabetic and non-diabetic vessels.

Conclusion:

From the results of this study, it may be concluded that the vasorelaxatory effect of GABA in diabetic vessel is mediated by the GABA_B receptor and nitric oxide, but it seems that in non-diabetic vessel GABA_B receptor does not play any role in GABA-induced vasorelaxation, but nitric oxide induced GABA relaxation in non-diabetic vessel. Key Words: Gamma amino butyric acid (GABA), Diabetes, GABA_B receptor     

Anti-Oxidant Activities,Chemical Attributes and Fruit Yield of Peach Cultivars as Influenced by Foliar Application of Ascorbic Acid

Peach fruit is enriched with natural antioxidants but oxidation caused by biotic and abiotic stresses, reduce these antioxidants and consequently effect the quality and yield of the fruit. Therefore, an experiment was conducted to investigate the role of ascorbic acid to sustain the natural antioxidant activity, improve the fruit quality and yield of peach cultivars (Early Grand and Florida King). The peach cultivars, Early Grand and Florida King were tested against four levels of ascorbic acid (200, 400, 600 and 800?ppm) and compared with control as water spray. The foliar application of ascorbic acid significantly influenced anti-oxidant activity and physico-chemical attributes of peach cultivars. However, the foliar application of ascorbic acid @ 800?ppm significantly increased the fruit weight, fruit yield, fruit firmness, total soluble solids, titratable acidity, antioxidant activity of fruit peel, with least percent infected fruits and fruit juice pH as compared to the rest of the treatments. Similarly, significant variation was recorded for peach cultivars regarding anti-oxidant activity and various physico-chemical attributes. The maximum fruit weight, fruit yield, fruit firmness, total soluble solids, titratable acidity and TSS/TA ratio were noted in fruit of cultivar Florida King. On other side the foliar treated fruit of peach cultivar Early Grand had the highest percent infected fruits, fruit juice pH and antioxidant activity of fruit peel. It is concluded from the significant findings of present research that the peach cultivar Florida King performed better in terms of maximum fruit yield, whereas the cultivar Early Grand produced better quality fruits with high anti-oxidant activity when treated with 800?ppm of ascorbic acid as foliar spray.     

Effect of animal age,postmortem chilling rate,and aging time on meat quality attributes of water buffalo and humped cattle bulls

The study was aimed to investigate the influence of animal age, post‐slaughter chilling rate, and aging time on meat quality of M. longissimus dorsi (LD) of water buffalo (Bubalus bubalis) and humped cattle (Bos taurus indicus) bulls. After slaughtering, one side of carcasses was subjected to rapid chilling (RC) (0 ± 2°C) and other side was hanged in controlled room temperature (25 ± 2°C) for 3 hr, then allowed to the chiller (0 ± 2°C). The meat quality traits were analyzed at 1, 7, and 14 days of storage. It was noted that rapidly chilled carcasses from the younger animals of both species missed the ideal pH/temperature window, which affects the toughness of the meat. Buffalo meat presented higher shear force, color L* values, and lower b* value as compared to the cattle meat. Moreover, meat shear force values decreased while all color coordinates and cooking loss values increased with lengthening the storage time in both age groups of cattle and buffalo. In conclusion, the tenderness of cattle meat was superior to that of buffalo and RC adversely affect the shear force values of young cattle and both age groups of buffalo bulls.     

Folic acid and flaxseed oil supplements in Ossimi ewes: effect on body weight changes,progesterone profile,blood chemistry,and litter traits

Tropical Animal Health and Production - The aim was to explore the impact of periconceptional folic acid or flaxseed oil (FXO) supplementation on fertility, progesterone profile, and blood...