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排序方式: 共有118条查询结果,搜索用时 15 毫秒
1.
Immunogenic properties of Landrace pigs selected for resistance to mycoplasma pneumonia of swine 下载免费PDF全文
Liushiqi Borjigin Tomoyuki Shimazu Yuki Katayama Meihua Li Takumi Satoh Kouichi Watanabe Haruki Kitazawa Sang‐gun Roh Hisashi Aso Kazuo Katoh Takafumi Uchida Yoshihito Suda Akiko Sakuma Mituru Nakajo Keiichi Suzuki 《Animal Science Journal》2016,87(3):321-329
Mycoplasma pneumonia of swine (MPS) lung lesions and immunogenic properties were compared between a Landrace line that was genetically selected for reduced incidence of pulmonary MPS lesions, and a non‐selected Landrace line. The MPS‐selected Landrace line showed significantly lower degrees of pulmonary MPS lesions compared with the non‐selected Landrace line. When changes in immunity before and after vaccination were compared, the percentage of B cells in the peripheral blood of the MPS‐selected Landrace line was significantly lower than that of the non‐selected line. Furthermore, the concentration of growth hormone and the mitogen activity of peripheral blood mononuclear cells in the MPS‐selected Landrace line showed significantly (P < 0.05) lower increases after vaccination than the non‐selected line. Conversely, the concentration of peripheral blood interferon (IFN)‐γ and salivary immunoglobulin A (IgA) after Mycoplasma hyopneumoniae vaccination was significantly higher in the MPS‐selected Landrace line than in the non‐selected line. Gene expression of toll‐like receptor (TLR)2 and TLR4 was significantly higher in the MPS‐selected Landrace line in immune tissues, with the exception of the hilar lymph nodes. The present results suggest that peripheral blood IFN‐γ, salivary IgA TLR2, and TLR4 are important immunological factors influencing the development of MPS lesions. 相似文献
2.
Immunogenic properties and mycoplasmal pneumonia of swine (MPS) lung lesions in Large White pigs selected for higher peripheral blood immune capacity 下载免费PDF全文
Liushiqi Borjigin Tomoyuki Shimazu Yuki Katayama Meihua Li Takumi Satoh Kouichi Watanabe Haruki Kitazawa Sang‐gun Roh Hisashi Aso Kazuo Katoh Takafumi Uchida Yoshihito Suda Akiko Sakuma Mituru Nakajo Keiichi Suzuki 《Animal Science Journal》2016,87(5):638-645
Immunogenic properties and mycoplasmal pneumonia of swine (MPS) lung lesions were compared between the immunity‐selected Large White line and the non‐selected Large White line. The selected Large White line showed a higher level of pulmonary MPS lesions compared with the non‐selected Large White line. Subsequent to vaccination, the percentage of natural killer cells and T cells (CD3+CD4+CD8‐ and CD3+CD4?CD8+ T cells) were significantly increased in the non‐selected line but remained unchanged in the immunity‐selected Large White line. Secretion of Mycoplasma hyopneumoniae vaccine‐specific immunoblogulin G and phagocyte activity in peripheral blood were significantly higher in the immunity‐selected Large White line than in the non‐selected line. Expression of interleukin (IL)‐4 and IL‐6 messenger RNA in hilar lymph nodes was significantly lower in the immunity‐selected Large White line than in the non‐selected line. However, expression of IL‐10 in all immune tissues was significantly higher in the immunity‐selected Large White line. These results suggest that the selection for high immunity was not effective in increasing resistance to MPS lung lesions. 相似文献
3.
Mycoplasma pneumonia of swine (MPS) resistance and immune characteristics of pig lines generated by crossing an MPS pulmonary lesion selected Landrace line and a highly immune capacity selected Large White line 下载免费PDF全文
Liushiqi Borjigin Tomoyuki Shimazu Yuki Katayama Kouichi Watanabe Haruki Kitazawa Sang‐gun Roh Hisashi Aso Kazuo Katoh Takafumi Uchida Yoshihito Suda Akiko Sakuma Mituru Nakajo Keiichi Suzuki 《Animal Science Journal》2016,87(8):972-981
To understand the influence of crossbreeding on Mycoplasma pneumonia of swine (MPS) resistance and immune characteristics, two crossbred lines were characterized. One crossbred line, LaWa, was generated by crossing the MPS pulmonary lesion selected Landrace line (La) and the highly immune‐selected Large White line (Wa). The second crossbred line, LaWb, was generated by crossing the La line and the nonselected Large White line (Wb). The crossbred LbWb line (nonselected Landrace line × nonselected Large White line) and the La line were used as controls. The LaWa and LaWb lines had an intermediate level of MPS lung lesions between La and LbWb lines, although the difference was not statistically significant. After stimulation with sheep red blood cells (SRBCs), the LaWb and LaWa lines showed immune characteristics similar to that of the La line; the number of monocytes in peripheral blood increased, while B cells, T cells, secretion of SRBC‐specific immunoglobulin G, and interleukin (IL)‐13 decreased. Additionally, the number of natural killer (NK) cells and the expression of IL‐4 and IL‐17 were significantly higher in the LaWb and LaWa lines, respectively. These data suggested that crossbreeding of La and Wa lines resulted in the inheritance of some of the selected immune responses. 相似文献
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AIM: To investigate the effects and mechanisms of sphingosine-1-phosphate receptor-2 (S1P2R)on lipopolysaccharide (LPS)-induced acute lung injury (ALI). METHODS: ALI model was induced by intratracheal administration of LPS in both wild-type mice and S1P2R -deficient mice. The pathological changes in the lung tissues were observed, and the protein concentration, total cell number, neutrophil ratio, TNF-α level and IL-6 level were determined in the bronchoalveolar lavage fluid (BALF) 24 h after LPS injection. In order to investigate the mechanisms of S1P2R in LPS-induced ALI, 10 min before LPS injection, both wild-type mice and S1P2R -deficient mice were injected with nitric oxide synthase inhibitor by tail vein injection, the pathological changes of the lung tissues were observed, and the protein concentration and total cell number in BALF were determined 12 h after LPS injection. RESULTS: Compared with wild-type mice, S1P2R -deficient mice showed more severe LPS-induced ALI, and the protein concentration, neutrophils and inflammatory cytokines in BALF were significantly increased in S1P2R -deficient mice. Administration of nitric oxide synthase inhibitor Nω-L-nitro-arginine methyl ester protected S1P2R -deficient mice from aggravation of ALI. CONCLUSION: S1P2R mediates the protection from LPS-induced ALI possibly through inhibiting nitric oxide synthase. 相似文献
6.
Yunting?FangEmail author Per?Gundersen Rolf?D.?Vogt Keisuke?Koba Fusheng?Chen Xi?Yun?Chen Muneoki?Yoh 《Journal of Forest Research》2011,16(5):341-350
Data have been compiled from published sources on nitrogen (N) fluxes in precipitation, throughfall, and leaching from 69
forest ecosystems at 50 sites throughout China, to examine at a national level: (1) N input in precipitation and throughfall,
(2) how precipitation N changes after the interaction with canopy, and (3) whether N leaching increases with increasing N
deposition and, if so, to what extent. The deposition of dissolved inorganic N (DIN) in precipitation ranged from 2.6 to 48.2 kg N ha−1 year−1, with an average of 16.6 kg N ha−1 year−1. Ammonium was the dominant form of N at most sites, accounting for, on average, 63% of total inorganic N deposition. Nitrate
accounted for the remaining 37%. On average, DIN fluxes increased through forest canopies, by 40% and 34% in broad-leaved
and coniferous forests, respectively. No significant difference in throughfall DIN inputs was found between the two forest
types. Overall, 22% of the throughfall DIN input was leached from forest ecosystems in China, which is lower than the 50–59%
observed for European forests. Simple calculations indicate that Chinese forests have great potential to absorb carbon dioxide
from the atmosphere, because of the large forest area and high N deposition. 相似文献
7.
Muneoki Yoh 《Water, air, and soil pollution》2001,130(1-4):661-666
The soil nitrogen cycle was investigated for several watershed forests of which stream waters have distinctively different nitrate (NO3 ?) concentrations. In a watershed with stream water NO3 ? of more than 100 µM, soil NO3 ? content increased even beneath the rooting zone, revealing "nitrogen saturation" status. A laboratory soil experiment demonstrated that the proportion of net NO3 ? production to CO2 production was largely regulated by a soil C/N ratio, suggesting a key parameter for NO3 ? abundance. In the respective watershed soils, little nitrogen was actually present as NO3 ? above a soil C/N ratio of 20. The annual mean soil temperature recorded at the sites was correlated with a shift in the C/N ratio in watershed soils (a soil C/N ratio increase of 0.5 per 1°C decrease) along the stream NO3 ? gradient of 30 fold. The results suggest that soil microbial metabolisms affected by C/N ratio may be a direct agent regulating NO3 ? leaching from watersheds under the influence of an atmospheric nitrogen load and climate. 相似文献
8.
Noguchi Y Nagata H Koganei H Kodera Y Hiroto M Nishimura H Inada Y Matsushima A 《Journal of agricultural and food chemistry》2002,50(12):3540-3543
An aminopeptidase, Jc-peptidase, was purified from Japanese cedar pollen by seven steps, including precipitation with ammonium sulfate, ion-exchange chromatography, gel filtration, hydrophobic interaction chromatography on phenyl-agarose, and high-performance liquid chromatography. Purified Jc-peptidease has a molecular weight of 42 kDa and hydrolyzes the synthetic substrates of L-phenylalanyl-4-methylcoumaryl-7-amide (Phe-MCA) with Km = 5 x 10(-5) M, Tyr-MCA with Km = 7 x 10(-4) M, Leu-MCA with Km = 1 x 10(-3) M, and Met-MCA with Km = 1 x 10(-3) M. Other MCA analogues such as Arg-MCA or Glu-MCA failed to serve as its substrates. The activity was inhibited in the presence of phebestin, [(2S,3R)-3-amino-2-hydroxy-4-phenylbutanoyl-L-valyl]-L-phenylalanine, with Ki = 4.7 x 10(-5) M, or bestatin, [(2S,3R)-3-amino-2-hydroxy-4-phenylbutanoyl]-L-leucine, with Ki = 1.1 x 10(-4) M. According to amino acid sequence analysis, the N-terminal amino group seems to be blocked. The physiological function of the aminopeptidase (Jc-peptidase) has not been clarified in vivo. 相似文献
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