广玉兰叶片抗氧化活性评价

用乙酸乙酯从广玉兰(Magnolia grandiflora)叶片中萃取出的活性物质,在进行主要成分理化检识鉴定后,以人工抗氧化剂PG(没食子酸丙酯)和天然抗氧化剂PA(植酸)为对照,测定了其体外抗氧化活性,并用两种体系DPPH·(二苯代苦味酰基自由基)和卵磷脂进行评价.结果表明,提取物含有黄酮类化合物和酚类化合物.提取物稀释10倍(92.00 mg·mL-1)时,对过氧化氢的清除率达最大,为29.41%,在稀释20倍(46.00 mg·mL-1)时,抑制活性氧活性达最大,达500活力单位,稀释50倍(18.40 mg·mL-1)时,非酶体系的总抗氧化能力达最大,为270活力单位.DPPH·体系和卵磷脂体系评价结果表明,在提取物稀释50倍时(18.40 mg·mL-1),对DPPH·的清除率为92.69%,比PG强,对脂质过氧化的抑制作用达最大,达56.67%,与PA相当,因此,抗氧化效果最好.     

Chemical composition and free radicals restraining activity of extracts from three Manglietia species leaves

The extracts from leaves of Manglietia insignis （Wall） Blume, Manglietia chingii Dandy and Manglietia yuyuanensis Law were prepared by organic solvent extraction and their components were analyzed by GC/MS and quantified. Meanwhile, the free radicals restraining activities were detected. The 21 compounds in M. insignis, 36 compounds in M. chigii and 20 compounds in M. yuyuanensis were identified. There were 11 common components in the extracts from three Manglietia species, and 12 components in two Manglietia species. The results of relative contents of every component in three extracts showed that the main constituents of M. insignis were terpenoids and alkene, amounting to 38.93%, followed by alkane （28.18%）, the nitrogen containing compounds （15.73%） and aromatic compounds （7.23 %）. The main constituents of leaf extract from M. chingii were the terpenoids and alkene, carboxylic acid, alkane and aromatic compounds, amounting to 30.22%, 14.17%, 13.87% and 13.29%, respectively, The main constituents of M. yuyuanensis were alcohol compounds, the terpenoids and alkene, and aromatic compounds, amounting to 28.00%, 25.38% and 18.00% respectively. The results showed that the three extracts had strong function of restraining oxygen free radicals. The ultra oxygen anions activity was restrained at the highest level, when the three extracts were diluted by hundred-fold, whereas the restraining capacity of hydroxyl free radicals reached maximum, when the three extracts were diluted by twenty-fold. The above results provide scientific evidences for further approaching the ecological healthy function of three MangUetia species     

4种含笑叶片提取物对大豆油的抗过氧化活性

木兰科(Magnoliaceae)的含笑属(Michelia)树种是一类芳香植物,具有巨大的应用潜力.近年来,对含笑叶片成分及其活性的研究取得了重要进展(明军等, 2004).在其提取物的抗氧化活性研究方面,国内外都有大量报道(Deepa et al., 2006;Singh et al., 2006;Kosar et al., 2003;Bartolome et al., 2004).Puertas等(2005)从2种木兰科植物中提取出抗氧化物质,测定其有很强的抗氧化活性.     

三种木莲叶片提取物成分及其抑制氧自由基活性研究

用有机溶剂萃取法从三种木莲叶片中分离获得的提取物,经GC/MS进行成分分析和定量测定.再进行抗氧自由基活性测定.从红花木莲(Manglietia insignis (Wall) Blume)、桂南木莲(Manglietia chingii Dandy)和乳源木莲(Manglietia yuyuanensis Law)中分别鉴定出21、36和20种化合物,其中11种成分为三种木莲所共有,12种成分为两种木莲所共有.各成分的相对含量为红花木莲:萜烯类化物38.93%,烷烃类28.18%,含氮化合物15.73%,芳香族化合物7.23%.桂南木莲:萜烯类30.22%,酸类14.17%,烷烃类13.87%,芳香族化合物13.29%;乳源木莲:醇类28.00%,萜烯类25.38%,芳香族化合物18.00%. 研究结果还表明,三种木莲的提取物均有较强的抑制氧自由基的功能.在稀释100倍时,抑制超氧阴离子活力最强,稀释20倍时,抑制羟自由基活力达最大.本研究结果为进一步探索三种木莲的保健功能提供科学依据.图1表5参15.     

彩叶植物金叶绣线菊组织培养研究

金叶绣线菊具有叶色金黄夺目和花期长的特点,近几年广泛用于园林绿化中．为达到快速繁殖的目的,我们对金叶绣线菊的幼嫩茎段进行离体培养研究,金叶绣线菊快繁的最佳途径方法是;以MS为基本培养基,以IAA、6-BA及NAA的不同含量组合进行对比试验,结果表明：诱导分化阶段以培养基MS 6-BA 0．3mg／L LAA0．2mg／L诱导效果最好,生根阶段以MS 6．BA0．3mg／L NAA 0．5mg／L效果最好,初步实现了金叶绣线菊的快速繁殖,为金叶绣线菊广泛地应有于生产奠定了基础。