三种木莲叶片提取物成分及其抑制氧自由基活性研究

用有机溶剂萃取法从三种木莲叶片中分离获得的提取物,经GC/MS进行成分分析和定量测定.再进行抗氧自由基活性测定.从红花木莲(Manglietia insignis (Wall) Blume)、桂南木莲(Manglietia chingii Dandy)和乳源木莲(Manglietia yuyuanensis Law)中分别鉴定出21、36和20种化合物,其中11种成分为三种木莲所共有,12种成分为两种木莲所共有.各成分的相对含量为红花木莲:萜烯类化物38.93%,烷烃类28.18%,含氮化合物15.73%,芳香族化合物7.23%.桂南木莲:萜烯类30.22%,酸类14.17%,烷烃类13.87%,芳香族化合物13.29%;乳源木莲:醇类28.00%,萜烯类25.38%,芳香族化合物18.00%. 研究结果还表明,三种木莲的提取物均有较强的抑制氧自由基的功能.在稀释100倍时,抑制超氧阴离子活力最强,稀释20倍时,抑制羟自由基活力达最大.本研究结果为进一步探索三种木莲的保健功能提供科学依据.图1表5参15.

Chemical composition and free radicals restraining activity of extracts from three Manglietia species leaves

The extracts from leaves of Manglietia insignis (Wall) Blume,Manglietia chingii Dandy and Manglietia yuyuanensis Law were prepared by organic solvent extraction and their components were analyzed by GC/MS and quantified. Meanwhile,the free radicals re-straining activities were detected. The 21 compounds in M. insignis,36 compounds in M. chigii and 20 compounds in M. yuyuanensis were identified. There were 11 common components in the extracts from three Manglietia species,and 12 components in two Manglietia species. The results of relative contents of every component in three extracts showed that the main constituents of M. insignis were terpenoids and alkene,amounting to 38.93%,followed by alkane (28.18%),the nitrogen containing compounds (15.73%) and aromatic compounds (7.23 %). The main constituents of leaf extract from M. chingii were the terpenoids and alkene,carboxylic acid,alkane and aromatic compounds,amounting to 30.22%,14.17%,13.87% and 13.29%,respectively. The main constituents of M. yuyuanensis were alcohol compounds,the terpenoids and alkene,and aromatic compounds,amounting to 28.00%,25.38% and 18.00% respectively. The results showed that the three extracts had strong function of restraining oxygen free radicals. The ultra oxygen anions activity was restrained at the highest level,when the three extracts were diluted by hundred-fold,whereas the restraining capacity of hydroxyl free radicals reached maximum,when the three extracts were diluted by twenty-fold. The above results provide scientific evidences for further approaching the ecological healthy function of three Manglietia species