动物防御素研究进展

防御素(defensin)是一族具有广谱抗微生物和细胞毒活性的多肽,是天然免疫的重要介质,属内源性抗生素肽(endogenous antibiotic peptides)家族.介绍了动物防御素的分布、结构、表达、作用机理等方面的内容.     

兔防御素基因在毕赤酵母中的串连表达及抑菌机理研究

根据毕赤酵母Pichia pastoris密码子偏爱性重编兔防御素基因(Neutrophils peptide-1,pNP-1),设计4条引物搭桥合成.将pNP-1克隆到pPICZα-A载体,使用同尾酶Xho Ⅰ和SalⅠ在重组载体上构建多价串联体[pNP-1(2×)]和[ pNP-1(3×)],分别对这3个基因进行毕...     

Assembling a physical map of the genome by marker sequences

lntroductionAfteragenomehasbeenfragmented,thefrag-mentsclonedtogenerateagen0miclibrary.ltisnecessaryt0assemblethecIonedfragmentsinthesameIinearorderasfoundinthechromos0mesfromwhichtheyarederived.OnemethodofIinkingcIonedfragmentswaschromosomewalking(Benderetal.1983),whichwasoriginaIlydevelopedfOrtheis0Iationofgenesequenceswhosefunctionwasunknownbutwhosegeneticlocationwasknown.Forthepurposesofmapping,asinglecIonedfragmentwasseIectedandusedasapr0betodetectotherclonesintheIibrary,withwhichthep…     

pE3—mMT—I—cDNA重组质粒的分离纯化及对根癌农杆菌LBA4404的转化

本文通过对ｐＥ３－ｍＭＴ－Ｉ－ｃＤＮＡ克隆质粒的分离纯化及鉴定，确定了ｍＭＴ－Ｉ基因确实已整合进入ＰＥ３植物双元表达载体上。并通过三亲株杂交法将ｐＥ３－ｍＭＴ－Ｉ基因转化根癌农杆菌ＬＢＡ４４０４。原位杂交结果表明，转化后的根癌农杆菌菌株ＬＢＡ４４０４中带有ｍＭＴ－Ｉ基因。     

13种哺乳动物OTR密码子使用偏性及其聚类分析

目的研究13种哺乳动物催产素受体基因（OTR）密码子使用偏性,并探讨其影响因素;通过对偏性情况进行聚类分析,研究其与系统发育的关系．方法计算RSCU,CBI,ENC等密码子偏性指标,与可能的几种影响因素做相关性分析,采用SPASS15．0软件,以相对同义密码子使用度为变量对偏性情况进行聚类分析．结果与结论1）CUG,GUG,GCC,UUC,AUC,CGC为哺乳动物OTR使用频率较高的密码子;2）密码子第3位碱基的GC含量是影响哺乳动物OTR密码子偏性的主要因素,基因的GC含量次之,而芳香族氨基酸的含量和蛋白疏水水平对其密码子使用偏性的影响不大;3）对于功能相同的基因,亲缘关系相近的物种密码子使用偏性指标较接近,但某些物种仍有一定差异．     

TESTING THE MUTAGENICITY OF FIVE PESTICIDES WITH MICRONUCLEUS TECHNIQUE OF TRADESCANTIA PALUDOSA

lNTRoDUCTIoNTradescantiapaludOsa,asanindicatorplantgrowinginthesubtropiczone,ab-sorbsmutation-inducedsubstancesfromwater,soilandatmosphere.Thisinducesthechromosomeofthepollenfemalece1ltobreakanddamageandtoformthemicronucleusinthetetradbeingasanmonitoringindexoftheenvironmentalpol-lution[l3.Itis,asabiologicalmonitoringmethod,differentfromthephysical,chem-icalanalysisones-TheauthorsappliedtheTradescantiapaludosamicronucleustech'niquetotestthemutagenicityofthepesticides:Decis,Sumicid,Cyperm…     

A study on the genetic noxiousness of trinitrotoluene

lntroductionTrinitrotoIuene(TNT),animportantmaterialtosyn-thesizegunpower,isoneofthecommonandancientindustrypoisonsandhasaharmfuInesstomanyorgansandsystemsuchastheliver,bloodsystem,andcrystaIIinelenscontactedwithit(A1thouse1983).Butthereportsstudyingonthechronicgeneticnox-ionsnessofTNTarereIativeIyraresofar.Therefore,itisverynecessarytostudydeeplyonthemutageniceffectsofTNTandtocalcuIatethegeneticharrnfuI-nessofTNTtovariousanimals(Draggan1978).lnthispaper,wehavestudiedthatthemutagencity…     

Molecular cloning and analysis of the partial sequence of Rhinopithecus roxellanae growth hormone gene

IntroductionGrowth hormone gene (GH) of the vertebrate, a 22kDa pfot6in hormone synthesized in anterior pituitarygland, was r6quired for preadult groWth (Franchimontand Burger 1975i Barinaga et al. 1985). The genesenCOding rat, human, bovine, porcine, ovine, turkeyand duck GH have been well studied at both proteinand gene levels (Page et al. 1981, DeNotO et al. 1981,WOychik et aL 1982; Wze et aL 1987i Byrne et al.1987; FOSter et aL 1990). GroWth hormone gene isalso a member Of a mul…