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采用辣根过氧化物酶(HRP)与3,3′,5,5′—四甲基联苯胺(TMB)—H2O2作为信号示踪系统,建立了一种可视化抗体微阵列检测菲律宾蛤仔过敏原的方法。对孵育时间、抗体浓度等免疫条件进行了优化。采用改良双抗体夹心法,将菲律宾蛤仔过敏原的兔多克隆抗体固定于琼脂糖三维芯片上,依次加入待检样品、菲律宾蛤仔鼠多克隆抗体和HRP-羊抗鼠IgG,孵育后加TMB显色,肉眼观察后,用平板扫描仪获取扫描图象,采用GenePixPro6.0软件分析灰度值。试验结果表明,该方法最低可检出10ng/mL的菲律宾蛤仔过敏原,片内平均变异系数(CV)为5.99%,片间为10.3%;香肠及蟹棒中3个不同浓度的加标回收率为73.54%~95.44%;4℃存放4个月内抗体微阵列玻片活性保持稳定。该方法不需要大型精密仪器,结果直观可见,可以发展为对多种过敏原进行同时检测,具有良好的实用和推广价值。  相似文献
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Color vision, accommodation and visual acuity in the largemouth bass   总被引:1,自引:1,他引:1  
The largemouth bass is a sight feeder and one of the most popular freshwater fish for lure fishing. While lures a have variety of colors and forms, there is no information on the color vision and form perception in the largemouth bass. We recorded S-potentials from the isolate retinae of the largemouth bass to examine color vision electrophysiologically. The occurrence of the C-response provided direct evidence for the possession of color vision. L-responses recorded indicated a high spectral sensitivity to red. The direction and distance of lens movement of a specimen of 43 cm in fork length indicated a horizontal visual axis and the near point at 13.5 cm, respectively. The visual acuity calculated from the cone density and the focal distance of the lens was 0.10. Histological characteristics of the retina indicated that the eyes are well adapted to both form and movement perceptions.  相似文献
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以可视化的方式,通过对海洋现象和过程、海洋环境状况的虚拟表达以及海洋开发利用的真实卫星影像三维模拟,成为认识海洋、管理海洋、开发海洋、利用海洋、保护海洋的有效手段。本文以福建省海洋管理三维可视化系统研发为例,研究了海洋管理所涉及到的4种类型数据及其特征,以及在海洋管理中对三维可视化技术应用的需求。介绍了SkyLine三维可视化建模和实现工具的原理、架构与实现。探讨了海洋管理三维可视化系统开发关键技术及实现过程。系统根据海洋管理实际的需求包含三维地图操作、基础信息、海洋资源、海岸线、海岛、海域使用、海洋环境、海洋灾害、社会经济、统计分析模块,实现了对海洋资源、海洋开发利用和防灾减灾三维可视化的管理。  相似文献
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江涛  谌志新  刘平 《南方水产》2007,3(4):60-64
将网箱模型在水槽实验中获得的不同波高、流速状态下的实验数据,还原成网箱在水中空间6自由度运动,通过虚拟现实建模语言(VRML)和Java的联合使用,开发了可视化系统,使得网络用户不仅能访问该系统,且可选择不同实验数据进行交互访问。系统运行时,可实时切换场景的视角,进行多方位观察和研究。  相似文献
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Induction of stress protein (HSP70) was studied in goldfish (Carassius auratus) reared with bluegills (Lepomis macrochirus), a predator of small fish. HSP70 was identified by Western blotting and quantified by optical density after SDS-PAGE. The expression of HSP70 was significantly enhanced in the brains of goldfish reared with bluegills for 6 and 12 h in a single tank. The hepatopancreas and the kidney were not affected by the treatment. When goldfish were separated from bluegills with a partition net (1×1 cm mesh size), this protein also increased in the brains after 6 h but then returned to the control level after 12 h. When the goldfish and bluegills were kept in separate tanks and were not able to see each other but were connected by circulating water, HSP70 levels in the goldfish were unaffected. Immunohistochemical observations indicated that an anti-HSP70 antibody was found to react predominantly with the optic and vagal lobes of the brains. These results suggest that visual perception plays a primary role in enhancement of HSP70 expression in the goldfish reared with bluegills.  相似文献
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SUMMARY: In bluefin tuna culture, a high mortality of juveniles is caused by bumping into the tank and net-pen walls at dawn. This bumping can possibly be attributed to visually disoriented behavior of the fish. To examine this possibility, the authors carried out retinomotor response experiments with juvenile bluefin tuna (50.7–96.8 mm total length, at which they were transferred from a indoor tank to a sea net-pen) and measured ambient light intensity at the culture site at dawn. The light intensity at which the transition from scotopic to photopic vision takes place was 7.52 lx and the time taken by the transition was 15 min. At dawn, the ambient light intensity rapidly increased from scotopic light intensity level and attained photopic light intensity level in 10 min. This incompatibility of the retinal adaptation with the change in the ambient light intensity could cause the visual disorientation of the fish. It is therefore possible that the visually disoriented juveniles cannot control their high power swimming and thus bump into the walls at dawn.  相似文献
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