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为了研究栀子、龙胆、茵陈水煎剂对獭兔胆汁分泌和离体胆囊平滑肌肌条运动的影响,通过对獭兔实施胆管插管术,建立胆汁引流模型,应用 BL-420E 生物机能实验系统观察记录上述中药对胆汁分泌的影响;并取獭兔胆囊平滑肌,应用 HW-400S 恒温平滑肌槽和 BL-420E 生物机能实验系统,观察记录上述中药对胆囊平滑肌运动的影响。结果显示,栀子水煎剂可显著增加胆汁的分泌(P <0.05)和胆囊平滑肌收缩张力(P <0.05);龙胆和茵陈水煎剂可显著增加胆汁的分泌(P <0.05),但对胆囊平滑肌收缩张力作用不显著(P >0.05)。说明栀子、龙胆、茵陈水煎剂均可促进獭兔胆汁分泌,且栀子可提高胆囊平滑肌收缩张力,但龙胆、茵陈水煎剂对胆囊平滑肌收缩张力影响不显著。 相似文献
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【目的】探究准噶尔双峰驼哺乳期幼驼和成年母驼粪便菌群结构组成及多样性变化,为幼驼肠道健康发育提供重要依据。【方法】选取6~7岁体况相近的健康成年母驼12峰,以及出生日期相近(3月龄)的哺乳期母幼驼12峰,母驼与幼驼在相同饲养环境下饲养。采用直肠取粪法采集粪样,用于粪便内容物细菌16S rDNA的V3-V4区测序,并对测序数据进行相应分析。【结果】母驼粪便菌群Chao1和Shannon指数均显著高于幼驼(P<0.05);在门水平上,母驼和幼驼粪便中位于前十的菌均为厚壁菌门、拟杆菌门、疣微菌门、变形菌门、软壁菌门、螺旋体门、TM7、放线菌门、蓝藻菌门和纤维杆菌门,其中幼驼粪便中变形菌门和软壁菌门丰度均极显著高于母驼(P<0.01);在科水平上,母驼与幼驼粪便中位于前十的菌均为瘤胃球菌科、消化链球菌科、毛螺菌科、梭菌科、克里斯滕森菌科、理研菌科、疣微菌科、拟杆菌科、艰难杆菌科和普雷沃氏菌科,其中母驼粪便中消化链球菌科和梭菌科的丰度均极显著高于幼驼(P<0.01),而幼驼粪便中毛螺菌科的丰度极显著高于母驼(P<0.01);在属水平上,母驼与幼驼粪便中位于前十的菌属均为梭菌属、颤螺旋菌属、瘤胃球菌属、梭菌科-梭菌属、阿克曼菌属、5-7 N15、CF231、苏黎世杆菌属、梭菌科-SMB53和密螺旋体菌属,其中母驼粪便中的梭菌属、梭菌科-梭菌属和苏黎世杆菌属的丰度均极显著高于幼驼(P<0.01)。【结论】成年母驼粪便菌群多样性显著高于哺乳期幼驼,且稳定性较好;母驼和幼驼的粪便在门、科、属水平下的主导菌属种类均一致,但是丰度均存在差异。 相似文献
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Intravenous sufentanil‐midazolam versus sevoflurane anaesthesia in medetomidine pre‐medicated Himalayan rabbits undergoing ovariohysterectomy 下载免费PDF全文
Patricia Hedenqvist Marianne Jensen‐Waern Åsa Fahlman Ragnvi Hagman Anna Edner 《Veterinary anaesthesia and analgesia》2015,42(4):377-385
ObjectiveTo compare physiological effects of sufentanil-midazolam with sevoflurane for surgical anaesthesia in medetomidine premedicated rabbits.Study designProspective, randomized controlled experimental study.AnimalsEighteen female Himalayan rabbits, weight 2.1 ± 0.1 kg.MethodsPremedication with 0.1 mg kg−1 medetomidine and 5 mg kg−1 carprofen subcutaneously, was followed by intravenous anaesthetic induction with sufentanil (2.3 μg mL−1) and midazolam (0.45 mg mL−1). After endotracheal intubation, anaesthesia was maintained with sufentanil-midazolam (n = 9) or sevoflurane (n = 9). Ovariohysterectomy was performed. Intermittent positive pressure ventilation was performed as required. Physiological variables were studied perioperatively. Group means of physiologic data were generated for different anaesthetic periods. Data were compared for changes from sedation, and between groups by anova. Post-operatively, 0.05 mg kg−1 buprenorphine was administered once and 5 mg kg−1 carprofen once daily for 2–3 days. Rabbits were examined and weighed daily until one week after surgery.ResultsSmooth induction of anaesthesia was achieved within 5 minutes. Sufentanil and midazolam doses were 0.5 μg kg−1 and 0.1 mg kg−1, during induction and 3.9 μg kg−1 hour−1 and 0.8 mg kg−1 hour−1 during surgery, respectively. End-tidal sevoflurane concentration was 2.1% during surgery. Assisted ventilation was required in nine rabbits receiving sufentanil-midazolam and four receiving sevoflurane. There were no differences between groups in physiologic data other than arterial carbon dioxide. In rabbits receiving sevoflurane, mean arterial pressure decreased pre-surgical intervention, heart rate increased 25% during and after surgery and body weight decreased 4% post-operatively. Post-operative problems sometimes resulted from catheterization of the ear artery.ConclusionSevoflurane and sufentanil-midazolam provided surgical anaesthesia of similar quality. Arterial blood pressure was sustained during sufentanil-midazolam anaesthesia and rabbits receiving sevoflurane lost body weight following ovariohysterectomy. Mechanical ventilation was required with both anaesthetic regimens.Clinical relevanceAnaesthesia with sufentanil-midazolam in medetomidine premedicated healthy rabbits is useful in the clinical and the research setting, as an alternative to sevoflurane. 相似文献
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Anatomic Study of Female Sterility of Pinus tabulaeformis Carr. 总被引:3,自引:0,他引:3
ChengPengjun LiFenglan ZhengCaixia 《中国林学(英文版)》2003,5(1):13-19
The anatomic research on the mutant clone of Pinus tabulaeformis Carr.in the seed orchard in Xingcheng,Liaoning Provinces was carried out.The female cone of the mutant clone looked like normal,but its ovules degenerated in early stage.This paper trics to find out the reason and time of ovule abortion.It seems that the ovule abortion is probably caused by female sterility because the microspores of this mutant clone were normal.Through the serial observations on the one-year-old macrosporangiates and the ovules of two-year-old female cones of mutant and normal clone,it is found that the reason of ovule abortion in mutant clone is the failure of the mitosis of free nuclei in the female gametophyte,and the time is about in the early April. 相似文献
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Bao Ren-yan Zheng Cai-xia 《中国林学(英文版)》2005,7(4):16-19
To determine the relationship between female sterility of Pinus tabulaeformis Carr. and plant hormones, content changes of several endogenous plant hormones in a female-sterile clone and a normal clone were analyzed during the key period of female gametophyte abortion in mutant clones. The change of ABA content showed a similar pattern between normal and mutant clones, but the ABA content of the mutant clone was always much higher than that of the normal clone. In the normal clone, the IAA content decreased significantly at the early stage of the period and reached its minimum during the first ten days of April and stabilized thereafter. The endogenous ZR levels increased sharply at the early stages and reached a maximum in the middle of April, then decreased dramatically. The IAA and ZR contents of the mutant clone did not change perceptively through the entire period. We conclude that the high levels of ABA and the deficit of IAA and ZR may be the reasons of female gametophyte abortion in the female-sterile clones of P. tabulaeformis. 相似文献
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[目的]揭示平欧杂种榛主栽品种的需冷量和需热量,更好地指导生产做好区域化栽培。[方法]本研究以16个平欧杂种榛主栽品种的1年生枝条为试材,以野生平榛1年生枝条为对照,通过水培观察的方法,首次对平欧杂种榛的雌、雄花序和叶芽进行需冷量和需热量研究。[结果]表明:平欧杂种榛不同器官的需冷量由低到高依次为雄花序336 1 176 h、雌花序504 1 344 h、叶芽672 1 512 h;需热量由低到高依次为雄花序687.0 998.5GDH℃、雌花序998.5 3 496.1 GDH℃、叶芽2 546.5 4 180.3 GDH℃;不同品种、器官在刚满足最低蓄冷要求时需热量最高,随着蓄冷时间的延长,需热量逐渐减少。[结论]首次报道了平欧杂种榛主栽品种雄、雌花序和叶芽的需冷量和需热量数据,其中,达维、平欧48号等品种花器官需冷量较低,辽榛2号、平欧545号等品种花器官需冷量较高;玉坠、平欧69号等品种花器官需热量较低,辽榛1号、辽榛4号等品种花器官需热量较高,上述研究丰富了榛属植物开花和休眠解除方面的数据,可为平欧杂种榛的引种和栽培提供参考。 相似文献
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